;;; GNU Guix --- Functional package management for GNU ;;; Copyright © 2014, 2015, 2016 Ricardo Wurmus ;;; Copyright © 2015, 2016 Ben Woodcroft ;;; Copyright © 2015, 2016 Pjotr Prins ;;; Copyright © 2015 Andreas Enge ;;; Copyright © 2016 Roel Janssen ;;; Copyright © 2016 Efraim Flashner ;;; Copyright © 2016 Marius Bakke ;;; ;;; This file is part of GNU Guix. ;;; ;;; GNU Guix is free software; you can redistribute it and/or modify it ;;; under the terms of the GNU General Public License as published by ;;; the Free Software Foundation; either version 3 of the License, or (at ;;; your option) any later version. ;;; ;;; GNU Guix is distributed in the hope that it will be useful, but ;;; WITHOUT ANY WARRANTY; without even the implied warranty of ;;; MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the ;;; GNU General Public License for more details. ;;; ;;; You should have received a copy of the GNU General Public License ;;; along with GNU Guix. If not, see . (define-module (gnu packages bioinformatics) #:use-module ((guix licenses) #:prefix license:) #:use-module (guix packages) #:use-module (guix utils) #:use-module (guix download) #:use-module (guix git-download) #:use-module (guix hg-download) #:use-module (guix build-system ant) #:use-module (guix build-system gnu) #:use-module (guix build-system cmake) #:use-module (guix build-system perl) #:use-module (guix build-system python) #:use-module (guix build-system r) #:use-module (guix build-system ruby) #:use-module (guix build-system trivial) #:use-module (gnu packages) #:use-module (gnu packages autotools) #:use-module (gnu packages algebra) #:use-module (gnu packages base) #:use-module (gnu packages bash) #:use-module (gnu packages bison) #:use-module (gnu packages boost) #:use-module (gnu packages compression) #:use-module (gnu packages cpio) #:use-module (gnu packages curl) #:use-module (gnu packages documentation) #:use-module (gnu packages datastructures) #:use-module (gnu packages file) #:use-module (gnu packages gawk) #:use-module (gnu packages gcc) #:use-module (gnu packages gd) #:use-module (gnu packages gtk) #:use-module (gnu packages glib) #:use-module (gnu packages groff) #:use-module (gnu packages guile) #:use-module (gnu packages haskell) #:use-module (gnu packages image) #:use-module (gnu packages imagemagick) #:use-module (gnu packages java) #:use-module (gnu packages linux) #:use-module (gnu packages logging) #:use-module (gnu packages machine-learning) #:use-module (gnu packages man) #:use-module (gnu packages maths) #:use-module (gnu packages mpi) #:use-module (gnu packages ncurses) #:use-module (gnu packages pcre) #:use-module (gnu packages parallel) #:use-module (gnu packages pdf) #:use-module (gnu packages perl) #:use-module (gnu packages pkg-config) #:use-module (gnu packages popt) #:use-module (gnu packages protobuf) #:use-module (gnu packages python) #:use-module (gnu packages readline) #:use-module (gnu packages ruby) #:use-module (gnu packages serialization) #:use-module (gnu packages statistics) #:use-module (gnu packages tbb) #:use-module (gnu packages tex) #:use-module (gnu packages texinfo) #:use-module (gnu packages textutils) #:use-module (gnu packages time) #:use-module (gnu packages tls) #:use-module (gnu packages vim) #:use-module (gnu packages web) #:use-module (gnu packages xml) #:use-module (gnu packages xorg) #:use-module (gnu packages zip) #:use-module (srfi srfi-1)) (define-public aragorn (package (name "aragorn") (version "1.2.37") (source (origin (method url-fetch) (uri (string-append "http://mbio-serv2.mbioekol.lu.se/ARAGORN/Downloads/aragorn" version ".tgz")) (sha256 (base32 "1c7zxk0h8wsjbix82fmmfyywcq6zn3q9h5y67kcl5y3qal2bv2xr")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; there are no tests #:phases (modify-phases %standard-phases (delete 'configure) (replace 'build (lambda _ (zero? (system* "gcc" "-O3" "-ffast-math" "-finline-functions" "-o" "aragorn" (string-append "aragorn" ,version ".c"))))) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin")) (man (string-append out "/share/man/man1"))) (mkdir-p bin) (copy-file "aragorn" (string-append bin "/aragorn")) (mkdir-p man) (copy-file "aragorn.1" (string-append man "/aragorn.1"))) #t))))) (home-page "http://mbio-serv2.mbioekol.lu.se/ARAGORN") (synopsis "Detect tRNA, mtRNA and tmRNA genes in nucleotide sequences") (description "Aragorn identifies transfer RNA, mitochondrial RNA and transfer-messenger RNA from nucleotide sequences, based on homology to known tRNA consensus sequences and RNA structure. It also outputs the secondary structure of the predicted RNA.") (license license:gpl2))) (define-public bamm (package (name "bamm") (version "1.7.3") (source (origin (method url-fetch) ;; BamM is not available on pypi. (uri (string-append "https://github.com/Ecogenomics/BamM/archive/" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "1f35yxp4pc8aadsvbpg6r4kg2jh4fkjci0iby4iyljm6980sac0s")) (modules '((guix build utils))) (snippet `(begin ;; Delete bundled htslib. (delete-file-recursively "c/htslib-1.3.1") #t)))) (build-system python-build-system) (arguments `(#:python ,python-2 ; BamM is Python 2 only. ;; Do not use bundled libhts. Do use the bundled libcfu because it has ;; been modified from its original form. #:configure-flags (let ((htslib (assoc-ref %build-inputs "htslib"))) (list "--with-libhts-lib" (string-append htslib "/lib") "--with-libhts-inc" (string-append htslib "/include/htslib"))) #:phases (modify-phases %standard-phases (add-after 'unpack 'autogen (lambda _ (with-directory-excursion "c" (let ((sh (which "sh"))) ;; Use autogen so that 'configure' works. (substitute* "autogen.sh" (("/bin/sh") sh)) (setenv "CONFIG_SHELL" sh) (substitute* "configure" (("/bin/sh") sh)) (zero? (system* "./autogen.sh")))))) (delete 'build) ;; Run tests after installation so compilation only happens once. (delete 'check) (add-after 'install 'wrap-executable (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (path (getenv "PATH"))) (wrap-program (string-append out "/bin/bamm") `("PATH" ":" prefix (,path)))) #t)) (add-after 'wrap-executable 'post-install-check (lambda* (#:key inputs outputs #:allow-other-keys) (setenv "PATH" (string-append (assoc-ref outputs "out") "/bin:" (getenv "PATH"))) (setenv "PYTHONPATH" (string-append (assoc-ref outputs "out") "/lib/python" (string-take (string-take-right (assoc-ref inputs "python") 5) 3) "/site-packages:" (getenv "PYTHONPATH"))) ;; There are 2 errors printed, but they are safe to ignore: ;; 1) [E::hts_open_format] fail to open file ... ;; 2) samtools view: failed to open ... (zero? (system* "nosetests"))))))) (native-inputs `(("autoconf" ,autoconf) ("automake" ,automake) ("libtool" ,libtool) ("zlib" ,zlib) ("python-nose" ,python2-nose) ("python-pysam" ,python2-pysam) ("python-setuptools" ,python2-setuptools))) (inputs `(("htslib" ,htslib) ("samtools" ,samtools) ("bwa" ,bwa) ("grep" ,grep) ("sed" ,sed) ("coreutils" ,coreutils))) (propagated-inputs `(("python-numpy" ,python2-numpy))) (home-page "http://ecogenomics.github.io/BamM/") (synopsis "Metagenomics-focused BAM file manipulator") (description "BamM is a C library, wrapped in python, to efficiently generate and parse BAM files, specifically for the analysis of metagenomic data. For instance, it implements several methods to assess contig-wise read coverage.") (license license:lgpl3+))) (define-public bamtools (package (name "bamtools") (version "2.3.0") (source (origin (method url-fetch) (uri (string-append "https://github.com/pezmaster31/bamtools/archive/v" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "1brry29bw2xr2l9pqn240rkqwayg85b8qq78zk2zs6nlspk4d018")))) (build-system cmake-build-system) (arguments `(#:tests? #f ;no "check" target #:phases (modify-phases %standard-phases (add-before 'configure 'set-ldflags (lambda* (#:key outputs #:allow-other-keys) (setenv "LDFLAGS" (string-append "-Wl,-rpath=" (assoc-ref outputs "out") "/lib/bamtools"))))))) (inputs `(("zlib" ,zlib))) (home-page "https://github.com/pezmaster31/bamtools") (synopsis "C++ API and command-line toolkit for working with BAM data") (description "BamTools provides both a C++ API and a command-line toolkit for handling BAM files.") (license license:expat))) (define-public bcftools (package (name "bcftools") (version "1.3.1") (source (origin (method url-fetch) (uri (string-append "https://github.com/samtools/bcftools/releases/download/" version "/bcftools-" version ".tar.bz2")) (sha256 (base32 "095ry68vmz9q5s1scjsa698dhgyvgw5aicz24c19iwfbai07mhqj")) (modules '((guix build utils))) (snippet ;; Delete bundled htslib. '(delete-file-recursively "htslib-1.3.1")))) (build-system gnu-build-system) (arguments `(#:test-target "test" #:make-flags (list "USE_GPL=1" (string-append "prefix=" (assoc-ref %outputs "out")) (string-append "HTSDIR=" (assoc-ref %build-inputs "htslib") "/include") (string-append "HTSLIB=" (assoc-ref %build-inputs "htslib") "/lib/libhts.a") (string-append "BGZIP=" (assoc-ref %build-inputs "htslib") "/bin/bgzip") (string-append "TABIX=" (assoc-ref %build-inputs "htslib") "/bin/tabix")) #:phases (modify-phases %standard-phases (add-after 'unpack 'patch-Makefile (lambda _ (substitute* "Makefile" ;; Do not attempt to build htslib. (("^include \\$\\(HTSDIR\\)/htslib\\.mk") "") ;; Link against GSL cblas. (("-lcblas") "-lgslcblas")) #t)) (delete 'configure) (add-before 'check 'patch-tests (lambda _ (substitute* "test/test.pl" (("/bin/bash") (which "bash"))) #t))))) (native-inputs `(("htslib" ,htslib) ("perl" ,perl))) (inputs `(("gsl" ,gsl) ("zlib" ,zlib))) (home-page "https://samtools.github.io/bcftools/") (synopsis "Utilities for variant calling and manipulating VCFs and BCFs") (description "BCFtools is a set of utilities that manipulate variant calls in the Variant Call Format (VCF) and its binary counterpart BCF. All commands work transparently with both VCFs and BCFs, both uncompressed and BGZF-compressed.") ;; The sources are dual MIT/GPL, but becomes GPL-only when USE_GPL=1. (license (list license:gpl3+ license:expat)))) (define-public bedops (package (name "bedops") (version "2.4.14") (source (origin (method url-fetch) (uri (string-append "https://github.com/bedops/bedops/archive/v" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "1kqbac547wyqma81cyky9n7mkgikjpsfd3nnmcm6hpqwanqgh10v")))) (build-system gnu-build-system) (arguments '(#:tests? #f #:make-flags (list (string-append "BINDIR=" %output "/bin")) #:phases (alist-cons-after 'unpack 'unpack-tarballs (lambda _ ;; FIXME: Bedops includes tarballs of minimally patched upstream ;; libraries jansson, zlib, and bzip2. We cannot just use stock ;; libraries because at least one of the libraries (zlib) is ;; patched to add a C++ function definition (deflateInit2cpp). ;; Until the Bedops developers offer a way to link against system ;; libraries we have to build the in-tree copies of these three ;; libraries. ;; See upstream discussion: ;; https://github.com/bedops/bedops/issues/124 ;; Unpack the tarballs to benefit from shebang patching. (with-directory-excursion "third-party" (and (zero? (system* "tar" "xvf" "jansson-2.6.tar.bz2")) (zero? (system* "tar" "xvf" "zlib-1.2.7.tar.bz2")) (zero? (system* "tar" "xvf" "bzip2-1.0.6.tar.bz2")))) ;; Disable unpacking of tarballs in Makefile. (substitute* "system.mk/Makefile.linux" (("^\tbzcat .*") "\t@echo \"not unpacking\"\n") (("\\./configure") "CONFIG_SHELL=bash ./configure")) (substitute* "third-party/zlib-1.2.7/Makefile.in" (("^SHELL=.*$") "SHELL=bash\n"))) (alist-delete 'configure %standard-phases)))) (home-page "https://github.com/bedops/bedops") (synopsis "Tools for high-performance genomic feature operations") (description "BEDOPS is a suite of tools to address common questions raised in genomic studies---mostly with regard to overlap and proximity relationships between data sets. It aims to be scalable and flexible, facilitating the efficient and accurate analysis and management of large-scale genomic data. BEDOPS provides tools that perform highly efficient and scalable Boolean and other set operations, statistical calculations, archiving, conversion and other management of genomic data of arbitrary scale. Tasks can be easily split by chromosome for distributing whole-genome analyses across a computational cluster.") (license license:gpl2+))) (define-public bedtools (package (name "bedtools") (version "2.26.0") (source (origin (method url-fetch) (uri (string-append "https://github.com/arq5x/bedtools2/archive/v" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "0xvri5hnp2iim1cx6mcd5d9f102p5ql41x69rd6106x1c17pinqm")))) (build-system gnu-build-system) (native-inputs `(("python" ,python-2))) (inputs `(("samtools" ,samtools) ("zlib" ,zlib))) (arguments '(#:test-target "test" #:phases (modify-phases %standard-phases (delete 'configure) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin/"))) (for-each (lambda (file) (install-file file bin)) (find-files "bin" ".*"))) #t))))) (home-page "https://github.com/arq5x/bedtools2") (synopsis "Tools for genome analysis and arithmetic") (description "Collectively, the bedtools utilities are a swiss-army knife of tools for a wide-range of genomics analysis tasks. The most widely-used tools enable genome arithmetic: that is, set theory on the genome. For example, bedtools allows one to intersect, merge, count, complement, and shuffle genomic intervals from multiple files in widely-used genomic file formats such as BAM, BED, GFF/GTF, VCF.") (license license:gpl2))) ;; Later releases of bedtools produce files with more columns than ;; what Ribotaper expects. (define-public bedtools-2.18 (package (inherit bedtools) (name "bedtools") (version "2.18.0") (source (origin (method url-fetch) (uri (string-append "https://github.com/arq5x/bedtools2/" "archive/v" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "05vrnr8yp7swfagshzpgqmzk1blnwnq8pq5pckzi1m26w98d63vf")))))) (define-public ribotaper (package (name "ribotaper") (version "1.3.1") (source (origin (method url-fetch) (uri (string-append "https://ohlerlab.mdc-berlin.de/" "files/RiboTaper/RiboTaper_Version_" version ".tar.gz")) (sha256 (base32 "0ykjbps1y3z3085q94npw8i9x5gldc6shy8vlc08v76zljsm07hv")))) (build-system gnu-build-system) (inputs `(("bedtools" ,bedtools-2.18) ("samtools" ,samtools-0.1) ("r" ,r) ("r-foreach" ,r-foreach) ("r-xnomial" ,r-xnomial) ("r-domc" ,r-domc) ("r-multitaper" ,r-multitaper) ("r-seqinr" ,r-seqinr))) (home-page "https://ohlerlab.mdc-berlin.de/software/RiboTaper_126/") (synopsis "Define translated ORFs using ribosome profiling data") (description "Ribotaper is a method for defining translated @dfn{open reading frames} (ORFs) using ribosome profiling (ribo-seq) data. This package provides the Ribotaper pipeline.") (license license:gpl3+))) (define-public bioawk (package (name "bioawk") (version "1.0") (source (origin (method url-fetch) (uri (string-append "https://github.com/lh3/bioawk/archive/v" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "1daizxsk17ahi9n58fj8vpgwyhzrzh54bzqhanjanp88kgrz7gjw")))) (build-system gnu-build-system) (inputs `(("zlib" ,zlib))) (native-inputs `(("bison" ,bison))) (arguments `(#:tests? #f ; There are no tests to run. ;; Bison must generate files, before other targets can build. #:parallel-build? #f #:phases (modify-phases %standard-phases (delete 'configure) ; There is no configure phase. (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin")) (man (string-append out "/share/man/man1"))) (mkdir-p man) (copy-file "awk.1" (string-append man "/bioawk.1")) (install-file "bioawk" bin))))))) (home-page "https://github.com/lh3/bioawk") (synopsis "AWK with bioinformatics extensions") (description "Bioawk is an extension to Brian Kernighan's awk, adding the support of several common biological data formats, including optionally gzip'ed BED, GFF, SAM, VCF, FASTA/Q and TAB-delimited formats with column names. It also adds a few built-in functions and a command line option to use TAB as the input/output delimiter. When the new functionality is not used, bioawk is intended to behave exactly the same as the original BWK awk.") (license license:x11))) (define-public python2-pybedtools (package (name "python2-pybedtools") (version "0.6.9") (source (origin (method url-fetch) (uri (string-append "https://pypi.python.org/packages/source/p/pybedtools/pybedtools-" version ".tar.gz")) (sha256 (base32 "1ldzdxw1p4y3g2ignmggsdypvqkcwqwzhdha4rbgpih048z5p4an")))) (build-system python-build-system) (arguments `(#:python ,python-2)) ; no Python 3 support (inputs `(("python-cython" ,python2-cython) ("python-matplotlib" ,python2-matplotlib))) (propagated-inputs `(("bedtools" ,bedtools) ("samtools" ,samtools))) (native-inputs `(("python-pyyaml" ,python2-pyyaml) ("python-nose" ,python2-nose) ("python-setuptools" ,python2-setuptools))) (home-page "https://pythonhosted.org/pybedtools/") (synopsis "Python wrapper for BEDtools programs") (description "pybedtools is a Python wrapper for Aaron Quinlan's BEDtools programs, which are widely used for genomic interval manipulation or \"genome algebra\". pybedtools extends BEDTools by offering feature-level manipulations from with Python.") (license license:gpl2+))) (define-public python-biom-format (package (name "python-biom-format") (version "2.1.5") (source (origin (method url-fetch) ;; Use GitHub as source because PyPI distribution does not contain ;; test data: https://github.com/biocore/biom-format/issues/693 (uri (string-append "https://github.com/biocore/biom-format/archive/" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "1n25w3p1rixbpac8iysmzcja6m4ip5r6sz19l8y6wlwi49hxn278")))) (build-system python-build-system) (propagated-inputs `(("python-numpy" ,python-numpy) ("python-scipy" ,python-scipy) ("python-future" ,python-future) ("python-click" ,python-click) ("python-h5py" ,python-h5py))) (home-page "http://www.biom-format.org") (synopsis "Biological Observation Matrix (BIOM) format utilities") (description "The BIOM file format is designed to be a general-use format for representing counts of observations e.g. operational taxonomic units, KEGG orthology groups or lipid types, in one or more biological samples e.g. microbiome samples, genomes, metagenomes.") (license license:bsd-3) (properties `((python2-variant . ,(delay python2-biom-format)))))) (define-public python2-biom-format (let ((base (package-with-python2 (strip-python2-variant python-biom-format)))) (package (inherit base) (arguments `(#:phases (modify-phases %standard-phases ;; Do not require the unmaintained pyqi library. (add-after 'unpack 'remove-pyqi (lambda _ (substitute* "setup.py" (("install_requires.append\\(\"pyqi\"\\)") "pass")) #t))) ,@(package-arguments base))) (native-inputs `(("python2-setuptools" ,python2-setuptools) ,@(package-native-inputs base)))))) (define-public bioperl-minimal (let* ((inputs `(("perl-module-build" ,perl-module-build) ("perl-data-stag" ,perl-data-stag) ("perl-libwww" ,perl-libwww) ("perl-uri" ,perl-uri))) (transitive-inputs (map (compose package-name cadr) (delete-duplicates (concatenate (map (compose package-transitive-target-inputs cadr) inputs)))))) (package (name "bioperl-minimal") (version "1.7.0") (source (origin (method url-fetch) (uri (string-append "https://github.com/bioperl/bioperl-live/" "archive/release-" (string-map (lambda (c) (if (char=? c #\.) #\- c)) version) ".tar.gz")) (sha256 (base32 "12phgpxwgkqflkwfb9dcqg7a31dpjlfhar8wcgv0aj5ln4akfz06")))) (build-system perl-build-system) (arguments `(#:phases (modify-phases %standard-phases (add-after 'install 'wrap-programs (lambda* (#:key outputs #:allow-other-keys) ;; Make sure all executables in "bin" find the required Perl ;; modules at runtime. As the PERL5LIB variable contains also ;; the paths of native inputs, we pick the transitive target ;; inputs from %build-inputs. (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin/")) (path (string-join (cons (string-append out "/lib/perl5/site_perl") (map (lambda (name) (assoc-ref %build-inputs name)) ',transitive-inputs)) ":"))) (for-each (lambda (file) (wrap-program file `("PERL5LIB" ":" prefix (,path)))) (find-files bin "\\.pl$")) #t)))))) (inputs inputs) (native-inputs `(("perl-test-most" ,perl-test-most))) (home-page "http://search.cpan.org/dist/BioPerl") (synopsis "Bioinformatics toolkit") (description "BioPerl is the product of a community effort to produce Perl code which is useful in biology. Examples include Sequence objects, Alignment objects and database searching objects. These objects not only do what they are advertised to do in the documentation, but they also interact - Alignment objects are made from the Sequence objects, Sequence objects have access to Annotation and SeqFeature objects and databases, Blast objects can be converted to Alignment objects, and so on. This means that the objects provide a coordinated and extensible framework to do computational biology.") (license (package-license perl))))) (define-public python-biopython (package (name "python-biopython") (version "1.68") (source (origin (method url-fetch) ;; use PyPi rather than biopython.org to ease updating (uri (pypi-uri "biopython" version)) (sha256 (base32 "07qc7nz0k77y8hf8s18rscvibvm91zw0kkq7ylrhisf8vp8hkp6i")))) (build-system python-build-system) (arguments `(#:phases (modify-phases %standard-phases (add-before 'check 'set-home ;; Some tests require a home directory to be set. (lambda _ (setenv "HOME" "/tmp") #t))))) (inputs `(("python-numpy" ,python-numpy))) (home-page "http://biopython.org/") (synopsis "Tools for biological computation in Python") (description "Biopython is a set of tools for biological computation including parsers for bioinformatics files into Python data structures; interfaces to common bioinformatics programs; a standard sequence class and tools for performing common operations on them; code to perform data classification; code for dealing with alignments; code making it easy to split up parallelizable tasks into separate processes; and more.") (license (license:non-copyleft "http://www.biopython.org/DIST/LICENSE")) (properties `((python2-variant . ,(delay python2-biopython)))))) (define-public python2-biopython (let ((base (package-with-python2 (strip-python2-variant python-biopython)))) (package (inherit base) (native-inputs `(("python2-setuptools" ,python2-setuptools) ,@(package-native-inputs base)))))) ;; An outdated version of biopython is required for seqmagick, see ;; https://github.com/fhcrc/seqmagick/issues/59 ;; When that issue has been resolved this package should be removed. (define python2-biopython-1.66 (package (inherit python2-biopython) (version "1.66") (source (origin (method url-fetch) (uri (pypi-uri "biopython" version)) (sha256 (base32 "1gdv92593klimg22icf5j9by7xiq86jnwzkpz4abaa05ylkdf6hp")))))) (define-public bpp-core ;; The last release was in 2014 and the recommended way to install from source ;; is to clone the git repository, so we do this. ;; http://biopp.univ-montp2.fr/wiki/index.php/Main_Page (let ((commit "7d8bced0d1a87291ea8dd7046b7fb5ff9c35c582")) (package (name "bpp-core") (version (string-append "2.2.0-1." (string-take commit 7))) (source (origin (method git-fetch) (uri (git-reference (url "http://biopp.univ-montp2.fr/git/bpp-core") (commit commit))) (file-name (string-append name "-" version "-checkout")) (sha256 (base32 "10djsq5vlnkilv436gnmh4irpk49v29pa69r6xiryg32xmvn909j")))) (build-system cmake-build-system) (arguments `(#:parallel-build? #f)) (inputs `(("gcc" ,gcc-5))) ; Compilation of bpp-phyl fails with GCC 4.9 so we ; compile all of the bpp packages with GCC 5. (home-page "http://biopp.univ-montp2.fr") (synopsis "C++ libraries for Bioinformatics") (description "Bio++ is a set of C++ libraries for Bioinformatics, including sequence analysis, phylogenetics, molecular evolution and population genetics. It is Object Oriented and is designed to be both easy to use and computer efficient. Bio++ intends to help programmers to write computer expensive programs, by providing them a set of re-usable tools.") (license license:cecill-c)))) (define-public bpp-phyl ;; The last release was in 2014 and the recommended way to install from source ;; is to clone the git repository, so we do this. ;; http://biopp.univ-montp2.fr/wiki/index.php/Main_Page (let ((commit "0c07167b629f68b569bf274d1ad0c4af83276ae2")) (package (name "bpp-phyl") (version (string-append "2.2.0-1." (string-take commit 7))) (source (origin (method git-fetch) (uri (git-reference (url "http://biopp.univ-montp2.fr/git/bpp-phyl") (commit commit))) (file-name (string-append name "-" version "-checkout")) (sha256 (base32 "1ssjgchzwj3iai26kyly7gwkdv8sk59nqhkb1wpap3sf5m6kyllh")))) (build-system cmake-build-system) (arguments `(#:parallel-build? #f ;; If out-of-source, test data is not copied into the build directory ;; so the tests fail. #:out-of-source? #f)) (inputs `(("bpp-core" ,bpp-core) ("bpp-seq" ,bpp-seq) ;; GCC 4.8 fails due to an 'internal compiler error', so we use a more ;; modern GCC. ("gcc" ,gcc-5))) (home-page "http://biopp.univ-montp2.fr") (synopsis "Bio++ phylogenetic Library") (description "Bio++ is a set of C++ libraries for Bioinformatics, including sequence analysis, phylogenetics, molecular evolution and population genetics. This library provides phylogenetics-related modules.") (license license:cecill-c)))) (define-public bpp-popgen ;; The last release was in 2014 and the recommended way to install from source ;; is to clone the git repository, so we do this. ;; http://biopp.univ-montp2.fr/wiki/index.php/Main_Page (let ((commit "e472bac9b1a148803895d747cd6d0c5904f85d9f")) (package (name "bpp-popgen") (version (string-append "2.2.0-1." (string-take commit 7))) (source (origin (method git-fetch) (uri (git-reference (url "http://biopp.univ-montp2.fr/git/bpp-popgen") (commit commit))) (file-name (string-append name "-" version "-checkout")) (sha256 (base32 "0yn82dzn1n5629nzja68xfrhi655709rjanyryb36vzkmymy6dw5")))) (build-system cmake-build-system) (arguments `(#:parallel-build? #f #:tests? #f)) ; There are no tests. (inputs `(("bpp-core" ,bpp-core) ("bpp-seq" ,bpp-seq) ("gcc" ,gcc-5))) (home-page "http://biopp.univ-montp2.fr") (synopsis "Bio++ population genetics library") (description "Bio++ is a set of C++ libraries for Bioinformatics, including sequence analysis, phylogenetics, molecular evolution and population genetics. This library provides population genetics-related modules.") (license license:cecill-c)))) (define-public bpp-seq ;; The last release was in 2014 and the recommended way to install from source ;; is to clone the git repository, so we do this. ;; http://biopp.univ-montp2.fr/wiki/index.php/Main_Page (let ((commit "6cfa07965ce152e5598a89df2fa80a75973bfa33")) (package (name "bpp-seq") (version (string-append "2.2.0-1." (string-take commit 7))) (source (origin (method git-fetch) (uri (git-reference (url "http://biopp.univ-montp2.fr/git/bpp-seq") (commit commit))) (file-name (string-append name "-" version "-checkout")) (sha256 (base32 "1nys5jq7jqvdg40d91wsmj3q2yzy4276cp7sp44n67p468f27zf2")))) (build-system cmake-build-system) (arguments `(#:parallel-build? #f ;; If out-of-source, test data is not copied into the build directory ;; so the tests fail. #:out-of-source? #f)) (inputs `(("bpp-core" ,bpp-core) ("gcc" ,gcc-5))) ; Use GCC 5 as per 'bpp-core'. (home-page "http://biopp.univ-montp2.fr") (synopsis "Bio++ sequence library") (description "Bio++ is a set of C++ libraries for Bioinformatics, including sequence analysis, phylogenetics, molecular evolution and population genetics. This library provides sequence-related modules.") (license license:cecill-c)))) (define-public bppsuite ;; The last release was in 2014 and the recommended way to install from source ;; is to clone the git repository, so we do this. ;; http://biopp.univ-montp2.fr/wiki/index.php/Main_Page (let ((commit "c516147f57aa50961121cd505bed52cd7603698b")) (package (name "bppsuite") (version (string-append "2.2.0-1." (string-take commit 7))) (source (origin (method git-fetch) (uri (git-reference (url "http://biopp.univ-montp2.fr/git/bppsuite") (commit commit))) (file-name (string-append name "-" version "-checkout")) (sha256 (base32 "1y87pxvw0jxjizhq2dr9g2r91md45k1p9ih2sl1yy1y3p934l2kb")))) (build-system cmake-build-system) (arguments `(#:parallel-build? #f #:tests? #f)) ; There are no tests. (native-inputs `(("groff" ,groff) ("man-db" ,man-db) ("texinfo" ,texinfo))) (inputs `(("bpp-core" ,bpp-core) ("bpp-seq" ,bpp-seq) ("bpp-phyl" ,bpp-phyl) ("bpp-phyl" ,bpp-popgen) ("gcc" ,gcc-5))) (home-page "http://biopp.univ-montp2.fr") (synopsis "Bioinformatics tools written with the Bio++ libraries") (description "Bio++ is a set of C++ libraries for Bioinformatics, including sequence analysis, phylogenetics, molecular evolution and population genetics. This package provides command line tools using the Bio++ library.") (license license:cecill-c)))) (define-public blast+ (package (name "blast+") (version "2.4.0") (source (origin (method url-fetch) (uri (string-append "ftp://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/" version "/ncbi-blast-" version "+-src.tar.gz")) (sha256 (base32 "14n9jik6vhiwjd3m7bach4xj1pzfn0szbsbyfxybd9l9cc43b6mb")) (modules '((guix build utils))) (snippet '(begin ;; Remove bundled bzip2 and zlib (delete-file-recursively "c++/src/util/compress/bzip2") (delete-file-recursively "c++/src/util/compress/zlib") (substitute* "c++/src/util/compress/Makefile.in" (("bzip2 zlib api") "api")) ;; Remove useless msbuild directory (delete-file-recursively "c++/src/build-system/project_tree_builder/msbuild") #t)))) (build-system gnu-build-system) (arguments `(;; There are three(!) tests for this massive library, and all fail with ;; "unparsable timing stats". ;; ERR [127] -- [util/regexp] test_pcre.sh (unparsable timing stats) ;; ERR [127] -- [serial/datatool] datatool.sh (unparsable timing stats) ;; ERR [127] -- [serial/datatool] datatool_xml.sh (unparsable timing stats) #:tests? #f #:out-of-source? #t #:parallel-build? #f ; not supported #:phases (modify-phases %standard-phases (add-before 'configure 'set-HOME ;; $HOME needs to be set at some point during the configure phase (lambda _ (setenv "HOME" "/tmp") #t)) (add-after 'unpack 'enter-dir (lambda _ (chdir "c++") #t)) (add-after 'enter-dir 'fix-build-system (lambda _ (define (which* cmd) (cond ((string=? cmd "date") ;; make call to "date" deterministic "date -d @0") ((which cmd) => identity) (else (format (current-error-port) "WARNING: Unable to find absolute path for ~s~%" cmd) #f))) ;; Rewrite hardcoded paths to various tools (substitute* (append '("src/build-system/configure.ac" "src/build-system/configure" "scripts/common/impl/if_diff.sh" "scripts/common/impl/run_with_lock.sh" "src/build-system/Makefile.configurables.real" "src/build-system/Makefile.in.top" "src/build-system/Makefile.meta.gmake=no" "src/build-system/Makefile.meta.in" "src/build-system/Makefile.meta_l" "src/build-system/Makefile.meta_p" "src/build-system/Makefile.meta_r" "src/build-system/Makefile.mk.in" "src/build-system/Makefile.requirements" "src/build-system/Makefile.rules_with_autodep.in") (find-files "scripts/common/check" "\\.sh$")) (("(/usr/bin/|/bin/)([a-z][-_.a-z]*)" all dir cmd) (or (which* cmd) all))) (substitute* (find-files "src/build-system" "^config.*") (("LN_S=/bin/\\$LN_S") (string-append "LN_S=" (which "ln"))) (("^PATH=.*") "")) ;; rewrite "/var/tmp" in check script (substitute* "scripts/common/check/check_make_unix.sh" (("/var/tmp") "/tmp")) ;; do not reset PATH (substitute* (find-files "scripts/common/impl/" "\\.sh$") (("^ *PATH=.*") "") (("action=/bin/") "action=") (("export PATH") ":")) #t)) (replace 'configure (lambda* (#:key inputs outputs #:allow-other-keys) (let ((out (assoc-ref outputs "out")) (lib (string-append (assoc-ref outputs "lib") "/lib")) (include (string-append (assoc-ref outputs "include") "/include/ncbi-tools++"))) ;; The 'configure' script doesn't recognize things like ;; '--enable-fast-install'. (zero? (system* "./configure.orig" (string-append "--with-build-root=" (getcwd) "/build") (string-append "--prefix=" out) (string-append "--libdir=" lib) (string-append "--includedir=" include) (string-append "--with-bz2=" (assoc-ref inputs "bzip2")) (string-append "--with-z=" (assoc-ref inputs "zlib")) ;; Each library is built twice by default, once ;; with "-static" in its name, and again ;; without. "--without-static" "--with-dll")))))))) (outputs '("out" ; 19 MB "lib" ; 203 MB "include")) ; 32 MB (inputs `(("bzip2" ,bzip2) ("zlib" ,zlib))) (native-inputs `(("cpio" ,cpio))) (home-page "http://blast.ncbi.nlm.nih.gov") (synopsis "Basic local alignment search tool") (description "BLAST is a popular method of performing a DNA or protein sequence similarity search, using heuristics to produce results quickly. It also calculates an “expect value” that estimates how many matches would have occurred at a given score by chance, which can aid a user in judging how much confidence to have in an alignment.") ;; Most of the sources are in the public domain, with the following ;; exceptions: ;; * Expat: ;; * ./c++/include/util/bitset/ ;; * ./c++/src/html/ncbi_menu*.js ;; * Boost license: ;; * ./c++/include/util/impl/floating_point_comparison.hpp ;; * LGPL 2+: ;; * ./c++/include/dbapi/driver/odbc/unix_odbc/ ;; * ASL 2.0: ;; * ./c++/src/corelib/teamcity_* (license (list license:public-domain license:expat license:boost1.0 license:lgpl2.0+ license:asl2.0)))) (define-public bless (package (name "bless") (version "1p02") (source (origin (method url-fetch) (uri (string-append "mirror://sourceforge/bless-ec/bless.v" version ".tgz")) (sha256 (base32 "0rm0gw2s18dqwzzpl3c2x1z05ni2v0xz5dmfk3d33j6g4cgrlrdd")) (modules '((guix build utils))) (snippet `(begin ;; Remove bundled boost, pigz, zlib, and .git directory ;; FIXME: also remove bundled sources for murmurhash3 and ;; kmc once packaged. (delete-file-recursively "boost") (delete-file-recursively "pigz") (delete-file-recursively "google-sparsehash") (delete-file-recursively "zlib") (delete-file-recursively ".git") #t)))) (build-system gnu-build-system) (arguments '(#:tests? #f ;no "check" target #:make-flags (list (string-append "ZLIB=" (assoc-ref %build-inputs "zlib") "/lib/libz.a") (string-append "LDFLAGS=" (string-join '("-lboost_filesystem" "-lboost_system" "-lboost_iostreams" "-lz" "-fopenmp" "-std=c++11")))) #:phases (modify-phases %standard-phases (add-after 'unpack 'do-not-build-bundled-pigz (lambda* (#:key inputs outputs #:allow-other-keys) (substitute* "Makefile" (("cd pigz/pigz-2.3.3; make") "")) #t)) (add-after 'unpack 'patch-paths-to-executables (lambda* (#:key inputs outputs #:allow-other-keys) (substitute* "parse_args.cpp" (("kmc_binary = .*") (string-append "kmc_binary = \"" (assoc-ref outputs "out") "/bin/kmc\";")) (("pigz_binary = .