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-rw-r--r--gnu/packages/bioinformatics.scm73
1 files changed, 70 insertions, 3 deletions
diff --git a/gnu/packages/bioinformatics.scm b/gnu/packages/bioinformatics.scm
index 546b240f84..73765dafe7 100644
--- a/gnu/packages/bioinformatics.scm
+++ b/gnu/packages/bioinformatics.scm
@@ -10880,8 +10880,8 @@ memory usage and processing time is minimized.")
(license license:expat)))
(define-public r-dropbead
- (let ((commit "cf0be5ae5302684bd03e78ab65b142900bbbb840")
- (revision "1"))
+ (let ((commit "d746c6f3b32110428ea56d6a0001ce52a251c247")
+ (revision "2"))
(package
(name "r-dropbead")
(version (string-append "0-" revision "." (string-take commit 7)))
@@ -10894,7 +10894,7 @@ memory usage and processing time is minimized.")
(file-name (git-file-name name version))
(sha256
(base32
- "1b2lphsc236s1rdzlijxg8yl1jnqpwcvj4x938r89rqpj93jb780"))))
+ "0sbzma49aiiyw8b0jpr7fnhzys9nsqmp4hy4hdz1gzyg1lhnca26"))))
(build-system r-build-system)
(propagated-inputs
`(("r-ggplot2" ,r-ggplot2)
@@ -12646,3 +12646,70 @@ contains
analyze RNA expression genome-wide in thousands of individual cells at
once. This package provides tools to perform Drop-seq analyses.")
(license license:expat)))
+
+(define-public pigx-rnaseq
+ (package
+ (name "pigx-rnaseq")
+ (version "0.0.2")
+ (source (origin
+ (method url-fetch)
+ (uri (string-append "https://github.com/BIMSBbioinfo/pigx_rnaseq/"
+ "releases/download/v" version
+ "/pigx_rnaseq-" version ".tar.gz"))
+ (sha256
+ (base32
+ "168hx2ig3rarphx3l21ay9yyg8ipaakzixnrhpbdi0sknhyvrrk8"))))
+ (build-system gnu-build-system)
+ (arguments
+ `(#:parallel-tests? #f ; not supported
+ #:phases
+ (modify-phases %standard-phases
+ (add-after 'install 'wrap-executable
+ ;; Make sure the executable finds all R modules.
+ (lambda* (#:key inputs outputs #:allow-other-keys)
+ (let ((out (assoc-ref outputs "out")))
+ (wrap-program (string-append out "/bin/pigx-rnaseq")
+ `("R_LIBS_SITE" ":" = (,(getenv "R_LIBS_SITE")))
+ `("PYTHONPATH" ":" = (,(getenv "PYTHONPATH")))))
+ #t)))))
+ (inputs
+ `(("snakemake" ,snakemake)
+ ("fastqc" ,fastqc)
+ ("multiqc" ,multiqc)
+ ("star" ,star)
+ ("trim-galore" ,trim-galore)
+ ("htseq" ,htseq)
+ ("samtools" ,samtools)
+ ("bedtools" ,bedtools)
+ ("r-minimal" ,r-minimal)
+ ("r-rmarkdown" ,r-rmarkdown)
+ ("r-ggplot2" ,r-ggplot2)
+ ("r-ggrepel" ,r-ggrepel)
+ ("r-gprofiler" ,r-gprofiler)
+ ("r-deseq2" ,r-deseq2)
+ ("r-dt" ,r-dt)
+ ("r-knitr" ,r-knitr)
+ ("r-pheatmap" ,r-pheatmap)
+ ("r-corrplot" ,r-corrplot)
+ ("r-reshape2" ,r-reshape2)
+ ("r-plotly" ,r-plotly)
+ ("r-scales" ,r-scales)
+ ("r-summarizedexperiment" ,r-summarizedexperiment)
+ ("r-crosstalk" ,r-crosstalk)
+ ("r-tximport" ,r-tximport)
+ ("r-rtracklayer" ,r-rtracklayer)
+ ("r-rjson" ,r-rjson)
+ ("salmon" ,salmon)
+ ("ghc-pandoc" ,ghc-pandoc)
+ ("ghc-pandoc-citeproc" ,ghc-pandoc-citeproc)
+ ("python-wrapper" ,python-wrapper)
+ ("python-pyyaml" ,python-pyyaml)))
+ (home-page "http://bioinformatics.mdc-berlin.de/pigx/")
+ (synopsis "Analysis pipeline for RNA sequencing experiments")
+ (description "PiGX RNAseq is an analysis pipeline for preprocessing and
+reporting for RNA sequencing experiments. It is easy to use and produces high
+quality reports. The inputs are reads files from the sequencing experiment,
+and a configuration file which describes the experiment. In addition to
+quality control of the experiment, the pipeline produces a differential
+expression report comparing samples in an easily configurable manner.")
+ (license license:gpl3+)))