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-rw-r--r--gnu/packages/bioinformatics.scm94
1 files changed, 87 insertions, 7 deletions
diff --git a/gnu/packages/bioinformatics.scm b/gnu/packages/bioinformatics.scm
index ab9dc185a3..91bb94c549 100644
--- a/gnu/packages/bioinformatics.scm
+++ b/gnu/packages/bioinformatics.scm
@@ -11735,19 +11735,39 @@ using nucleotide or amino-acid sequence data.")
(define-public kallisto
(package
(name "kallisto")
- (version "0.43.1")
+ (version "0.44.0")
(source (origin
- (method url-fetch)
- (uri (string-append "https://github.com/pachterlab/"
- "kallisto/archive/v" version ".tar.gz"))
- (file-name (string-append name "-" version ".tar.gz"))
+ (method git-fetch)
+ (uri (git-reference
+ (url "https://github.com/pachterlab/kallisto.git")
+ (commit (string-append "v" version))))
+ (file-name (git-file-name name version))
(sha256
(base32
- "03j3iqhvq7ya3c91gidly3k3jvgm97vjq4scihrlxh315j696r11"))))
+ "0nj382jiywqnpgvyhichajpkkh5r0bapn43f4dx40zdaq5v4m40m"))))
(build-system cmake-build-system)
- (arguments `(#:tests? #f)) ; no "check" target
+ (arguments
+ `(#:tests? #f ; no "check" target
+ #:phases
+ (modify-phases %standard-phases
+ (add-after 'unpack 'do-not-use-bundled-htslib
+ (lambda _
+ (substitute* "CMakeLists.txt"
+ (("^ExternalProject_Add" m)
+ (string-append "if (NEVER)\n" m))
+ (("^\\)")
+ (string-append ")\nendif(NEVER)"))
+ (("include_directories\\(\\$\\{htslib_PREFIX.*" m)
+ (string-append "# " m)))
+ (substitute* "src/CMakeLists.txt"
+ (("target_link_libraries\\(kallisto kallisto_core pthread \
+\\$\\{CMAKE_CURRENT_SOURCE_DIR\\}/../ext/htslib/libhts.a\\)")
+ "target_link_libraries(kallisto kallisto_core pthread hts)")
+ (("include_directories\\(\\.\\./ext/htslib\\)") ""))
+ #t)))))
(inputs
`(("hdf5" ,hdf5)
+ ("htslib" ,htslib)
("zlib" ,zlib)))
(home-page "http://pachterlab.github.io/kallisto/")
(synopsis "Near-optimal RNA-Seq quantification")
@@ -14057,3 +14077,63 @@ absolute GSEA.")
(description "Ngless is a domain-specific language for
@dfn{next-generation sequencing} (NGS) data processing.")
(license license:expat)))
+
+(define-public filtlong
+ ;; The recommended way to install is to clone the git repository
+ ;; https://github.com/rrwick/Filtlong#installation
+ ;; and the lastest release is more than nine months old
+ (let ((commit "d1bb46dfe8bc7efe6257b5ce222c04bfe8aedaab")
+ (revision "1"))
+ (package
+ (name "filtlong")
+ (version (git-version "0.2.0" revision commit))
+ (source
+ (origin
+ (method git-fetch)
+ (uri (git-reference
+ (url "https://github.com/rrwick/Filtlong.git")
+ (commit commit)))
+ (file-name (git-file-name name version))
+ (sha256
+ (base32 "1xr92r820x8qlkcr3b57iw223yq8vjgyi42jr79w2xgw47qzr575"))))
+ (build-system gnu-build-system)
+ (arguments
+ `(#:tests? #f ; no check target
+ #:phases
+ (modify-phases %standard-phases
+ (delete 'configure)
+ (replace 'install
+ (lambda* (#:key outputs #:allow-other-keys)
+ (let* ((out (assoc-ref outputs "out"))
+ (bin (string-append out "/bin"))
+ (scripts (string-append out "/share/filtlong/scripts")))
+ (install-file "bin/filtlong" bin)
+ (install-file "scripts/histogram.py" scripts)
+ (install-file "scripts/read_info_histograms.sh" scripts))
+ #t))
+ (add-after 'install 'wrap-program
+ (lambda* (#:key inputs outputs #:allow-other-keys)
+ (let* ((out (assoc-ref outputs "out"))
+ (path (getenv "PYTHONPATH")))
+ (wrap-program (string-append out
+ "/share/filtlong/scripts/histogram.py")
+ `("PYTHONPATH" ":" prefix (,path))))
+ #t))
+ (add-before 'check 'patch-tests
+ (lambda _
+ (substitute* "scripts/read_info_histograms.sh"
+ (("awk") (which "gawk")))
+ #t)))))
+ (inputs
+ `(("gawk" ,gawk) ;for read_info_histograms.sh
+ ("python" ,python-2) ;required for histogram.py
+ ("zlib" ,zlib)))
+ (home-page "https://github.com/rrwick/Filtlong/")
+ (synopsis "Tool for quality filtering of Nanopore and PacBio data")
+ (description
+ "The Filtlong package is a tool for filtering long reads by quality.
+It can take a set of long reads and produce a smaller, better subset. It uses
+both read length (longer is better) and read identity (higher is better) when
+choosing which reads pass the filter.")
+ (license (list license:gpl3 ;filtlong
+ license:asl2.0))))) ;histogram.py