*") (string-append "pigz_binary = \"" (assoc-ref inputs "pigz") "/bin/pigz\";"))) #t)) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin/"))) (for-each (lambda (file) (install-file file bin)) '("bless" "kmc/bin/kmc")) #t))) (delete 'configure)))) (native-inputs `(("perl" ,perl))) (inputs `(("openmpi" ,openmpi) ("boost" ,boost) ("sparsehash" ,sparsehash) ("pigz" ,pigz) ("zlib" ,zlib))) (supported-systems '("x86_64-linux")) (home-page "http://sourceforge.net/p/bless-ec/wiki/Home/") (synopsis "Bloom-filter-based error correction tool for NGS reads") (description "@dfn{Bloom-filter-based error correction solution for high-throughput sequencing reads} (BLESS) uses a single minimum-sized bloom filter is a correction tool for genomic reads produced by @dfn{Next-generation sequencing} (NGS). BLESS produces accurate correction results with much less memory compared with previous solutions and is also able to tolerate a higher false-positive rate. BLESS can extend reads like DNA assemblers to correct errors at the end of reads.") (license license:gpl3+))) (define-public bowtie (package (name "bowtie") (version "2.2.9") (source (origin (method url-fetch) (uri (string-append "https://github.com/BenLangmead/bowtie2/archive/v" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "1vp5db8i7is57iwjybcdg18f5ivyzlj5g1ix1nlvxainzivhz55g")) (modules '((guix build utils))) (snippet '(substitute* "Makefile" ;; replace BUILD_HOST and BUILD_TIME for deterministic build (("-DBUILD_HOST=.*") "-DBUILD_HOST=\"\\\"guix\\\"\"") (("-DBUILD_TIME=.*") "-DBUILD_TIME=\"\\\"0\\\"\""))))) (build-system gnu-build-system) (inputs `(("perl" ,perl) ("perl-clone" ,perl-clone) ("perl-test-deep" ,perl-test-deep) ("perl-test-simple" ,perl-test-simple) ("python" ,python-2) ("tbb" ,tbb))) (arguments '(#:make-flags (list "allall" "WITH_TBB=1" (string-append "prefix=" (assoc-ref %outputs "out"))) #:phases (alist-delete 'configure (alist-replace 'check (lambda* (#:key outputs #:allow-other-keys) (system* "perl" "scripts/test/simple_tests.pl" "--bowtie2=./bowtie2" "--bowtie2-build=./bowtie2-build")) %standard-phases)))) (home-page "http://bowtie-bio.sourceforge.net/bowtie2/index.shtml") (synopsis "Fast and sensitive nucleotide sequence read aligner") (description "Bowtie 2 is a fast and memory-efficient tool for aligning sequencing reads to long reference sequences. It is particularly good at aligning reads of about 50 up to 100s or 1,000s of characters, and particularly good at aligning to relatively long (e.g. mammalian) genomes. Bowtie 2 indexes the genome with an FM Index to keep its memory footprint small: for the human genome, its memory footprint is typically around 3.2 GB. Bowtie 2 supports gapped, local, and paired-end alignment modes.") (supported-systems '("x86_64-linux")) (license license:gpl3+))) (define-public tophat (package (name "tophat") (version "2.1.0") (source (origin (method url-fetch) (uri (string-append "http://ccb.jhu.edu/software/tophat/downloads/tophat-" version ".tar.gz")) (sha256 (base32 "168zlzykq622zbgkh90a90f1bdgsxkscq2zxzbj8brq80hbjpyp7")) (patches (search-patches "tophat-build-with-later-seqan.patch")) (modules '((guix build utils))) (snippet '(begin ;; Remove bundled SeqAn and samtools (delete-file-recursively "src/SeqAn-1.3") (delete-file-recursively "src/samtools-0.1.18") #t)))) (build-system gnu-build-system) (arguments '(#:parallel-build? #f ; not supported #:phases (modify-phases %standard-phases (add-after 'unpack 'use-system-samtools (lambda* (#:key inputs #:allow-other-keys) (substitute* "src/Makefile.in" (("(noinst_LIBRARIES = )\\$\\(SAMLIB\\)" _ prefix) prefix) (("\\$\\(SAMPROG\\): \\$\\(SAMLIB\\)") "") (("SAMPROG = samtools_0\\.1\\.18") "") (("\\$\\(samtools_0_1_18_SOURCES\\)") "") (("am__EXEEXT_1 = samtools_0\\.1\\.18\\$\\(EXEEXT\\)") "")) (substitute* '("src/common.cpp" "src/tophat.py") (("samtools_0.1.18") (which "samtools"))) (substitute* '("src/common.h" "src/bam2fastx.cpp") (("#include \"bam.h\"") "#include ") (("#include \"sam.h\"") "#include ")) (substitute* '("src/bwt_map.h" "src/map2gtf.h" "src/align_status.h") (("#include ") "#include ") (("#include ") "#include ")) #t))))) (inputs `(("boost" ,boost) ("bowtie" ,bowtie) ("samtools" ,samtools-0.1) ("ncurses" ,ncurses) ("python" ,python-2) ("perl" ,perl) ("zlib" ,zlib) ("seqan" ,seqan))) (home-page "http://ccb.jhu.edu/software/tophat/index.shtml") (synopsis "Spliced read mapper for RNA-Seq data") (description "TopHat is a fast splice junction mapper for nucleotide sequence reads produced by the RNA-Seq method. It aligns RNA-Seq reads to mammalian-sized genomes using the ultra high-throughput short read aligner Bowtie, and then analyzes the mapping results to identify splice junctions between exons.") ;; TopHat is released under the Boost Software License, Version 1.0 ;; See https://github.com/infphilo/tophat/issues/11#issuecomment-121589893 (license license:boost1.0))) (define-public bwa (package (name "bwa") (version "0.7.12") (source (origin (method url-fetch) (uri (string-append "mirror://sourceforge/bio-bwa/bwa-" version ".tar.bz2")) (sha256 (base32 "1330dpqncv0px3pbhjzz1gwgg39kkcv2r9qp2xs0sixf8z8wl7bh")))) (build-system gnu-build-system) (arguments '(#:tests? #f ;no "check" target #:phases (alist-replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin")) (doc (string-append (assoc-ref outputs "out") "/share/doc/bwa")) (man (string-append (assoc-ref outputs "out") "/share/man/man1"))) (mkdir-p bin) (mkdir-p doc) (mkdir-p man) (install-file "bwa" bin) (install-file "README.md" doc) (install-file "bwa.1" man))) ;; no "configure" script (alist-delete 'configure %standard-phases)))) (inputs `(("zlib" ,zlib))) ;; Non-portable SSE instructions are used so building fails on platforms ;; other than x86_64. (supported-systems '("x86_64-linux")) (home-page "http://bio-bwa.sourceforge.net/") (synopsis "Burrows-Wheeler sequence aligner") (description "BWA is a software package for mapping low-divergent sequences against a large reference genome, such as the human genome. It consists of three algorithms: BWA-backtrack, BWA-SW and BWA-MEM. The first algorithm is designed for Illumina sequence reads up to 100bp, while the rest two for longer sequences ranged from 70bp to 1Mbp. BWA-MEM and BWA-SW share similar features such as long-read support and split alignment, but BWA-MEM, which is the latest, is generally recommended for high-quality queries as it is faster and more accurate. BWA-MEM also has better performance than BWA-backtrack for 70-100bp Illumina reads.") (license license:gpl3+))) (define-public bwa-pssm (package (inherit bwa) (name "bwa-pssm") (version "0.5.11") (source (origin (method url-fetch) (uri (string-append "https://github.com/pkerpedjiev/bwa-pssm/" "archive/" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "02p7mpbs4mlxmn84g2x4ghak638vbj4lqix2ipx5g84pz9bhdavg")))) (build-system gnu-build-system) (inputs `(("gdsl" ,gdsl) ("zlib" ,zlib) ("perl" ,perl))) (home-page "http://bwa-pssm.binf.ku.dk/") (synopsis "Burrows-Wheeler transform-based probabilistic short read mapper") (description "BWA-PSSM is a probabilistic short genomic sequence read aligner based on the use of @dfn{position specific scoring matrices} (PSSM). Like many of the existing aligners it is fast and sensitive. Unlike most other aligners, however, it is also adaptible in the sense that one can direct the alignment based on known biases within the data set. It is coded as a modification of the original BWA alignment program and shares the genome index structure as well as many of the command line options.") (license license:gpl3+))) (define-public python2-bx-python (package (name "python2-bx-python") (version "0.7.2") (source (origin (method url-fetch) (uri (string-append "https://pypi.python.org/packages/source/b/bx-python/bx-python-" version ".tar.gz")) (sha256 (base32 "0ld49idhc5zjdvbhvjq1a2qmpjj7h5v58rqr25dzmfq7g34b50xh")) (modules '((guix build utils))) (snippet '(substitute* "setup.py" ;; remove dependency on outdated "distribute" module (("^from distribute_setup import use_setuptools") "") (("^use_setuptools\\(\\)") ""))))) (build-system python-build-system) (arguments `(#:tests? #f ;tests fail because test data are not included #:python ,python-2)) (inputs `(("python-numpy" ,python2-numpy) ("zlib" ,zlib))) (native-inputs `(("python-nose" ,python2-nose) ("python-setuptools" ,python2-setuptools))) (home-page "http://bitbucket.org/james_taylor/bx-python/") (synopsis "Tools for manipulating biological data") (description "bx-python provides tools for manipulating biological data, particularly multiple sequence alignments.") (license license:expat))) (define-public python-pysam (package (name "python-pysam") (version "0.9.1.4") (source (origin (method url-fetch) ;; Test data is missing on PyPi. (uri (string-append "https://github.com/pysam-developers/pysam/archive/v" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "0y41ssbg6nvn2jgcbnrvkzblpjcwszaiv1rgyd8dwzjkrbfsgsmc")) (modules '((guix build utils))) (snippet ;; Drop bundled htslib. TODO: Also remove samtools and bcftools. '(delete-file-recursively "htslib")))) (build-system python-build-system) (arguments `(#:phases (modify-phases %standard-phases (add-before 'build 'set-flags (lambda* (#:key inputs #:allow-other-keys) (setenv "HTSLIB_MODE" "external") (setenv "HTSLIB_LIBRARY_DIR" (string-append (assoc-ref inputs "htslib") "/lib")) (setenv "HTSLIB_INCLUDE_DIR" (string-append (assoc-ref inputs "htslib") "/include")) (setenv "LDFLAGS" "-lncurses") (setenv "CFLAGS" "-D_CURSES_LIB=1") #t)) (delete 'check) (add-after 'install 'check (lambda* (#:key inputs outputs #:allow-other-keys) (setenv "PYTHONPATH" (string-append (getenv "PYTHONPATH") ":" (assoc-ref outputs "out") "/lib/python" (string-take (string-take-right (assoc-ref inputs "python") 5) 3) "/site-packages")) ;; Step out of source dir so python does not import from CWD. (chdir "tests") (setenv "HOME" "/tmp") (and (zero? (system* "make" "-C" "pysam_data")) (zero? (system* "make" "-C" "cbcf_data")) (zero? (system* "nosetests" "-v")))))))) (propagated-inputs `(("htslib" ,htslib))) ; Included from installed header files. (inputs `(("ncurses" ,ncurses) ("zlib" ,zlib))) (native-inputs `(("python-cython" ,python-cython) ("python-setuptools" ,python-setuptools) ;; Dependencies below are are for tests only. ("samtools" ,samtools) ("bcftools" ,bcftools) ("python-nose" ,python-nose))) (home-page "https://github.com/pysam-developers/pysam") (synopsis "Python bindings to the SAMtools C API") (description "Pysam is a Python module for reading and manipulating files in the SAM/BAM format. Pysam is a lightweight wrapper of the SAMtools C API. It also includes an interface for tabix.") (license license:expat))) (define-public python2-pysam (package-with-python2 python-pysam)) (define-public python-twobitreader (package (name "python-twobitreader") (version "3.1.4") (source (origin (method url-fetch) (uri (pypi-uri "twobitreader" version)) (sha256 (base32 "1q8wnj2kga9nz1lwc4w7qv52smfm536hp6mc8w6s53lhyj0mpi22")))) (properties `((python2-variant . ,(delay python2-twobitreader)))) (build-system python-build-system) (arguments '(;; Tests are not distributed in the PyPi release. ;; TODO Try building from the Git repo or asking the upstream maintainer ;; to distribute the tests on PyPi. #:tests? #f)) (native-inputs `(("python-sphinx" ,python-sphinx))) (home-page "https://github.com/benjschiller/twobitreader") (synopsis "Python library for reading .2bit files") (description "twobitreader is a Python library for reading .2bit files as used by the UCSC genome browser.") (license license:artistic2.0))) (define-public python2-twobitreader (let ((base (package-with-python2 (strip-python2-variant python-twobitreader)))) (package (inherit base) (native-inputs `(("python2-setuptools" ,python2-setuptools) ,@(package-native-inputs base)))))) (define-public python-plastid (package (name "python-plastid") (version "0.4.6") (source (origin (method url-fetch) (uri (pypi-uri "plastid" version)) (sha256 (base32 "1sqkz5d3b9kf688mp7k771c87ins42j7j0whmkb49cb3fsg8s8lj")))) (properties `((python2-variant . ,(delay python2-plastid)))) (build-system python-build-system) (arguments ;; Some test files are not included. `(#:tests? #f)) (propagated-inputs `(("python-numpy" ,python-numpy) ("python-scipy" ,python-scipy) ("python-pandas" ,python-pandas) ("python-pysam" ,python-pysam) ("python-matplotlib" ,python-matplotlib) ("python-biopython" ,python-biopython) ("python-twobitreader" ,python-twobitreader) ("python-termcolor" ,python-termcolor))) (native-inputs `(("python-cython" ,python-cython) ("python-nose" ,python-nose))) (home-page "https://github.com/joshuagryphon/plastid") (synopsis "Python library for genomic analysis") (description "plastid is a Python library for genomic analysis – in particular, high-throughput sequencing data – with an emphasis on simplicity.") (license license:bsd-3))) (define-public python2-plastid (let ((base (package-with-python2 (strip-python2-variant python-plastid)))) (package (inherit base) ;; setuptools is required at runtime (propagated-inputs `(("python2-setuptools" ,python2-setuptools) ,@(package-propagated-inputs base)))))) (define-public cd-hit (package (name "cd-hit") (version "4.6.5") (source (origin (method url-fetch) (uri (string-append "https://github.com/weizhongli/cdhit" "/releases/download/V" version "/cd-hit-v" version "-2016-0304.tar.gz")) (sha256 (base32 "15db0hq38yyifwqx9b6l34z14jcq576dmjavhj8a426c18lvnhp3")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; there are no tests #:make-flags ;; Executables are copied directly to the PREFIX. (list (string-append "PREFIX=" (assoc-ref %outputs "out") "/bin")) #:phases (modify-phases %standard-phases ;; No "configure" script (delete 'configure) ;; Remove sources of non-determinism (add-after 'unpack 'be-timeless (lambda _ (substitute* "cdhit-utility.c++" ((" \\(built on \" __DATE__ \"\\)") "")) (substitute* "cdhit-common.c++" (("__DATE__") "\"0\"") (("\", %s, \" __TIME__ \"\\\\n\", date") "")) #t)) ;; The "install" target does not create the target directory (add-before 'install 'create-target-dir (lambda* (#:key outputs #:allow-other-keys) (mkdir-p (string-append (assoc-ref outputs "out") "/bin")) #t))))) (inputs `(("perl" ,perl))) (home-page "http://weizhongli-lab.org/cd-hit/") (synopsis "Cluster and compare protein or nucleotide sequences") (description "CD-HIT is a program for clustering and comparing protein or nucleotide sequences. CD-HIT is designed to be fast and handle extremely large databases.") ;; The manual says: "It can be copied under the GNU General Public License ;; version 2 (GPLv2)." (license license:gpl2))) (define-public clipper (package (name "clipper") (version "1.1") (source (origin (method url-fetch) (uri (string-append "https://github.com/YeoLab/clipper/archive/" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "0pflmsvhbf8izbgwhbhj1i7349sw1f55qpqj8ljmapp16hb0p0qi")) (modules '((guix build utils))) (snippet '(begin ;; remove unnecessary setup dependency (substitute* "setup.py" (("setup_requires = .*") "")) (for-each delete-file '("clipper/src/peaks.so" "clipper/src/readsToWiggle.so")) (delete-file-recursively "dist/") #t)))) (build-system python-build-system) (arguments `(#:python ,python-2)) ; only Python 2 is supported (inputs `(("htseq" ,htseq) ("python-pybedtools" ,python2-pybedtools) ("python-cython" ,python2-cython) ("python-scikit-learn" ,python2-scikit-learn) ("python-matplotlib" ,python2-matplotlib) ("python-pandas" ,python2-pandas) ("python-pysam" ,python2-pysam) ("python-numpy" ,python2-numpy) ("python-scipy" ,python2-scipy))) (native-inputs `(("python-mock" ,python2-mock) ; for tests ("python-pytz" ,python2-pytz) ; for tests ("python-setuptools" ,python2-setuptools))) (home-page "https://github.com/YeoLab/clipper") (synopsis "CLIP peak enrichment recognition") (description "CLIPper is a tool to define peaks in CLIP-seq datasets.") (license license:gpl2))) (define-public codingquarry (package (name "codingquarry") (version "2.0") (source (origin (method url-fetch) (uri (string-append "mirror://sourceforge/codingquarry/CodingQuarry_v" version ".tar.gz")) (sha256 (base32 "0115hkjflsnfzn36xppwf9h9avfxlavr43djqmshkkzbgjzsz60i")))) (build-system gnu-build-system) (arguments '(#:tests? #f ; no "check" target #:phases (modify-phases %standard-phases (delete 'configure) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin")) (doc (string-append out "/share/doc/codingquarry"))) (install-file "INSTRUCTIONS.pdf" doc) (copy-recursively "QuarryFiles" (string-append out "/QuarryFiles")) (install-file "CodingQuarry" bin) (install-file "CufflinksGTF_to_CodingQuarryGFF3.py" bin))))))) (inputs `(("openmpi" ,openmpi))) (native-search-paths (list (search-path-specification (variable "QUARRY_PATH") (files '("QuarryFiles"))))) (native-inputs `(("python" ,python-2))) ; Only Python 2 is supported (synopsis "Fungal gene predictor") (description "CodingQuarry is a highly accurate, self-training GHMM fungal gene predictor designed to work with assembled, aligned RNA-seq transcripts.") (home-page "https://sourceforge.net/projects/codingquarry/") (license license:gpl3+))) (define-public couger (package (name "couger") (version "1.8.2") (source (origin (method url-fetch) (uri (string-append "http://couger.oit.duke.edu/static/assets/COUGER" version ".zip")) (sha256 (base32 "04p2b14nmhzxw5h72mpzdhalv21bx4w9b87z0wpw0xzxpysyncmq")))) (build-system gnu-build-system) (arguments `(#:tests? #f #:phases (modify-phases %standard-phases (delete 'configure) (delete 'build) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((out (assoc-ref outputs "out"))) (copy-recursively "src" (string-append out "/src")) (mkdir (string-append out "/bin")) ;; Add "src" directory to module lookup path. (substitute* "couger" (("from argparse") (string-append "import sys\nsys.path.append(\"" out "\")\nfrom argparse"))) (copy-file "couger" (string-append out "/bin/couger"))) #t)) (add-after 'install 'wrap-program (lambda* (#:key inputs outputs #:allow-other-keys) ;; Make sure 'couger' runs with the correct PYTHONPATH. (let* ((out (assoc-ref outputs "out")) (path (getenv "PYTHONPATH"))) (wrap-program (string-append out "/bin/couger") `("PYTHONPATH" ":" prefix (,path)))) #t))))) (inputs `(("python" ,python-2) ("python2-pillow" ,python2-pillow) ("python2-numpy" ,python2-numpy) ("python2-scipy" ,python2-scipy) ("python2-matplotlib" ,python2-matplotlib))) (propagated-inputs `(("r" ,r) ("libsvm" ,libsvm) ("randomjungle" ,randomjungle))) (native-inputs `(("unzip" ,unzip))) (home-page "http://couger.oit.duke.edu") (synopsis "Identify co-factors in sets of genomic regions") (description "COUGER can be applied to any two sets of genomic regions bound by paralogous TFs (e.g., regions derived from ChIP-seq experiments) to identify putative co-factors that provide specificity to each TF. The framework determines the genomic targets uniquely-bound by each TF, and identifies a small set of co-factors that best explain the in vivo binding differences between the two TFs. COUGER uses classification algorithms (support vector machines and random forests) with features that reflect the DNA binding specificities of putative co-factors. The features are generated either from high-throughput TF-DNA binding data (from protein binding microarray experiments), or from large collections of DNA motifs.") (license license:gpl3+))) (define-public clustal-omega (package (name "clustal-omega") (version "1.2.1") (source (origin (method url-fetch) (uri (string-append "http://www.clustal.org/omega/clustal-omega-" version ".tar.gz")) (sha256 (base32 "02ibkx0m0iwz8nscg998bh41gg251y56cgh86bvyrii5m8kjgwqf")))) (build-system gnu-build-system) (inputs `(("argtable" ,argtable))) (home-page "http://www.clustal.org/omega/") (synopsis "Multiple sequence aligner for protein and DNA/RNA") (description "Clustal-Omega is a general purpose multiple sequence alignment (MSA) program for protein and DNA/RNA. It produces high quality MSAs and is capable of handling data-sets of hundreds of thousands of sequences in reasonable time.") (license license:gpl2+))) (define-public crossmap (package (name "crossmap") (version "0.2.1") (source (origin (method url-fetch) (uri (string-append "mirror://sourceforge/crossmap/CrossMap-" version ".tar.gz")) (sha256 (base32 "07y179f63d7qnzdvkqcziwk9bs3k4zhp81q392fp1hwszjdvy22f")) ;; This patch has been sent upstream already and is available ;; for download from Sourceforge, but it has not been merged. (patches (search-patches "crossmap-allow-system-pysam.patch")) (modules '((guix build utils))) ;; remove bundled copy of pysam (snippet '(delete-file-recursively "lib/pysam")))) (build-system python-build-system) (arguments `(#:python ,python-2 #:phases (alist-cons-after 'unpack 'set-env (lambda _ (setenv "CROSSMAP_USE_SYSTEM_PYSAM" "1")) %standard-phases))) (inputs `(("python-numpy" ,python2-numpy) ("python-pysam" ,python2-pysam) ("zlib" ,zlib))) (native-inputs `(("python-cython" ,python2-cython) ("python-nose" ,python2-nose) ("python-setuptools" ,python2-setuptools))) (home-page "http://crossmap.sourceforge.net/") (synopsis "Convert genome coordinates between assemblies") (description "CrossMap is a program for conversion of genome coordinates or annotation files between different genome assemblies. It supports most commonly used file formats including SAM/BAM, Wiggle/BigWig, BED, GFF/GTF, VCF.") (license license:gpl2+))) (define-public cufflinks (package (name "cufflinks") (version "2.2.1") (source (origin (method url-fetch) (uri (string-append "http://cole-trapnell-lab.github.io/" "cufflinks/assets/downloads/cufflinks-" version ".tar.gz")) (sha256 (base32 "1bnm10p8m7zq4qiipjhjqb24csiqdm1pwc8c795z253r2xk6ncg8")))) (build-system gnu-build-system) (arguments `(#:make-flags (list ;; The includes for "eigen" are located in a subdirectory. (string-append "EIGEN_CPPFLAGS=" "-I" (assoc-ref %build-inputs "eigen") "/include/eigen3/") ;; Cufflinks must be linked with various boost libraries. (string-append "LDFLAGS=" (string-join '("-lboost_system" "-lboost_serialization" "-lboost_thread")))) #:phases (modify-phases %standard-phases (add-after 'unpack 'fix-search-for-bam (lambda _ (substitute* '("ax_bam.m4" "configure" "src/hits.h") (("") "") (("") "") (("") "")) #t))) #:configure-flags (list (string-append "--with-bam=" (assoc-ref %build-inputs "samtools"))))) (inputs `(("eigen" ,eigen) ("samtools" ,samtools-0.1) ("htslib" ,htslib) ("boost" ,boost) ("python" ,python-2) ("zlib" ,zlib))) (home-page "http://cole-trapnell-lab.github.io/cufflinks/") (synopsis "Transcriptome assembly and RNA-Seq expression analysis") (description "Cufflinks assembles RNA transcripts, estimates their abundances, and tests for differential expression and regulation in RNA-Seq samples. It accepts aligned RNA-Seq reads and assembles the alignments into a parsimonious set of transcripts. Cufflinks then estimates the relative abundances of these transcripts based on how many reads support each one, taking into account biases in library preparation protocols.") (license license:boost1.0))) (define-public cutadapt (package (name "cutadapt") (version "1.8") (source (origin (method url-fetch) (uri (string-append "https://github.com/marcelm/cutadapt/archive/v" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "161bp87y6gd6r5bmvjpn2b1k942i3fizfpa139f0jn6jv1wcp5h5")))) (build-system python-build-system) (arguments ;; tests must be run after install `(#:phases (alist-cons-after 'install 'check (lambda* (#:key inputs outputs #:allow-other-keys) (setenv "PYTHONPATH" (string-append (getenv "PYTHONPATH") ":" (assoc-ref outputs "out") "/lib/python" (string-take (string-take-right (assoc-ref inputs "python") 5) 3) "/site-packages")) (zero? (system* "nosetests" "-P" "tests"))) (alist-delete 'check %standard-phases)))) (native-inputs `(("python-cython" ,python-cython) ("python-nose" ,python-nose) ("python-setuptools" ,python-setuptools))) (home-page "https://code.google.com/p/cutadapt/") (synopsis "Remove adapter sequences from nucleotide sequencing reads") (description "Cutadapt finds and removes adapter sequences, primers, poly-A tails and other types of unwanted sequence from high-throughput sequencing reads.") (license license:expat))) (define-public libbigwig (package (name "libbigwig") (version "0.1.4") (source (origin (method url-fetch) (uri (string-append "https://github.com/dpryan79/libBigWig/" "archive/" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "098rjh35pi4a9q83n8wiwvyzykjqj6l8q189p1xgfw4ghywdlvw1")))) (build-system gnu-build-system) (arguments `(#:test-target "test" #:make-flags (list "CC=gcc" (string-append "prefix=" (assoc-ref %outputs "out"))) #:phases (modify-phases %standard-phases (delete 'configure) (add-before 'check 'disable-curl-test (lambda _ (substitute* "Makefile" (("./test/testRemote.*") "")) #t)) ;; This has been fixed with the upstream commit 4ff6959cd8a0, but ;; there has not yet been a release containing this change. (add-before 'install 'create-target-dirs (lambda* (#:key outputs #:allow-other-keys) (let ((out (assoc-ref outputs "out"))) (mkdir-p (string-append out "/lib")) (mkdir-p (string-append out "/include")) #t)))))) (inputs `(("zlib" ,zlib) ("curl" ,curl))) (native-inputs `(("doxygen" ,doxygen))) (home-page "https://github.com/dpryan79/libBigWig") (synopsis "C library for handling bigWig files") (description "This package provides a C library for parsing local and remote BigWig files.") (license license:expat))) (define-public python-pybigwig (package (name "python-pybigwig") (version "0.2.5") (source (origin (method url-fetch) (uri (pypi-uri "pyBigWig" version)) (sha256 (base32 "0yrpdxg3y0sny25x4w22lv1k47jzccqjmg7j4bp0hywklvp0hg7d")) (modules '((guix build utils))) (snippet '(begin ;; Delete bundled libBigWig sources (delete-file-recursively "libBigWig"))))) (build-system python-build-system) (arguments `(#:phases (modify-phases %standard-phases (add-after 'unpack 'link-with-libBigWig (lambda* (#:key inputs #:allow-other-keys) (substitute* "setup.py" (("libs=\\[") "libs=[\"BigWig\", ")) #t))))) (inputs `(("libbigwig" ,libbigwig) ("zlib" ,zlib) ("curl" ,curl))) (home-page "https://github.com/dpryan79/pyBigWig") (synopsis "Access bigWig files in Python using libBigWig") (description "This package provides Python bindings to the libBigWig library for accessing bigWig files.") (license license:expat))) (define-public python2-pybigwig (let ((pybigwig (package-with-python2 python-pybigwig))) (package (inherit pybigwig) (native-inputs `(("python-setuptools" ,python2-setuptools)))))) (define-public python-dendropy (package (name "python-dendropy") (version "4.1.0") (source (origin (method url-fetch) (uri (pypi-uri "DendroPy" version)) (sha256 (base32 "1jfz7gp18wph311w1yygbvjanb3n5mdqal439bb6myw41dwb5m63")) ;; There are two known test failures that will be fixed in the next ;; release after 4.1.0. ;; https://github.com/jeetsukumaran/DendroPy/issues/48 (patches (search-patches "python-dendropy-exclude-failing-tests.patch")))) (build-system python-build-system) (home-page "http://packages.python.org/DendroPy/") (synopsis "Library for phylogenetics and phylogenetic computing") (description "DendroPy is a library for phylogenetics and phylogenetic computing: reading, writing, simulation, processing and manipulation of phylogenetic trees (phylogenies) and characters.") (license license:bsd-3) (properties `((python2-variant . ,(delay python2-dendropy)))))) (define-public python2-dendropy (let ((base (package-with-python2 (strip-python2-variant python-dendropy)))) (package (inherit base) ;; Do not use same source as 'python-dendropy' because the patched ;; failing tests do not occur on Python 2. (source (origin (method url-fetch) (uri (pypi-uri "DendroPy" (package-version base))) (sha256 (base32 "1jfz7gp18wph311w1yygbvjanb3n5mdqal439bb6myw41dwb5m63")))) (arguments `(#:python ,python-2 #:phases (modify-phases %standard-phases (replace 'check ;; There is currently a test failure that only happens on some ;; systems, and only using "setup.py test" (lambda _ (zero? (system* "nosetests"))))))) (native-inputs `(("python2-setuptools" ,python2-setuptools) ("python2-nose" ,python2-nose) ,@(package-native-inputs base)))))) (define-public deeptools (package (name "deeptools") (version "2.1.1") (source (origin (method url-fetch) (uri (string-append "https://github.com/fidelram/deepTools/" "archive/" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "1nmfin0zjdby3vay3r4flvz94dr6qjhj41ax4yz3vx13j6wz8izd")))) (build-system python-build-system) (arguments `(#:python ,python-2)) (inputs `(("python-scipy" ,python2-scipy) ("python-numpy" ,python2-numpy) ("python-numpydoc" ,python2-numpydoc) ("python-matplotlib" ,python2-matplotlib) ("python-bx-python" ,python2-bx-python) ("python-pysam" ,python2-pysam) ("python-pybigwig" ,python2-pybigwig))) (native-inputs `(("python-mock" ,python2-mock) ;for tests ("python-pytz" ,python2-pytz) ;for tests ("python-setuptools" ,python2-setuptools))) (home-page "https://github.com/fidelram/deepTools") (synopsis "Tools for normalizing and visualizing deep-sequencing data") (description "DeepTools addresses the challenge of handling the large amounts of data that are now routinely generated from DNA sequencing centers. To do so, deepTools contains useful modules to process the mapped reads data to create coverage files in standard bedGraph and bigWig file formats. By doing so, deepTools allows the creation of normalized coverage files or the comparison between two files (for example, treatment and control). Finally, using such normalized and standardized files, multiple visualizations can be created to identify enrichments with functional annotations of the genome.") (license license:gpl3+))) (define-public diamond (package (name "diamond") (version "0.8.26") (source (origin (method url-fetch) (uri (string-append "https://github.com/bbuchfink/diamond/archive/v" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "17s38pgjih6yc2z81040pircbv60c9wr33mbcyki2rfpv8rbxlh0")))) (build-system cmake-build-system) (arguments '(#:tests? #f ; no "check" target #:phases (modify-phases %standard-phases (add-after 'unpack 'remove-native-compilation (lambda _ (substitute* "CMakeLists.txt" (("-march=native") "")) #t))))) (inputs `(("zlib" ,zlib))) (home-page "https://github.com/bbuchfink/diamond") (synopsis "Accelerated BLAST compatible local sequence aligner") (description "DIAMOND is a BLAST-compatible local aligner for mapping protein and translated DNA query sequences against a protein reference database (BLASTP and BLASTX alignment mode). The speedup over BLAST is up to 20,000 on short reads at a typical sensitivity of 90-99% relative to BLAST depending on the data and settings.") ;; diamond fails to build on other platforms ;; https://github.com/bbuchfink/diamond/issues/18 (supported-systems '("x86_64-linux")) (license (license:non-copyleft "file://src/COPYING" "See src/COPYING in the distribution.")))) (define-public discrover (package (name "discrover") (version "1.6.0") (source (origin (method url-fetch) (uri (string-append "https://github.com/maaskola/discrover/archive/" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "0rah9ja4m0rl5mldd6vag9rwrivw1zrqxssfq8qx64m7961fp68k")))) (build-system cmake-build-system) (arguments `(#:tests? #f)) ; there are no tests (inputs `(("boost" ,boost) ("cairo" ,cairo))) (native-inputs `(("texlive" ,texlive) ("imagemagick" ,imagemagick))) (home-page "http://dorina.mdc-berlin.de/public/rajewsky/discrover/") (synopsis "Discover discriminative nucleotide sequence motifs") (description "Discrover is a motif discovery method to find binding sites of nucleic acid binding proteins.") (license license:gpl3+))) (define-public eigensoft (let ((revision "1") (commit "b14d1e202e21e532536ff8004f0419cd5e259dc7")) (package (name "eigensoft") (version (string-append "6.1.2-" revision "." (string-take commit 9))) (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/DReichLab/EIG.git") (commit commit))) (file-name (string-append "eigensoft-" commit "-checkout")) (sha256 (base32 "0f5m6k2j5c16xc3xbywcs989xyc26ncy1zfzp9j9n55n9r4xcaiq")) (modules '((guix build utils))) ;; Remove pre-built binaries. (snippet '(begin (delete-file-recursively "bin") (mkdir "bin") #t)))) (build-system gnu-build-system) (arguments `(#:tests? #f ; There are no tests. #:make-flags '("CC=gcc") #:phases (modify-phases %standard-phases ;; There is no configure phase, but the Makefile is in a ;; sub-directory. (replace 'configure (lambda _ (chdir "src") ;; The link flags are incomplete. (substitute* "Makefile" (("-lgsl") "-lgsl -lm -llapack -llapacke -lpthread")) #t)) ;; The provided install target only copies executables to ;; the "bin" directory in the build root. (add-after 'install 'actually-install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin"))) (mkdir-p bin) (for-each (lambda (file) (install-file file bin)) (find-files "../bin" ".*")) #t)))))) (inputs `(("gsl" ,gsl) ("lapack" ,lapack) ("openblas" ,openblas) ("perl" ,perl) ("gfortran" ,gfortran "lib"))) (home-page "https://github.com/DReichLab/EIG") (synopsis "Tools for population genetics") (description "The EIGENSOFT package provides tools for population genetics and stratification correction. EIGENSOFT implements methods commonly used in population genetics analyses such as PCA, computation of Tracy-Widom statistics, and finding related individuals in structured populations. It comes with a built-in plotting script and supports multiple file formats and quantitative phenotypes.") ;; The license of the eigensoft tools is Expat, but since it's ;; linking with the GNU Scientific Library (GSL) the effective ;; license is the GPL. (license license:gpl3+)))) (define-public edirect (package (name "edirect") (version "4.10") (source (origin (method url-fetch) (uri (string-append "ftp://ftp.ncbi.nlm.nih.gov/entrez/entrezdirect/" "versions/2016-05-03/edirect.tar.gz")) (sha256 (base32 "15zsprak5yh8c1yrz4r1knmb5s8qcmdid4xdhkh3lqcv64l60hli")))) (build-system perl-build-system) (arguments `(#:tests? #f ;no "check" target #:phases (modify-phases %standard-phases (delete 'configure) (delete 'build) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((target (string-append (assoc-ref outputs "out") "/bin"))) (mkdir-p target) (copy-file "edirect.pl" (string-append target "/edirect.pl")) #t))) (add-after 'install 'wrap-program (lambda* (#:key inputs outputs #:allow-other-keys) ;; Make sure 'edirect.pl' finds all perl inputs at runtime. (let* ((out (assoc-ref outputs "out")) (path (getenv "PERL5LIB"))) (wrap-program (string-append out "/bin/edirect.pl") `("PERL5LIB" ":" prefix (,path))))))))) (inputs `(("perl-html-parser" ,perl-html-parser) ("perl-encode-locale" ,perl-encode-locale) ("perl-file-listing" ,perl-file-listing) ("perl-html-tagset" ,perl-html-tagset) ("perl-html-tree" ,perl-html-tree) ("perl-http-cookies" ,perl-http-cookies) ("perl-http-date" ,perl-http-date) ("perl-http-message" ,perl-http-message) ("perl-http-negotiate" ,perl-http-negotiate) ("perl-lwp-mediatypes" ,perl-lwp-mediatypes) ("perl-lwp-protocol-https" ,perl-lwp-protocol-https) ("perl-net-http" ,perl-net-http) ("perl-uri" ,perl-uri) ("perl-www-robotrules" ,perl-www-robotrules) ("perl" ,perl))) (home-page "http://www.ncbi.nlm.nih.gov/books/NBK179288/") (synopsis "Tools for accessing the NCBI's set of databases") (description "Entrez Direct (EDirect) is a method for accessing the National Center for Biotechnology Information's (NCBI) set of interconnected databases (publication, sequence, structure, gene, variation, expression, etc.) from a terminal. Functions take search terms from command-line arguments. Individual operations are combined to build multi-step queries. Record retrieval and formatting normally complete the process. EDirect also provides an argument-driven function that simplifies the extraction of data from document summaries or other results that are returned in structured XML format. This can eliminate the need for writing custom software to answer ad hoc questions.") (license license:public-domain))) (define-public exonerate (package (name "exonerate") (version "2.4.0") (source (origin (method url-fetch) (uri (string-append "http://ftp.ebi.ac.uk/pub/software/vertebrategenomics/exonerate/" "exonerate-" version ".tar.gz")) (sha256 (base32 "0hj0m9xygiqsdxvbg79wq579kbrx1mdrabi2bzqz2zn9qwfjcjgq")))) (build-system gnu-build-system) (arguments `(#:parallel-build? #f)) ; Building in parallel fails on some machines. (native-inputs `(("pkg-config" ,pkg-config))) (inputs `(("glib" ,glib))) (home-page "https://www.ebi.ac.uk/about/vertebrate-genomics/software/exonerate") (synopsis "Generic tool for biological sequence alignment") (description "Exonerate is a generic tool for pairwise sequence comparison. It allows the alignment of sequences using a many alignment models, either exhaustive dynamic programming or a variety of heuristics.") (license license:gpl3))) (define-public express (package (name "express") (version "1.5.1") (source (origin (method url-fetch) (uri (string-append "http://bio.math.berkeley.edu/eXpress/downloads/express-" version "/express-" version "-src.tgz")) (sha256 (base32 "03rczxd0gjp2l1jxcmjfmf5j94j77zqyxa6x063zsc585nj40n0c")))) (build-system cmake-build-system) (arguments `(#:tests? #f ;no "check" target #:phases (alist-cons-after 'unpack 'use-shared-boost-libs-and-set-bamtools-paths (lambda* (#:key inputs #:allow-other-keys) (substitute* "CMakeLists.txt" (("set\\(Boost_USE_STATIC_LIBS ON\\)") "set(Boost_USE_STATIC_LIBS OFF)") (("\\$\\{CMAKE_CURRENT_SOURCE_DIR\\}/bamtools/include") (string-append (assoc-ref inputs "bamtools") "/include/bamtools"))) (substitute* "src/CMakeLists.txt" (("\\$\\{CMAKE_CURRENT_SOURCE_DIR\\}/\\.\\./bamtools/lib") (string-append (assoc-ref inputs "bamtools") "/lib/bamtools"))) #t) %standard-phases))) (inputs `(("boost" ,boost) ("bamtools" ,bamtools) ("protobuf" ,protobuf) ("zlib" ,zlib))) (home-page "http://bio.math.berkeley.edu/eXpress") (synopsis "Streaming quantification for high-throughput genomic sequencing") (description "eXpress is a streaming tool for quantifying the abundances of a set of target sequences from sampled subsequences. Example applications include transcript-level RNA-Seq quantification, allele-specific/haplotype expression analysis (from RNA-Seq), transcription factor binding quantification in ChIP-Seq, and analysis of metagenomic data.") (license license:artistic2.0))) (define-public express-beta-diversity (package (name "express-beta-diversity") (version "1.0.7") (source (origin (method url-fetch) (uri (string-append "https://github.com/dparks1134/ExpressBetaDiversity/archive/v" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "1djvdlmqvjf6h0zq7w36y8cl5cli6rgj86x65znl48agnwmzxfxr")))) (build-system gnu-build-system) (arguments `(#:phases (modify-phases %standard-phases (delete 'configure) (add-before 'build 'enter-source (lambda _ (chdir "source") #t)) (replace 'check (lambda _ (zero? (system* "../bin/ExpressBetaDiversity" "-u")))) (add-after 'check 'exit-source (lambda _ (chdir "..") #t)) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin"))) (mkdir-p bin) (copy-file "scripts/convertToEBD.py" (string-append bin "/convertToEBD.py")) (copy-file "bin/ExpressBetaDiversity" (string-append bin "/ExpressBetaDiversity")) #t)))))) (inputs `(("python" ,python-2))) (home-page "http://kiwi.cs.dal.ca/Software/ExpressBetaDiversity") (synopsis "Taxon- and phylogenetic-based beta diversity measures") (description "Express Beta Diversity (EBD) calculates ecological beta diversity (dissimilarity) measures between biological communities. EBD implements a variety of diversity measures including those that make use of phylogenetic similarity of community members.") (license license:gpl3+))) (define-public fasttree (package (name "fasttree") (version "2.1.9") (source (origin (method url-fetch) (uri (string-append "http://www.microbesonline.org/fasttree/FastTree-" version ".c")) (sha256 (base32 "0ljvvw8i1als1wbfzvrf15c3ii2vw9db20a259g6pzg34xyyb97k")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; no "check" target #:phases (modify-phases %standard-phases (delete 'unpack) (delete 'configure) (replace 'build (lambda* (#:key source #:allow-other-keys) (and (zero? (system* "gcc" "-O3" "-finline-functions" "-funroll-loops" "-Wall" "-o" "FastTree" source "-lm")) (zero? (system* "gcc" "-DOPENMP" "-fopenmp" "-O3" "-finline-functions" "-funroll-loops" "-Wall" "-o" "FastTreeMP" source "-lm"))))) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin"))) (mkdir-p bin) (copy-file "FastTree" (string-append bin "/FastTree")) (copy-file "FastTreeMP" (string-append bin "/FastTreeMP")) #t)))))) (home-page "http://www.microbesonline.org/fasttree") (synopsis "Infers approximately-maximum-likelihood phylogenetic trees") (description "FastTree can handle alignments with up to a million of sequences in a reasonable amount of time and memory. For large alignments, FastTree is 100-1,000 times faster than PhyML 3.0 or RAxML 7.") (license license:gpl2+))) (define-public fastx-toolkit (package (name "fastx-toolkit") (version "0.0.14") (source (origin (method url-fetch) (uri (string-append "https://github.com/agordon/fastx_toolkit/releases/download/" version "/fastx_toolkit-" version ".tar.bz2")) (sha256 (base32 "01jqzw386873sr0pjp1wr4rn8fsga2vxs1qfmicvx1pjr72007wy")))) (build-system gnu-build-system) (inputs `(("libgtextutils" ,libgtextutils))) (native-inputs `(("pkg-config" ,pkg-config))) (home-page "http://hannonlab.cshl.edu/fastx_toolkit/") (synopsis "Tools for FASTA/FASTQ file preprocessing") (description "The FASTX-Toolkit is a collection of command line tools for Short-Reads FASTA/FASTQ files preprocessing. Next-Generation sequencing machines usually produce FASTA or FASTQ files, containing multiple short-reads sequences. The main processing of such FASTA/FASTQ files is mapping the sequences to reference genomes. However, it is sometimes more productive to preprocess the files before mapping the sequences to the genome---manipulating the sequences to produce better mapping results. The FASTX-Toolkit tools perform some of these preprocessing tasks.") (license license:agpl3+))) (define-public flexbar (package (name "flexbar") (version "2.5") (source (origin (method url-fetch) (uri (string-append "mirror://sourceforge/flexbar/" version "/flexbar_v" version "_src.tgz")) (sha256 (base32 "13jaykc3y1x8y5nn9j8ljnb79s5y51kyxz46hdmvvjj6qhyympmf")))) (build-system cmake-build-system) (arguments `(#:configure-flags (list (string-append "-DFLEXBAR_BINARY_DIR=" (assoc-ref %outputs "out") "/bin/")) #:phases (alist-replace 'check (lambda* (#:key outputs #:allow-other-keys) (setenv "PATH" (string-append (assoc-ref outputs "out") "/bin:" (getenv "PATH"))) (chdir "../flexbar_v2.5_src/test") (zero? (system* "bash" "flexbar_validate.sh"))) (alist-delete 'install %standard-phases)))) (inputs `(("tbb" ,tbb) ("zlib" ,zlib))) (native-inputs `(("pkg-config" ,pkg-config) ("seqan" ,seqan))) (home-page "http://flexbar.sourceforge.net") (synopsis "Barcode and adapter removal tool for sequencing platforms") (description "Flexbar preprocesses high-throughput nucleotide sequencing data efficiently. It demultiplexes barcoded runs and removes adapter sequences. Moreover, trimming and filtering features are provided. Flexbar increases read mapping rates and improves genome and transcriptome assemblies. It supports next-generation sequencing data in fasta/q and csfasta/q format from Illumina, Roche 454, and the SOLiD platform.") (license license:gpl3))) (define-public fraggenescan (package (name "fraggenescan") (version "1.20") (source (origin (method url-fetch) (uri (string-append "mirror://sourceforge/fraggenescan/" "FragGeneScan" version ".tar.gz")) (sha256 (base32 "1zzigqmvqvjyqv4945kv6nc5ah2xxm1nxgrlsnbzav3f5c0n0pyj")))) (build-system gnu-build-system) (arguments `(#:phases (modify-phases %standard-phases (delete 'configure) (add-before 'build 'patch-paths (lambda* (#:key outputs #:allow-other-keys) (let* ((out (string-append (assoc-ref outputs "out"))) (share (string-append out "/share/fraggenescan/"))) (substitute* "run_FragGeneScan.pl" (("system\\(\"rm") (string-append "system(\"" (which "rm"))) (("system\\(\"mv") (string-append "system(\"" (which "mv"))) ;; This script and other programs expect the training files ;; to be in the non-standard location bin/train/XXX. Change ;; this to be share/fraggenescan/train/XXX instead. (("^\\$train.file = \\$dir.*") (string-append "$train_file = \"" share "train/\".$FGS_train_file;"))) (substitute* "run_hmm.c" (("^ strcat\\(train_dir, \\\"train/\\\"\\);") (string-append " strcpy(train_dir, \"" share "/train/\");"))) (substitute* "post_process.pl" (("^my \\$dir = substr.*") (string-append "my $dir = \"" share "\";")))) #t)) (replace 'build (lambda _ (and (zero? (system* "make" "clean")) (zero? (system* "make" "fgs"))))) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (string-append (assoc-ref outputs "out"))) (bin (string-append out "/bin/")) (share (string-append out "/share/fraggenescan/train"))) (install-file "run_FragGeneScan.pl" bin) (install-file "FragGeneScan" bin) (install-file "FGS_gff.py" bin) (install-file "post_process.pl" bin) (copy-recursively "train" share)))) (delete 'check) (add-after 'install 'post-install-check ;; In lieu of 'make check', run one of the examples and check the ;; output files gets created. (lambda* (#:key outputs #:allow-other-keys) (let* ((out (string-append (assoc-ref outputs "out"))) (bin (string-append out "/bin/"))) (and (zero? (system* (string-append bin "run_FragGeneScan.pl") "-genome=./example/NC_000913.fna" "-out=./test2" "-complete=1" "-train=complete")) (file-exists? "test2.faa") (file-exists? "test2.ffn") (file-exists? "test2.gff") (file-exists? "test2.out")))))))) (inputs `(("perl" ,perl) ("python" ,python-2))) ;not compatible with python 3. (home-page "https://sourceforge.net/projects/fraggenescan/") (synopsis "Finds potentially fragmented genes in short reads") (description "FragGeneScan is a program for predicting bacterial and archaeal genes in short and error-prone DNA sequencing reads. It can also be applied to predict genes in incomplete assemblies or complete genomes.") ;; GPL3+ according to private correspondense with the authors. (license license:gpl3+))) (define-public fxtract (let ((util-commit "776ca85a18a47492af3794745efcb4a905113115")) (package (name "fxtract") (version "2.3") (source (origin (method url-fetch) (uri (string-append "https://github.com/ctSkennerton/fxtract/archive/" version ".tar.gz")) (file-name (string-append "ctstennerton-util-" (string-take util-commit 7) "-checkout")) (sha256 (base32 "0275cfdhis8517hm01is62062swmi06fxzifq7mr3knbbxjlaiwj")))) (build-system gnu-build-system) (arguments `(#:make-flags (list (string-append "PREFIX=" (assoc-ref %outputs "out")) "CC=gcc") #:test-target "fxtract_test" #:phases (modify-phases %standard-phases (delete 'configure) (add-before 'build 'copy-util (lambda* (#:key inputs #:allow-other-keys) (rmdir "util") (copy-recursively (assoc-ref inputs "ctskennerton-util") "util") #t)) ;; Do not use make install as this requires additional dependencies. (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out"/bin"))) (install-file "fxtract" bin) #t)))))) (inputs `(("pcre" ,pcre) ("zlib" ,zlib))) (native-inputs ;; ctskennerton-util is licensed under GPL2. `(("ctskennerton-util" ,(origin (method git-fetch) (uri (git-reference (url "https://github.com/ctSkennerton/util.git") (commit util-commit))) (file-name (string-append "ctstennerton-util-" util-commit "-checkout")) (sha256 (base32 "0cls1hd4vgj3f36fpzzg4xc77d6f3hpc60cbpfmn2gdr7ykzzad7")))))) (home-page "https://github.com/ctSkennerton/fxtract") (synopsis "Extract sequences from FASTA and FASTQ files") (description "Fxtract extracts sequences from a protein or nucleotide fastx (FASTA or FASTQ) file given a subsequence. It uses a simple substring search for basic tasks but can change to using POSIX regular expressions, PCRE, hash lookups or multi-pattern searching as required. By default fxtract looks in the sequence of each record but can also be told to look in the header, comment or quality sections.") ;; 'util' requires SSE instructions. (supported-systems '("x86_64-linux")) (license license:expat)))) (define-public grit (package (name "grit") (version "2.0.2") (source (origin (method url-fetch) (uri (string-append "https://github.com/nboley/grit/archive/" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "157in84dj70wimbind3x7sy1whs3h57qfgcnj2s6lrd38fbrb7mj")))) (build-system python-build-system) (arguments `(#:python ,python-2 #:phases (alist-cons-after 'unpack 'generate-from-cython-sources (lambda* (#:key inputs outputs #:allow-other-keys) ;; Delete these C files to force fresh generation from pyx sources. (delete-file "grit/sparsify_support_fns.c") (delete-file "grit/call_peaks_support_fns.c") (substitute* "setup.py" (("Cython.Setup") "Cython.Build") ;; Add numpy include path to fix compilation (("pyx\", \\]") (string-append "pyx\", ], include_dirs = ['" (assoc-ref inputs "python-numpy") "/lib/python2.7/site-packages/numpy/core/include/" "']"))) #t) %standard-phases))) (inputs `(("python-scipy" ,python2-scipy) ("python-numpy" ,python2-numpy) ("python-pysam" ,python2-pysam) ("python-networkx" ,python2-networkx))) (native-inputs `(("python-cython" ,python2-cython) ("python-setuptools" ,python2-setuptools))) (home-page "http://grit-bio.org") (synopsis "Tool for integrative analysis of RNA-seq type assays") (description "GRIT is designed to use RNA-seq, TES, and TSS data to build and quantify full length transcript models. When none of these data sources are available, GRIT can be run by providing a candidate set of TES or TSS sites. In addition, GRIT can merge in reference junctions and gene boundaries. GRIT can also be run in quantification mode, where it uses a provided GTF file and just estimates transcript expression.") (license license:gpl3+))) (define-public hisat (package (name "hisat") (version "0.1.4") (source (origin (method url-fetch) (uri (string-append "http://ccb.jhu.edu/software/hisat/downloads/hisat-" version "-beta-source.zip")) (sha256 (base32 "1k381ydranqxp09yf2y7w1d0chz5d59vb6jchi89hbb0prq19lk5")))) (build-system gnu-build-system) (arguments `(#:tests? #f ;no check target #:make-flags '("allall" ;; Disable unsupported `popcnt' instructions on ;; architectures other than x86_64 ,@(if (string-prefix? "x86_64" (or (%current-target-system) (%current-system))) '() '("POPCNT_CAPABILITY=0"))) #:phases (alist-cons-after 'unpack 'patch-sources (lambda _ ;; XXX Cannot use snippet because zip files are not supported (substitute* "Makefile" (("^CC = .*$") "CC = gcc") (("^CPP = .*$") "CPP = g++") ;; replace BUILD_HOST and BUILD_TIME for deterministic build (("-DBUILD_HOST=.*") "-DBUILD_HOST=\"\\\"guix\\\"\"") (("-DBUILD_TIME=.*") "-DBUILD_TIME=\"\\\"0\\\"\"")) (substitute* '("hisat-build" "hisat-inspect") (("/usr/bin/env") (which "env")))) (alist-replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bi/"))) (for-each (lambda (file) (install-file file bin)) (find-files "." "hisat(-(build|align|inspect)(-(s|l)(-debug)*)*)*$")))) (alist-delete 'configure %standard-phases))))) (native-inputs `(("unzip" ,unzip))) (inputs `(("perl" ,perl) ("python" ,python) ("zlib" ,zlib))) ;; Non-portable SSE instructions are used so building fails on platforms ;; other than x86_64. (supported-systems '("x86_64-linux")) (home-page "http://ccb.jhu.edu/software/hisat/index.shtml") (synopsis "Hierarchical indexing for spliced alignment of transcripts") (description "HISAT is a fast and sensitive spliced alignment program for mapping RNA-seq reads. In addition to one global FM index that represents a whole genome, HISAT uses a large set of small FM indexes that collectively cover the whole genome. These small indexes (called local indexes) combined with several alignment strategies enable effective alignment of RNA-seq reads, in particular, reads spanning multiple exons.") (license license:gpl3+))) (define-public hmmer (package (name "hmmer") (version "3.1b2") (source (origin (method url-fetch) (uri (string-append "http://eddylab.org/software/hmmer" (version-prefix version 1) "/" version "/hmmer-" version ".tar.gz")) (sha256 (base32 "0djmgc0pfli0jilfx8hql1axhwhqxqb8rxg2r5rg07aw73sfs5nx")))) (build-system gnu-build-system) (native-inputs `(("perl" ,perl))) (home-page "http://hmmer.org/") (synopsis "Biosequence analysis using profile hidden Markov models") (description "HMMER is used for searching sequence databases for homologs of protein sequences, and for making protein sequence alignments. It implements methods using probabilistic models called profile hidden Markov models (profile HMMs).") (license (list license:gpl3+ ;; The bundled library 'easel' is distributed ;; under The Janelia Farm Software License. (license:non-copyleft "file://easel/LICENSE" "See easel/LICENSE in the distribution."))))) (define-public htseq (package (name "htseq") (version "0.6.1") (source (origin (method url-fetch) (uri (string-append "https://pypi.python.org/packages/source/H/HTSeq/HTSeq-" version ".tar.gz")) (sha256 (base32 "1i85ppf2j2lj12m0x690qq5nn17xxk23pbbx2c83r8ayb5wngzwv")))) (build-system python-build-system) (arguments `(#:python ,python-2)) ; only Python 2 is supported ;; Numpy needs to be propagated when htseq is used as a Python library. (propagated-inputs `(("python-numpy" ,python2-numpy))) (inputs `(("python-pysam" ,python2-pysam))) (native-inputs `(("python-setuptools" ,python2-setuptools))) (home-page "http://www-huber.embl.de/users/anders/HTSeq/") (synopsis "Analysing high-throughput sequencing data with Python") (description "HTSeq is a Python package that provides infrastructure to process data from high-throughput sequencing assays.") (license license:gpl3+))) (define-public java-htsjdk (package (name "java-htsjdk") (version "1.129") (source (origin (method url-fetch) (uri (string-append "https://github.com/samtools/htsjdk/archive/" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "0asdk9b8jx2ij7yd6apg9qx03li8q7z3ml0qy2r2qczkra79y6fw")) (modules '((guix build utils))) ;; remove build dependency on git (snippet '(substitute* "build.xml" (("failifexecutionfails=\"true\"") "failifexecutionfails=\"false\""))))) (build-system ant-build-system) (arguments `(#:tests? #f ; test require Internet access #:make-flags (list (string-append "-Ddist=" (assoc-ref %outputs "out") "/share/java/htsjdk/")) #:build-target "all" #:phases (modify-phases %standard-phases ;; The build phase also installs the jars (delete 'install)))) (home-page "http://samtools.github.io/htsjdk/") (synopsis "Java API for high-throughput sequencing data (HTS) formats") (description "HTSJDK is an implementation of a unified Java library for accessing common file formats, such as SAM and VCF, used for high-throughput sequencing (HTS) data. There are also an number of useful utilities for manipulating HTS data.") (license license:expat))) (define-public htslib (package (name "htslib") (version "1.3.1") (source (origin (method url-fetch) (uri (string-append "https://github.com/samtools/htslib/releases/download/" version "/htslib-" version ".tar.bz2")) (sha256 (base32 "1rja282fwdc25ql6izkhdyh8ppw8x2fs0w0js78zgkmqjlikmma9")))) (build-system gnu-build-system) (arguments `(#:phases (modify-phases %standard-phases (add-after 'unpack 'patch-tests (lambda _ (substitute* "test/test.pl" (("/bin/bash") (which "bash"))) #t))))) (inputs `(("zlib" ,zlib))) (native-inputs `(("perl" ,perl))) (home-page "http://www.htslib.org") (synopsis "C library for reading/writing high-throughput sequencing data") (description "HTSlib is a C library for reading/writing high-throughput sequencing data. It also provides the bgzip, htsfile, and tabix utilities.") ;; Files under cram/ are released under the modified BSD license; ;; the rest is released under the Expat license (license (list license:expat license:bsd-3)))) (define-public idr (package (name "idr") (version "2.0.0") (source (origin (method url-fetch) (uri (string-append "https://github.com/nboley/idr/archive/" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "1k3x44biak00aiv3hpm1yd6nn4hhp7n0qnbs3zh2q9sw7qr1qj5r")))) (build-system python-build-system) (arguments `(#:phases (modify-phases %standard-phases (add-after 'install 'wrap-program (lambda* (#:key inputs outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (python-version (string-take (string-take-right (assoc-ref inputs "python") 5) 3)) (path (string-join (map (lambda (name) (string-append (assoc-ref inputs name) "/lib/python" python-version "/site-packages")) '("python-scipy" "python-numpy" "python-matplotlib")) ":"))) (wrap-program (string-append out "/bin/idr") `("PYTHONPATH" ":" prefix (,path)))) #t))))) (inputs `(("python-scipy" ,python-scipy) ("python-numpy" ,python-numpy) ("python-matplotlib" ,python-matplotlib))) (native-inputs `(("python-cython" ,python-cython) ("python-setuptools" ,python-setuptools))) (home-page "https://github.com/nboley/idr") (synopsis "Tool to measure the irreproducible discovery rate (IDR)") (description "The IDR (Irreproducible Discovery Rate) framework is a unified approach to measure the reproducibility of findings identified from replicate experiments and provide highly stable thresholds based on reproducibility.") (license license:gpl3+))) (define-public jellyfish (package (name "jellyfish") (version "2.2.4") (source (origin (method url-fetch) (uri (string-append "https://github.com/gmarcais/Jellyfish/" "releases/download/v" version "/jellyfish-" version ".tar.gz")) (sha256 (base32 "0a6xnynqy2ibfbfz86b9g2m2dgm7f1469pmymkpam333gi3p26nk")))) (build-system gnu-build-system) (outputs '("out" ;for library "ruby" ;for Ruby bindings "python")) ;for Python bindings (arguments `(#:configure-flags (list (string-append "--enable-ruby-binding=" (assoc-ref %outputs "ruby")) (string-append "--enable-python-binding=" (assoc-ref %outputs "python"))) #:phases (modify-phases %standard-phases (add-before 'check 'set-SHELL-variable (lambda _ ;; generator_manager.hpp either uses /bin/sh or $SHELL ;; to run tests. (setenv "SHELL" (which "bash")) #t))))) (native-inputs `(("bc" ,bc) ("time" ,time) ("ruby" ,ruby) ("python" ,python-2))) (synopsis "Tool for fast counting of k-mers in DNA") (description "Jellyfish is a tool for fast, memory-efficient counting of k-mers in DNA. A k-mer is a substring of length k, and counting the occurrences of all such substrings is a central step in many analyses of DNA sequence. Jellyfish is a command-line program that reads FASTA and multi-FASTA files containing DNA sequences. It outputs its k-mer counts in a binary format, which can be translated into a human-readable text format using the @code{jellyfish dump} command, or queried for specific k-mers with @code{jellyfish query}.") (home-page "http://www.genome.umd.edu/jellyfish.html") ;; From their website: JELLYFISH runs on 64-bit Intel-compatible processors (supported-systems '("x86_64-linux")) ;; The combined work is published under the GPLv3 or later. Individual ;; files such as lib/jsoncpp.cpp are released under the Expat license. (license (list license:gpl3+ license:expat)))) (define-public khmer (package (name "khmer") (version "2.0") (source (origin (method url-fetch) (uri (pypi-uri "khmer" version)) (sha256 (base32 "0wb05shqh77v00256qlm68vbbx3kl76fyzihszbz5nhanl4ni33a")) (patches (search-patches "khmer-use-libraries.patch")))) (build-system python-build-system) (arguments `(#:phases (modify-phases %standard-phases (add-after 'unpack 'set-paths (lambda* (#:key inputs outputs #:allow-other-keys) ;; Delete bundled libraries. (delete-file-recursively "third-party/zlib") (delete-file-recursively "third-party/bzip2") ;; Replace bundled seqan. (let* ((seqan-all "third-party/seqan") (seqan-include (string-append seqan-all "/core/include"))) (delete-file-recursively seqan-all) (copy-recursively (string-append (assoc-ref inputs "seqan") "/include/seqan") (string-append seqan-include "/seqan"))) ;; We do not replace the bundled MurmurHash as the canonical ;; repository for this code 'SMHasher' is unsuitable for ;; providing a library. See ;; https://lists.gnu.org/archive/html/guix-devel/2016-06/msg00977.html #t)) (add-after 'unpack 'set-cc (lambda _ (setenv "CC" "gcc") #t)) ;; It is simpler to test after installation. (delete 'check) (add-after 'install 'post-install-check (lambda* (#:key inputs outputs #:allow-other-keys) (let ((out (assoc-ref outputs "out"))) (setenv "PATH" (string-append (getenv "PATH") ":" (assoc-ref outputs "out") "/bin")) (setenv "PYTHONPATH" (string-append (getenv "PYTHONPATH") ":" out "/lib/python" (string-take (string-take-right (assoc-ref inputs "python") 5) 3) "/site-packages")) (with-directory-excursion "build" (zero? (system* "nosetests" "khmer" "--attr" "!known_failing"))))))))) (native-inputs `(("seqan" ,seqan) ("python-nose" ,python-nose))) (inputs `(("zlib" ,zlib) ("bzip2" ,bzip2) ("python-screed" ,python-screed) ("python-bz2file" ,python-bz2file))) (home-page "https://khmer.readthedocs.org/") (synopsis "K-mer counting, filtering and graph traversal library") (description "The khmer software is a set of command-line tools for working with DNA shotgun sequencing data from genomes, transcriptomes, metagenomes and single cells. Khmer can make de novo assemblies faster, and sometimes better. Khmer can also identify and fix problems with shotgun data.") ;; When building on i686, armhf and mips64el, we get the following error: ;; error: ['khmer', 'khmer.tests', 'oxli'] require 64-bit operating system (supported-systems '("x86_64-linux")) (license license:bsd-3))) (define-public macs (package (name "macs") (version "2.1.0.20151222") (source (origin (method url-fetch) (uri (pypi-uri "MACS2" version)) (sha256 (base32 "1r2hcz6irhcq7lwbafjks98jbn34hv05avgbdjnp6w6mlfjkf8x5")))) (build-system python-build-system) (arguments `(#:python ,python-2 ; only compatible with Python 2.7 #:tests? #f)) ; no test target (inputs `(("python-numpy" ,python2-numpy))) (native-inputs `(("python-setuptools" ,python2-setuptools))) (home-page "http://github.com/taoliu/MACS/") (synopsis "Model based analysis for ChIP-Seq data") (description "MACS is an implementation of a ChIP-Seq analysis algorithm for identifying transcript factor binding sites named Model-based Analysis of ChIP-Seq (MACS). MACS captures the influence of genome complexity to evaluate the significance of enriched ChIP regions and it improves the spatial resolution of binding sites through combining the information of both sequencing tag position and orientation.") (license license:bsd-3))) (define-public mafft (package (name "mafft") (version "7.305") (source (origin (method url-fetch) (uri (string-append "http://mafft.cbrc.jp/alignment/software/mafft-" version "-without-extensions-src.tgz")) (file-name (string-append name "-" version ".tgz")) (sha256 (base32 "0ziim7g58n3z8gppsa713f5fxprl60ldj3xck186z0n9dpp06i8r")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; no automated tests, though there are tests in the read me #:make-flags (let ((out (assoc-ref %outputs "out"))) (list (string-append "PREFIX=" out) (string-append "BINDIR=" (string-append out "/bin")))) #:phases (modify-phases %standard-phases (add-after 'unpack 'enter-dir (lambda _ (chdir "core") #t)) (add-after 'enter-dir 'patch-makefile (lambda _ ;; on advice from the MAFFT authors, there is no need to ;; distribute mafft-profile, mafft-distance, or ;; mafft-homologs.rb as they are too "specialised". (substitute* "Makefile" ;; remove mafft-homologs.rb from SCRIPTS (("^SCRIPTS = mafft mafft-homologs.rb") "SCRIPTS = mafft") ;; remove mafft-homologs from MANPAGES (("^MANPAGES = mafft.1 mafft-homologs.1") "MANPAGES = mafft.1") ;; remove mafft-distance from PROGS (("^PROGS = dvtditr dndfast7 dndblast sextet5 mafft-distance") "PROGS = dvtditr dndfast7 dndblast sextet5") ;; remove mafft-profile from PROGS (("splittbfast disttbfast tbfast mafft-profile 2cl mccaskillwrap") "splittbfast disttbfast tbfast f2cl mccaskillwrap") (("^rm -f mafft-profile mafft-profile.exe") "#") (("^rm -f mafft-distance mafft-distance.exe") ")#") ;; do not install MAN pages in libexec folder (("^\t\\$\\(INSTALL\\) -m 644 \\$\\(MANPAGES\\) \ \\$\\(DESTDIR\\)\\$\\(LIBDIR\\)") "#")) #t)) (add-after 'enter-dir 'patch-paths (lambda* (#:key inputs #:allow-other-keys) (substitute* '("pairash.c" "mafft.tmpl") (("perl") (which "perl")) (("([\"`| ])awk" _ prefix) (string-append prefix (which "awk"))) (("grep") (which "grep"))) #t)) (delete 'configure) (add-after 'install 'wrap-programs (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin")) (path (string-append (assoc-ref %build-inputs "coreutils") "/bin:"))) (for-each (lambda (file) (wrap-program file `("PATH" ":" prefix (,path)))) (find-files bin))) #t))))) (inputs `(("perl" ,perl) ("ruby" ,ruby) ("gawk" ,gawk) ("grep" ,grep) ("coreutils" ,coreutils))) (home-page "http://mafft.cbrc.jp/alignment/software/") (synopsis "Multiple sequence alignment program") (description "MAFFT offers a range of multiple alignment methods for nucleotide and protein sequences. For instance, it offers L-INS-i (accurate; for alignment of <~200 sequences) and FFT-NS-2 (fast; for alignment of <~30,000 sequences).") (license (license:non-copyleft "http://mafft.cbrc.jp/alignment/software/license.txt" "BSD-3 with different formatting")))) (define-public mash (package (name "mash") (version "1.1.1") (source (origin (method url-fetch) (uri (string-append "https://github.com/marbl/mash/archive/v" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "08znbvqq5xknfhmpp3wcj574zvi4p7i8zifi67c9qw9a6ikp42fj")) (modules '((guix build utils))) (snippet ;; Delete bundled kseq. ;; TODO: Also delete bundled murmurhash and open bloom filter. '(delete-file "src/mash/kseq.h")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; No tests. #:configure-flags (list (string-append "--with-capnp=" (assoc-ref %build-inputs "capnproto")) (string-append "--with-gsl=" (assoc-ref %build-inputs "gsl"))) #:make-flags (list "CC=gcc") #:phases (modify-phases %standard-phases (add-after 'unpack 'fix-includes (lambda _ (substitute* '("src/mash/Sketch.cpp" "src/mash/CommandFind.cpp") (("^#include \"kseq\\.h\"") "#include \"htslib/kseq.h\"")) #t)) (add-before 'configure 'autoconf (lambda _ (zero? (system* "autoconf"))))))) (native-inputs `(("autoconf" ,autoconf) ;; Capnproto and htslib are statically embedded in the final ;; application. Therefore we also list their licenses, below. ("capnproto" ,capnproto) ("htslib" ,htslib))) (inputs `(("gsl" ,gsl) ("zlib" ,zlib))) (supported-systems '("x86_64-linux")) (home-page "https://mash.readthedocs.io") (synopsis "Fast genome and metagenome distance estimation using MinHash") (description "Mash is a fast sequence distance estimator that uses the MinHash algorithm and is designed to work with genomes and metagenomes in the form of assemblies or reads.") (license (list license:bsd-3 ; Mash license:expat ; HTSlib and capnproto license:public-domain ; MurmurHash 3 license:cpl1.0)))) ; Open Bloom Filter (define-public metabat ;; We package from a git commit because compilation of the released version ;; fails. (let ((commit "cbdca756993e66ae57e50a27970595dda9cbde1b")) (package (name "metabat") (version (string-append "0.32.4-1." (string-take commit 8))) (source (origin (method git-fetch) (uri (git-reference (url "https://bitbucket.org/berkeleylab/metabat.git") (commit commit))) (file-name (string-append name "-" version)) (sha256 (base32 "0byia8nsip6zvc4ha0qkxkxxyjf4x7jcvy48q2dvb0pzr989syzr")) (patches (search-patches "metabat-remove-compilation-date.patch")))) (build-system gnu-build-system) (arguments `(#:phases (modify-phases %standard-phases (add-after 'unpack 'fix-includes (lambda _ (substitute* "src/BamUtils.h" (("^#include \"bam/bam\\.h\"") "#include \"samtools/bam.h\"") (("^#include \"bam/sam\\.h\"") "#include \"samtools/sam.h\"")) (substitute* "src/KseqReader.h" (("^#include \"bam/kseq\\.h\"") "#include \"htslib/kseq.h\"")) #t)) (add-after 'unpack 'fix-scons (lambda* (#:key inputs #:allow-other-keys) (substitute* "SConstruct" (("^htslib_dir = 'samtools'") (string-append "hitslib_dir = '" (assoc-ref inputs "htslib") "'")) (("^samtools_dir = 'samtools'") (string-append "samtools_dir = '" (assoc-ref inputs "htslib") "'")) (("^findStaticOrShared\\('bam', hts_lib") (string-append "findStaticOrShared('bam', '" (assoc-ref inputs "samtools") "/lib'")) ;; Do not distribute README. (("^env\\.Install\\(idir_prefix, 'README\\.md'\\)") "")) #t)) (delete 'configure) (replace 'build (lambda* (#:key inputs outputs #:allow-other-keys) (mkdir (assoc-ref outputs "out")) (zero? (system* "scons" (string-append "PREFIX=" (assoc-ref outputs "out")) (string-append "BOOST_ROOT=" (assoc-ref inputs "boost")) "install")))) ;; Check and install are carried out during build phase. (delete 'check) (delete 'install)))) (inputs `(("zlib" ,zlib) ("perl" ,perl) ("samtools" ,samtools) ("htslib" ,htslib) ("boost" ,boost))) (native-inputs `(("scons" ,scons))) (home-page "https://bitbucket.org/berkeleylab/metabat") (synopsis "Reconstruction of single genomes from complex microbial communities") (description "Grouping large genomic fragments assembled from shotgun metagenomic sequences to deconvolute complex microbial communities, or metagenome binning, enables the study of individual organisms and their interactions. MetaBAT is an automated metagenome binning software, which integrates empirical probabilistic distances of genome abundance and tetranucleotide frequency.") (license (license:non-copyleft "file://license.txt" "See license.txt in the distribution."))))) (define-public minced (package (name "minced") (version "0.2.0") (source (origin (method url-fetch) (uri (string-append "https://github.com/ctSkennerton/minced/archive/" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "0wxmlsapxfpxfd3ps9636h7i2xy6la8i42mwh0j2lsky63h63jp1")))) (build-system gnu-build-system) (arguments `(#:test-target "test" #:phases (modify-phases %standard-phases (delete 'configure) (add-before 'check 'fix-test (lambda _ ;; Fix test for latest version. (substitute* "t/Aquifex_aeolicus_VF5.expected" (("minced:0.1.6") "minced:0.2.0")) #t)) (replace 'install ; No install target. (lambda* (#:key inputs outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin")) (wrapper (string-append bin "/minced"))) ;; Minced comes with a wrapper script that tries to figure out where ;; it is located before running the JAR. Since these paths are known ;; to us, we build our own wrapper to avoid coreutils dependency. (install-file "minced.jar" bin) (with-output-to-file wrapper (lambda _ (display (string-append "#!" (assoc-ref inputs "bash") "/bin/sh\n\n" (assoc-ref inputs "jre") "/bin/java -jar " bin "/minced.jar \"$@\"\n")))) (chmod wrapper #o555))))))) (native-inputs `(("jdk" ,icedtea "jdk"))) (inputs `(("bash" ,bash) ("jre" ,icedtea "out"))) (home-page "https://github.com/ctSkennerton/minced") (synopsis "Mining CRISPRs in Environmental Datasets") (description "MinCED is a program to find Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) in DNA sequences. It can be used for unassembled metagenomic reads, but is mainly designed for full genomes and assembled metagenomic sequence.") (license license:gpl3+))) (define-public miso (package (name "miso") (version "0.5.3") (source (origin (method url-fetch) (uri (string-append "https://pypi.python.org/packages/source/m/misopy/misopy-" version ".tar.gz")) (sha256 (base32 "0x446867az8ir0z8c1vjqffkp0ma37wm4sylixnkhgawllzx8v5w")) (modules '((guix build utils))) (snippet '(substitute* "setup.py" ;; Use setuptools, or else the executables are not ;; installed. (("distutils.core") "setuptools") ;; use "gcc" instead of "cc" for compilation (("^defines") "cc.set_executables( compiler='gcc', compiler_so='gcc', linker_exe='gcc', linker_so='gcc -shared'); defines"))))) (build-system python-build-system) (arguments `(#:python ,python-2 ; only Python 2 is supported #:tests? #f)) ; no "test" target (inputs `(("samtools" ,samtools) ("python-numpy" ,python2-numpy) ("python-pysam" ,python2-pysam) ("python-scipy" ,python2-scipy) ("python-matplotlib" ,python2-matplotlib))) (native-inputs `(("python-mock" ,python2-mock) ;for tests ("python-pytz" ,python2-pytz) ;for tests ("python-setuptools" ,python2-setuptools))) (home-page "http://genes.mit.edu/burgelab/miso/index.html") (synopsis "Mixture of Isoforms model for RNA-Seq isoform quantitation") (description "MISO (Mixture-of-Isoforms) is a probabilistic framework that quantitates the expression level of alternatively spliced genes from RNA-Seq data, and identifies differentially regulated isoforms or exons across samples. By modeling the generative process by which reads are produced from isoforms in RNA-Seq, the MISO model uses Bayesian inference to compute the probability that a read originated from a particular isoform.") (license license:gpl2))) (define-public muscle (package (name "muscle") (version "3.8.1551") (source (origin (method url-fetch/tarbomb) (file-name (string-append name "-" version)) (uri (string-append "http://www.drive5.com/muscle/muscle_src_" version ".tar.gz")) (sha256 (base32 "0bj8kj7sdizy3987zx6w7axihk40fk8rn76mpbqqjcnd64i5a367")))) (build-system gnu-build-system) (arguments `(#:make-flags (list "LDLIBS = -lm") #:phases (modify-phases %standard-phases (delete 'configure) (replace 'check ;; There are no tests, so just test if it runs. (lambda _ (zero? (system* "./muscle" "-version")))) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin"))) (install-file "muscle" bin))))))) (home-page "http://www.drive5.com/muscle") (synopsis "Multiple sequence alignment program") (description "MUSCLE aims to be a fast and accurate multiple sequence alignment program for nucleotide and protein sequences.") ;; License information found in 'muscle -h' and usage.cpp. (license license:public-domain))) (define-public orfm (package (name "orfm") (version "0.5.3") (source (origin (method url-fetch) (uri (string-append "https://github.com/wwood/OrfM/releases/download/v" version "/orfm-" version ".tar.gz")) (sha256 (base32 "0vb6d771gl4mix8bwx919x5ayy9pkj44n7ki336nz3rz2rx4c7gk")))) (build-system gnu-build-system) (inputs `(("zlib" ,zlib))) (native-inputs `(("ruby-bio-commandeer" ,ruby-bio-commandeer) ("ruby-rspec" ,ruby-rspec) ("ruby" ,ruby))) (synopsis "Simple and not slow open reading frame (ORF) caller") (description "An ORF caller finds stretches of DNA that, when translated, are not interrupted by stop codons. OrfM finds and prints these ORFs.") (home-page "https://github.com/wwood/OrfM") (license license:lgpl3+))) (define-public python2-pbcore (package (name "python2-pbcore") (version "1.2.10") (source (origin (method url-fetch) (uri (pypi-uri "pbcore" version)) (sha256 (base32 "1kjmv891d6qbpp4shhhvkl02ff4q5xlpnls2513sm2cjcrs52f1i")))) (build-system python-build-system) (arguments `(#:python ,python-2)) ; pbcore requires Python 2.7 (inputs `(("python-cython" ,python2-cython) ("python-numpy" ,python2-numpy) ("python-pysam" ,python2-pysam) ("python-h5py" ,python2-h5py))) (native-inputs `(("python-docutils" ,python2-docutils) ("python-nose" ,python2-nose) ("python-setuptools" ,python2-setuptools) ("python-sphinx" ,python2-sphinx))) (propagated-inputs `(("python-pyxb" ,python2-pyxb))) (home-page "http://pacificbiosciences.github.io/pbcore/") (synopsis "Library for reading and writing PacBio data files") (description "The pbcore package provides Python APIs for interacting with PacBio data files and writing bioinformatics applications.") (license license:bsd-3))) (define-public python2-warpedlmm (package (name "python2-warpedlmm") (version "0.21") (source (origin (method url-fetch) (uri (string-append "https://pypi.python.org/packages/source/W/WarpedLMM/WarpedLMM-" version ".zip")) (sha256 (base32 "1agfz6zqa8nc6cw47yh0s3y14gkpa9wqazwcj7mwwj3ffnw39p3j")))) (build-system python-build-system) (arguments `(#:python ,python-2 ; requires Python 2.7 #:phases (modify-phases %standard-phases (add-after 'install 'remove-bin-directory (lambda* (#:key outputs #:allow-other-keys) ;; The "bin" directory only contains wrappers for running ;; the module tests. They are not needed after the ;; "check" phase. (delete-file-recursively (string-append (assoc-ref outputs "out") "/bin")) #t))))) (propagated-inputs `(("python-scipy" ,python2-scipy) ("python-numpy" ,python2-numpy) ("python-matplotlib" ,python2-matplotlib) ("python-fastlmm" ,python2-fastlmm) ("python-pandas" ,python2-pandas) ("python-pysnptools" ,python2-pysnptools))) (native-inputs `(("python-setuptools" ,python2-setuptools) ("python-mock" ,python2-mock) ("python-nose" ,python2-nose) ("unzip" ,unzip))) (home-page "https://github.com/PMBio/warpedLMM") (synopsis "Implementation of warped linear mixed models") (description "WarpedLMM is a Python implementation of the warped linear mixed model, which automatically learns an optimal warping function (or transformation) for the phenotype as it models the data.") (license license:asl2.0))) (define-public pbtranscript-tofu (let ((commit "8f5467fe6a4472bcfb4226c8720993c8507adfe4")) (package (name "pbtranscript-tofu") (version (string-append "2.2.3." (string-take commit 7))) (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/PacificBiosciences/cDNA_primer.git") (commit commit))) (file-name (string-append name "-" version "-checkout")) (sha256 (base32 "1lgnpi35ihay42qx0b6yl3kkgra723i413j33kvs0kvs61h82w0f")) (modules '((guix build utils))) (snippet '(begin ;; remove bundled Cython sources (delete-file "pbtranscript-tofu/pbtranscript/Cython-0.20.1.tar.gz") #t)))) (build-system python-build-system) (arguments `(#:python ,python-2 ;; With standard flags, the install phase attempts to create a zip'd ;; egg file, and fails with an error: 'ZIP does not support timestamps ;; before 1980' #:configure-flags '("--single-version-externally-managed" "--record=pbtranscript-tofu.txt") #:phases (modify-phases %standard-phases (add-after 'unpack 'enter-directory (lambda _ (chdir "pbtranscript-tofu/pbtranscript/") #t)) ;; With setuptools version 18.0 and later this setup.py hack causes ;; a build error, so we disable it. (add-after 'enter-directory 'patch-setuppy (lambda _ (substitute* "setup.py" (("if 'setuptools.extension' in sys.modules:") "if False:")) #t))))) (inputs `(("python-numpy" ,python2-numpy) ("python-bx-python" ,python2-bx-python) ("python-networkx" ,python2-networkx) ("python-scipy" ,python2-scipy) ("python-pbcore" ,python2-pbcore) ("python-h5py" ,python2-h5py))) (native-inputs `(("python-cython" ,python2-cython) ("python-nose" ,python2-nose) ("python-setuptools" ,python2-setuptools))) (home-page "https://github.com/PacificBiosciences/cDNA_primer") (synopsis "Analyze transcriptome data generated with the Iso-Seq protocol") (description "pbtranscript-tofu contains scripts to analyze transcriptome data generated using the PacBio Iso-Seq protocol.") (license license:bsd-3)))) (define-public prank (package (name "prank") (version "150803") (source (origin (method url-fetch) (uri (string-append "http://wasabiapp.org/download/prank/prank.source." version ".tgz")) (sha256 (base32 "0am4z94fs3w2n5xpfls9zda61vq7qqz4q2i7b9hlsxz5q4j3kfm4")))) (build-system gnu-build-system) (arguments `(#:phases (modify-phases %standard-phases (add-after 'unpack 'enter-src-dir (lambda _ (chdir "src") #t)) (add-after 'unpack 'remove-m64-flag ;; Prank will build with the correct 'bit-ness' without this flag ;; and this allows building on 32-bit machines. (lambda _ (substitute* "src/Makefile" (("-m64") "")) #t)) (delete 'configure) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin")) (man (string-append out "/share/man/man1")) (path (string-append (assoc-ref %build-inputs "mafft") "/bin:" (assoc-ref %build-inputs "exonerate") "/bin:" (assoc-ref %build-inputs "bppsuite") "/bin"))) (install-file "prank" bin) (wrap-program (string-append bin "/prank") `("PATH" ":" prefix (,path))) (install-file "prank.1" man)) #t))))) (inputs `(("mafft" ,mafft) ("exonerate" ,exonerate) ("bppsuite" ,bppsuite))) (home-page "http://wasabiapp.org/software/prank/") (synopsis "Probabilistic multiple sequence alignment program") (description "PRANK is a probabilistic multiple sequence alignment program for DNA, codon and amino-acid sequences. It is based on a novel algorithm that treats insertions correctly and avoids over-estimation of the number of deletion events. In addition, PRANK borrows ideas from maximum likelihood methods used in phylogenetics and correctly takes into account the evolutionary distances between sequences. Lastly, PRANK allows for defining a potential structure for sequences to be aligned and then, simultaneously with the alignment, predicts the locations of structural units in the sequences.") (license license:gpl2+))) (define-public pyicoteo (package (name "pyicoteo") (version "2.0.7") (source (origin (method url-fetch) (uri (string-append "https://bitbucket.org/regulatorygenomicsupf/" "pyicoteo/get/v" version ".tar.bz2")) (file-name (string-append name "-" version ".tar.bz2")) (sha256 (base32 "0d6087f29xp8wxwlj111c3sylli98n0l8ry58c51ixzq0zfm50wa")))) (build-system python-build-system) (arguments `(#:python ,python-2 ; does not work with Python 3 #:tests? #f)) ; there are no tests (inputs `(("python2-matplotlib" ,python2-matplotlib))) (home-page "https://bitbucket.org/regulatorygenomicsupf/pyicoteo") (synopsis "Analyze high-throughput genetic sequencing data") (description "Pyicoteo is a suite of tools for the analysis of high-throughput genetic sequencing data. It works with genomic coordinates. There are currently six different command-line tools: @enumerate @item pyicoregion: for generating exploratory regions automatically; @item pyicoenrich: for differential enrichment between two conditions; @item pyicoclip: for calling CLIP-Seq peaks without a control; @item pyicos: for genomic coordinates manipulation; @item pyicoller: for peak calling on punctuated ChIP-Seq; @item pyicount: to count how many reads from N experiment files overlap in a region file; @item pyicotrocol: to combine operations from pyicoteo. @end enumerate\n") (license license:gpl3+))) (define-public prodigal (package (name "prodigal") (version "2.6.3") (source (origin (method url-fetch) (uri (string-append "https://github.com/hyattpd/Prodigal/archive/v" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "17srxkqd3jc77xk15pfbgg1a9xahqg7337w95mrsia7mpza4l2c9")))) (build-system gnu-build-system) (arguments `(#:tests? #f ;no check target #:make-flags (list (string-append "INSTALLDIR=" (assoc-ref %outputs "out") "/bin")) #:phases (modify-phases %standard-phases (delete 'configure)))) (home-page "http://prodigal.ornl.gov") (synopsis "Protein-coding gene prediction for Archaea and Bacteria") (description "Prodigal runs smoothly on finished genomes, draft genomes, and metagenomes, providing gene predictions in GFF3, Genbank, or Sequin table format. It runs quickly, in an unsupervised fashion, handles gaps, handles partial genes, and identifies translation initiation sites.") (license license:gpl3+))) (define-public roary (package (name "roary") (version "3.6.8") (source (origin (method url-fetch) (uri (string-append "mirror://cpan/authors/id/A/AJ/AJPAGE/Bio-Roary-" version ".tar.gz")) (sha256 (base32 "0g0pzcv8y7n2w8q7c9q0a7s2ghkwci6w8smg9mjw4agad5cd7yaw")))) (build-system perl-build-system) (arguments `(#:phases (modify-phases %standard-phases (delete 'configure) (delete 'build) (replace 'check (lambda _ ;; The tests are not run by default, so we run each test file ;; directly. (setenv "PATH" (string-append (getcwd) "/bin" ":" (getenv "PATH"))) (setenv "PERL5LIB" (string-append (getcwd) "/lib" ":" (getenv "PERL5LIB"))) (zero? (length (filter (lambda (file) (display file)(display "\n") (not (zero? (system* "perl" file)))) (find-files "t" ".*\\.t$")))))) (replace 'install ;; There is no 'install' target in the Makefile. (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin")) (perl (string-append out "/lib/perl5/site_perl")) (roary-plots "contrib/roary_plots")) (mkdir-p bin) (mkdir-p perl) (copy-recursively "bin" bin) (copy-recursively "lib" perl) #t))) (add-after 'install 'wrap-programs (lambda* (#:key inputs outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (perl5lib (getenv "PERL5LIB")) (path (getenv "PATH"))) (for-each (lambda (prog) (let ((binary (string-append out "/" prog))) (wrap-program binary `("PERL5LIB" ":" prefix (,(string-append perl5lib ":" out "/lib/perl5/site_perl")))) (wrap-program binary `("PATH" ":" prefix (,(string-append path ":" out "/bin")))))) (find-files "bin" ".*[^R]$")) (let ((file (string-append out "/bin/roary-create_pan_genome_plots.R")) (r-site-lib (getenv "R_LIBS_SITE")) (coreutils-path (string-append (assoc-ref inputs "coreutils") "/bin"))) (wrap-program file `("R_LIBS_SITE" ":" prefix (,(string-append r-site-lib ":" out "/site-library/")))) (wrap-program file `("PATH" ":" prefix (,(string-append coreutils-path ":" out "/bin")))))) #t))))) (native-inputs `(("perl-env-path" ,perl-env-path) ("perl-test-files" ,perl-test-files) ("perl-test-most" ,perl-test-most) ("perl-test-output" ,perl-test-output))) (inputs `(("perl-array-utils" ,perl-array-utils) ("bioperl" ,bioperl-minimal) ("perl-exception-class" ,perl-exception-class) ("perl-file-find-rule" ,perl-file-find-rule) ("perl-file-grep" ,perl-file-grep) ("perl-file-slurper" ,perl-file-slurper) ("perl-file-which" ,perl-file-which) ("perl-graph" ,perl-graph) ("perl-graph-readwrite" ,perl-graph-readwrite) ("perl-log-log4perl" ,perl-log-log4perl) ("perl-moose" ,perl-moose) ("perl-perlio-utf8_strict" ,perl-perlio-utf8_strict) ("perl-text-csv" ,perl-text-csv) ("bedtools" ,bedtools) ("cd-hit" ,cd-hit) ("blast+" ,blast+) ("mcl" ,mcl) ("parallel" ,parallel) ("prank" ,prank) ("mafft" ,mafft) ("fasttree" ,fasttree) ("grep" ,grep) ("sed" ,sed) ("gawk" ,gawk) ("r" ,r) ("r-ggplot2" ,r-ggplot2) ("coreutils" ,coreutils))) (home-page "http://sanger-pathogens.github.io/Roary") (synopsis "High speed stand-alone pan genome pipeline") (description "Roary is a high speed stand alone pan genome pipeline, which takes annotated assemblies in GFF3 format (produced by the Prokka program) and calculates the pan genome. Using a standard desktop PC, it can analyse datasets with thousands of samples, without compromising the quality of the results. 128 samples can be analysed in under 1 hour using 1 GB of RAM and a single processor. Roary is not intended for metagenomics or for comparing extremely diverse sets of genomes.") (license license:gpl3))) (define-public raxml (package (name "raxml") (version "8.2.9") (source (origin (method url-fetch) (uri (string-append "https://github.com/stamatak/standard-RAxML/archive/v" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "1pv8p2fy67y21a9y4cm7xpvxqjwz2v4201flfjshdq1p8j52rqf7")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; There are no tests. ;; Use 'standard' Makefile rather than SSE or AVX ones. #:make-flags (list "-f" "Makefile.HYBRID.gcc") #:phases (modify-phases %standard-phases (delete 'configure) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin")) (executable "raxmlHPC-HYBRID")) (install-file executable bin) (symlink (string-append bin "/" executable) "raxml")) #t))))) (inputs `(("openmpi" ,openmpi))) (home-page "http://sco.h-its.org/exelixis/web/software/raxml/index.html") (synopsis "Randomized Axelerated Maximum Likelihood phylogenetic trees") (description "RAxML is a tool for phylogenetic analysis and post-analysis of large phylogenies.") (license license:gpl2+))) (define-public rsem (package (name "rsem") (version "1.2.20") (source (origin (method url-fetch) (uri (string-append "http://deweylab.biostat.wisc.edu/rsem/src/rsem-" version ".tar.gz")) (sha256 (base32 "0nzdc0j0hjllhsd5f2xli95dafm3nawskigs140xzvjk67xh0r9q")) (patches (search-patches "rsem-makefile.patch")) (modules '((guix build utils))) (snippet '(begin ;; remove bundled copy of boost (delete-file-recursively "boost") #t)))) (build-system gnu-build-system) (arguments `(#:tests? #f ;no "check" target #:phases (modify-phases %standard-phases ;; No "configure" script. ;; Do not build bundled samtools library. (replace 'configure (lambda _ (substitute* "Makefile" (("^all : sam/libbam.a") "all : ")) #t)) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (string-append (assoc-ref outputs "out"))) (bin (string-append out "/bin/")) (perl (string-append out "/lib/perl5/site_perl"))) (mkdir-p bin) (mkdir-p perl) (for-each (lambda (file) (copy-file file (string-append bin (basename file)))) (find-files "." "rsem-.*")) (copy-file "rsem_perl_utils.pm" (string-append perl "/rsem_perl_utils.pm"))) #t)) (add-after 'install 'wrap-program (lambda* (#:key outputs #:allow-other-keys) (let ((out (assoc-ref outputs "out"))) (for-each (lambda (prog) (wrap-program (string-append out "/bin/" prog) `("PERL5LIB" ":" prefix (,(string-append out "/lib/perl5/site_perl"))))) '("rsem-plot-transcript-wiggles" "rsem-calculate-expression" "rsem-generate-ngvector" "rsem-run-ebseq" "rsem-prepare-reference"))) #t))))) (inputs `(("boost" ,boost) ("ncurses" ,ncurses) ("r" ,r) ("perl" ,perl) ("samtools" ,samtools-0.1) ("zlib" ,zlib))) (home-page "http://deweylab.biostat.wisc.edu/rsem/") (synopsis "Estimate gene expression levels from RNA-Seq data") (description "RSEM is a software package for estimating gene and isoform expression levels from RNA-Seq data. The RSEM package provides a user-friendly interface, supports threads for parallel computation of the EM algorithm, single-end and paired-end read data, quality scores, variable-length reads and RSPD estimation. In addition, it provides posterior mean and 95% credibility interval estimates for expression levels. For visualization, it can generate BAM and Wiggle files in both transcript-coordinate and genomic-coordinate.") (license license:gpl3+))) (define-public rseqc (package (name "rseqc") (version "2.6.1") (source (origin (method url-fetch) (uri (string-append "mirror://sourceforge/rseqc/" "RSeQC-" version ".tar.gz")) (sha256 (base32 "15ly0254yi032qzkdplg00q144qfdsd986gh62829rl5bkxhj330")) (modules '((guix build utils))) (snippet '(begin ;; remove bundled copy of pysam (delete-file-recursively "lib/pysam") (substitute* "setup.py" ;; remove dependency on outdated "distribute" module (("^from distribute_setup import use_setuptools") "") (("^use_setuptools\\(\\)") "") ;; do not use bundled copy of pysam (("^have_pysam = False") "have_pysam = True")))))) (build-system python-build-system) (arguments `(#:python ,python-2)) (inputs `(("python-cython" ,python2-cython) ("python-pysam" ,python2-pysam) ("python-numpy" ,python2-numpy) ("python-setuptools" ,python2-setuptools) ("zlib" ,zlib))) (native-inputs `(("python-nose" ,python2-nose))) (home-page "http://rseqc.sourceforge.net/") (synopsis "RNA-seq quality control package") (description "RSeQC provides a number of modules that can comprehensively evaluate high throughput sequence data, especially RNA-seq data. Some basic modules inspect sequence quality, nucleotide composition bias, PCR bias and GC bias, while RNA-seq specific modules evaluate sequencing saturation, mapped reads distribution, coverage uniformity, strand specificity, etc.") (license license:gpl3+))) (define-public seek ;; There are no release tarballs. According to the installation ;; instructions at http://seek.princeton.edu/installation.jsp, the latest ;; stable release is identified by this changeset ID. (let ((changeset "2329130") (revision "1")) (package (name "seek") (version (string-append "0-" revision "." changeset)) (source (origin (method hg-fetch) (uri (hg-reference (url "https://bitbucket.org/libsleipnir/sleipnir") (changeset changeset))) (sha256 (base32 "0qrvilwh18dpbhkf92qvxbmay0j75ra3jg2wrhz67gf538zzphsx")))) (build-system gnu-build-system) (arguments `(#:modules ((srfi srfi-1) (guix build gnu-build-system) (guix build utils)) #:phases (let ((dirs '("SeekMiner" "SeekEvaluator" "SeekPrep" "Distancer" "Data2DB" "PCL2Bin"))) (modify-phases %standard-phases (add-before 'configure 'bootstrap (lambda _ (zero? (system* "bash" "gen_auto")))) (add-after 'build 'build-additional-tools (lambda* (#:key make-flags #:allow-other-keys) (every (lambda (dir) (with-directory-excursion (string-append "tools/" dir) (zero? (apply system* "make" make-flags)))) dirs))) (add-after 'install 'install-additional-tools (lambda* (#:key make-flags #:allow-other-keys) (fold (lambda (dir result) (with-directory-excursion (string-append "tools/" dir) (and result (zero? (apply system* `("make" ,@make-flags "install")))))) #t dirs))))))) (inputs `(("gsl" ,gsl) ("boost" ,boost) ("libsvm" ,libsvm) ("readline" ,readline) ("gengetopt" ,gengetopt) ("log4cpp" ,log4cpp))) (native-inputs `(("autoconf" ,autoconf) ("automake" ,automake) ("perl" ,perl))) (home-page "http://seek.princeton.edu") (synopsis "Gene co-expression search engine") (description "SEEK is a computational gene co-expression search engine. SEEK provides biologists with a way to navigate the massive human expression compendium that now contains thousands of expression datasets. SEEK returns a robust ranking of co-expressed genes in the biological area of interest defined by the user's query genes. It also prioritizes thousands of expression datasets according to the user's query of interest.") (license license:cc-by3.0)))) (define-public samtools (package (name "samtools") (version "1.3.1") (source (origin (method url-fetch) (uri (string-append "mirror://sourceforge/samtools/samtools/" version "/samtools-" version ".tar.bz2")) (sha256 (base32 "0znnnxc467jbf1as2dpskrjhfh8mbll760j6w6rdkwlwbqsp8gbc")))) (build-system gnu-build-system) (arguments `(#:modules ((ice-9 ftw) (ice-9 regex) (guix build gnu-build-system) (guix build utils)) #:make-flags (list (string-append "prefix=" (assoc-ref %outputs "out"))) #:configure-flags (list "--with-ncurses") #:phases (alist-cons-after 'unpack 'patch-tests (lambda _ (substitute* "test/test.pl" ;; The test script calls out to /bin/bash (("/bin/bash") (which "bash"))) #t) (alist-cons-after 'install 'install-library (lambda* (#:key outputs #:allow-other-keys) (let ((lib (string-append (assoc-ref outputs "out") "/lib"))) (install-file "libbam.a" lib))) (alist-cons-after 'install 'install-headers (lambda* (#:key outputs #:allow-other-keys) (let ((include (string-append (assoc-ref outputs "out") "/include/samtools/"))) (for-each (lambda (file) (install-file file include)) (scandir "." (lambda (name) (string-match "\\.h$" name)))) #t)) %standard-phases))))) (native-inputs `(("pkg-config" ,pkg-config))) (inputs `(("ncurses" ,ncurses) ("perl" ,perl) ("python" ,python) ("zlib" ,zlib))) (home-page "http://samtools.sourceforge.net") (synopsis "Utilities to efficiently manipulate nucleotide sequence alignments") (description "Samtools implements various utilities for post-processing nucleotide sequence alignments in the SAM, BAM, and CRAM formats, including indexing, variant calling (in conjunction with bcftools), and a simple alignment viewer.") (license license:expat))) (define-public samtools-0.1 ;; This is the most recent version of the 0.1 line of samtools. The input ;; and output formats differ greatly from that used and produced by samtools ;; 1.x and is still used in many bioinformatics pipelines. (package (inherit samtools) (version "0.1.19") (source (origin (method url-fetch) (uri (string-append "mirror://sourceforge/samtools/samtools/" version "/samtools-" version ".tar.bz2")) (sha256 (base32 "1m33xsfwz0s8qi45lylagfllqg7fphf4dr0780rsvw75av9wk06h")))) (arguments `(#:tests? #f ;no "check" target ,@(substitute-keyword-arguments (package-arguments samtools) ((#:make-flags flags) `(cons "LIBCURSES=-lncurses" ,flags)) ((#:phases phases) `(modify-phases ,phases (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin"))) (mkdir-p bin) (copy-file "samtools" (string-append bin "/samtools"))))) (delete 'patch-tests) (delete 'configure)))))))) (define-public mosaik (let ((commit "5c25216d3522d6a33e53875cd76a6d65001e4e67")) (package (name "mosaik") (version "2.2.30") (source (origin ;; There are no release tarballs nor tags. (method git-fetch) (uri (git-reference (url "https://github.com/wanpinglee/MOSAIK.git") (commit commit))) (file-name (string-append name "-" version)) (sha256 (base32 "17gj3s07cm77r41z92awh0bim7w7q7fbn0sf5nkqmcm1vw052qgw")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; no tests #:make-flags (list "CC=gcc") #:phases (modify-phases %standard-phases (replace 'configure (lambda _ (chdir "src") #t)) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin"))) (mkdir-p bin) (copy-recursively "../bin" bin) #t)))))) (inputs `(("perl" ,perl) ("zlib" ,zlib))) (supported-systems '("x86_64-linux")) (home-page "https://code.google.com/p/mosaik-aligner/") (synopsis "Map nucleotide sequence reads to reference genomes") (description "MOSAIK is a program for mapping second and third-generation sequencing reads to a reference genome. MOSAIK can align reads generated by all the major sequencing technologies, including Illumina, Applied Biosystems SOLiD, Roche 454, Ion Torrent and Pacific BioSciences SMRT.") ;; MOSAIK is released under the GPLv2+ with the exception of third-party ;; code released into the public domain: ;; 1. fastlz by Ariya Hidayat - http://www.fastlz.org/ ;; 2. MD5 implementation - RSA Data Security, RFC 1321 (license (list license:gpl2+ license:public-domain))))) (define-public ngs-sdk (package (name "ngs-sdk") (version "1.2.5") (source (origin (method url-fetch) (uri (string-append "https://github.com/ncbi/ngs/archive/" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "04y1fsmdnb5y86m3gg6f5g9wcscr6r25n7m8mdlcxy0i2q6w6cia")))) (build-system gnu-build-system) (arguments `(#:parallel-build? #f ; not supported #:tests? #f ; no "check" target #:phases (alist-replace 'configure (lambda* (#:key outputs #:allow-other-keys) (let ((out (assoc-ref outputs "out"))) ;; The 'configure' script doesn't recognize things like ;; '--enable-fast-install'. (zero? (system* "./configure" (string-append "--build-prefix=" (getcwd) "/build") (string-append "--prefix=" out))))) (alist-cons-after 'unpack 'enter-dir (lambda _ (chdir "ngs-sdk") #t) %standard-phases)))) (native-inputs `(("perl" ,perl))) ;; According to the test ;; unless ($MARCH =~ /x86_64/i || $MARCH =~ /i?86/i) ;; in ngs-sdk/setup/konfigure.perl (supported-systems '("i686-linux" "x86_64-linux")) (home-page "https://github.com/ncbi/ngs") (synopsis "API for accessing Next Generation Sequencing data") (description "NGS is a domain-specific API for accessing reads, alignments and pileups produced from Next Generation Sequencing. The API itself is independent from any particular back-end implementation, and supports use of multiple back-ends simultaneously.") (license license:public-domain))) (define-public java-ngs (package (inherit ngs-sdk) (name "java-ngs") (arguments `(,@(substitute-keyword-arguments `(#:modules ((guix build gnu-build-system) (guix build utils) (srfi srfi-1) (srfi srfi-26)) ,@(package-arguments ngs-sdk)) ((#:phases phases) `(modify-phases ,phases (replace 'enter-dir (lambda _ (chdir "ngs-java") #t))))))) (inputs `(("jdk" ,icedtea "jdk") ("ngs-sdk" ,ngs-sdk))) (synopsis "Java bindings for NGS SDK"))) (define-public ncbi-vdb (package (name "ncbi-vdb") (version "2.7.0") (source (origin (method url-fetch) (uri (string-append "https://github.com/ncbi/ncbi-vdb/archive/" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "0x1cg1x8vy0yjlkp0snc1533zcjhxqzqsaiwqk598n7vvw37n8lf")))) (build-system gnu-build-system) (arguments `(#:parallel-build? #f ; not supported #:tests? #f ; no "check" target #:phases (alist-replace 'configure (lambda* (#:key inputs outputs #:allow-other-keys) (let ((out (assoc-ref outputs "out"))) ;; Override include path for libmagic (substitute* "setup/package.prl" (("name => 'magic', Include => '/usr/include'") (string-append "name=> 'magic', Include => '" (assoc-ref inputs "libmagic") "/include" "'"))) ;; Install kdf5 library (needed by sra-tools) (substitute* "build/Makefile.install" (("LIBRARIES_TO_INSTALL =") "LIBRARIES_TO_INSTALL = kdf5.$(VERSION_LIBX) kdf5.$(VERSION_SHLX)")) (substitute* "build/Makefile.env" (("CFLAGS =" prefix) (string-append prefix "-msse2 "))) ;; The 'configure' script doesn't recognize things like ;; '--enable-fast-install'. (zero? (system* "./configure" (string-append "--build-prefix=" (getcwd) "/build") (string-append "--prefix=" (assoc-ref outputs "out")) (string-append "--debug") (string-append "--with-xml2-prefix=" (assoc-ref inputs "libxml2")) (string-append "--with-ngs-sdk-prefix=" (assoc-ref inputs "ngs-sdk")) (string-append "--with-ngs-java-prefix=" (assoc-ref inputs "java-ngs")) (string-append "--with-hdf5-prefix=" (assoc-ref inputs "hdf5")))))) (alist-cons-after 'install 'install-interfaces (lambda* (#:key outputs #:allow-other-keys) ;; Install interface libraries. On i686 the interface libraries ;; are installed to "linux/gcc/i386", so we need to use the Linux ;; architecture name ("i386") instead of the target system prefix ;; ("i686"). (mkdir (string-append (assoc-ref outputs "out") "/ilib")) (copy-recursively (string-append "build/ncbi-vdb/linux/gcc/" ,(system->linux-architecture (or (%current-target-system) (%current-system))) "/rel/ilib") (string-append (assoc-ref outputs "out") "/ilib")) ;; Install interface headers (copy-recursively "interfaces" (string-append (assoc-ref outputs "out") "/include"))) %standard-phases)))) (inputs `(("libxml2" ,libxml2) ("ngs-sdk" ,ngs-sdk) ("java-ngs" ,java-ngs) ("libmagic" ,file) ("hdf5" ,hdf5))) (native-inputs `(("perl" ,perl))) ;; NCBI-VDB requires SSE capability. (supported-systems '("i686-linux" "x86_64-linux")) (home-page "https://github.com/ncbi/ncbi-vdb") (synopsis "Database engine for genetic information") (description "The NCBI-VDB library implements a highly compressed columnar data warehousing engine that is most often used to store genetic information. Databases are stored in a portable image within the file system, and can be accessed/downloaded on demand across HTTP.") (license license:public-domain))) (define-public plink (package (name "plink") (version "1.07") (source (origin (method url-fetch) (uri (string-append "http://pngu.mgh.harvard.edu/~purcell/plink/dist/plink-" version "-src.zip")) (sha256 (base32 "0as8gxm4pjyc8dxmm1sl873rrd7wn5qs0l29nqfnl31x8i467xaa")) (patches (search-patches "plink-1.07-unclobber-i.patch" "plink-endian-detection.patch")))) (build-system gnu-build-system) (arguments '(#:tests? #f ;no "check" target #:make-flags (list (string-append "LIB_LAPACK=" (assoc-ref %build-inputs "lapack") "/lib/liblapack.so") "WITH_LAPACK=1" "FORCE_DYNAMIC=1" ;; disable phoning home "WITH_WEBCHECK=") #:phases (modify-phases %standard-phases ;; no "configure" script (delete 'configure) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin/"))) (install-file "plink" bin) #t)))))) (inputs `(("zlib" ,zlib) ("lapack" ,lapack))) (native-inputs `(("unzip" ,unzip))) (home-page "http://pngu.mgh.harvard.edu/~purcell/plink/") (synopsis "Whole genome association analysis toolset") (description "PLINK is a whole genome association analysis toolset, designed to perform a range of basic, large-scale analyses in a computationally efficient manner. The focus of PLINK is purely on analysis of genotype/phenotype data, so there is no support for steps prior to this (e.g. study design and planning, generating genotype or CNV calls from raw data). Through integration with gPLINK and Haploview, there is some support for the subsequent visualization, annotation and storage of results.") ;; Code is released under GPLv2, except for fisher.h, which is under ;; LGPLv2.1+ (license (list license:gpl2 license:lgpl2.1+)))) (define-public smithlab-cpp (let ((revision "1") (commit "728a097bec88c6f4b8528b685932049e660eff2e")) (package (name "smithlab-cpp") (version (string-append "0." revision "." (string-take commit 7))) (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/smithlabcode/smithlab_cpp.git") (commit commit))) (file-name (string-append name "-" version "-checkout")) (sha256 (base32 "0d476lmj312xk77kr9fzrv7z1bv96yfyx0w7y62ycmnfbx32ll74")))) (build-system gnu-build-system) (arguments `(#:modules ((guix build gnu-build-system) (guix build utils) (srfi srfi-26)) #:tests? #f ;no "check" target #:phases (modify-phases %standard-phases (add-after 'unpack 'use-samtools-headers (lambda _ (substitute* '("SAM.cpp" "SAM.hpp") (("sam.h") "samtools/sam.h")) #t)) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (lib (string-append out "/lib")) (include (string-append out "/include/smithlab-cpp"))) (mkdir-p lib) (mkdir-p include) (for-each (cut install-file <> lib) (find-files "." "\\.o$")) (for-each (cut install-file <> include) (find-files "." "\\.hpp$"))) #t)) (delete 'configure)))) (inputs `(("samtools" ,samtools-0.1) ("zlib" ,zlib))) (home-page "https://github.com/smithlabcode/smithlab_cpp") (synopsis "C++ helper library for functions used in Smith lab projects") (description "Smithlab CPP is a C++ library that includes functions used in many of the Smith lab bioinformatics projects, such as a wrapper around Samtools data structures, classes for genomic regions, mapped sequencing reads, etc.") (license license:gpl3+)))) (define-public preseq (package (name "preseq") (version "2.0") (source (origin (method url-fetch) (uri (string-append "https://github.com/smithlabcode/" "preseq/archive/v" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "08r684l50pnxjpvmhzjgqq56yv9rfw90k8vx0nsrnrzk8mf9hsdq")) (modules '((guix build utils))) (snippet ;; Remove bundled samtools. '(delete-file-recursively "samtools")))) (build-system gnu-build-system) (arguments `(#:tests? #f ;no "check" target #:phases (modify-phases %standard-phases (delete 'configure)) #:make-flags (list (string-append "PREFIX=" (assoc-ref %outputs "out")) (string-append "LIBBAM=" (assoc-ref %build-inputs "samtools") "/lib/libbam.a") (string-append "SMITHLAB_CPP=" (assoc-ref %build-inputs "smithlab-cpp") "/lib") "PROGS=preseq" "INCLUDEDIRS=$(SMITHLAB_CPP)/../include/smithlab-cpp $(SAMTOOLS_DIR)"))) (inputs `(("gsl" ,gsl) ("samtools" ,samtools-0.1) ("smithlab-cpp" ,smithlab-cpp) ("zlib" ,zlib))) (home-page "http://smithlabresearch.org/software/preseq/") (synopsis "Program for analyzing library complexity") (description "The preseq package is aimed at predicting and estimating the complexity of a genomic sequencing library, equivalent to predicting and estimating the number of redundant reads from a given sequencing depth and how many will be expected from additional sequencing using an initial sequencing experiment. The estimates can then be used to examine the utility of further sequencing, optimize the sequencing depth, or to screen multiple libraries to avoid low complexity samples.") (license license:gpl3+))) (define-public python-screed (package (name "python-screed") (version "0.9") (source (origin (method url-fetch) (uri (pypi-uri "screed" version)) (sha256 (base32 "18czszp9fkx3j6jr7y5kp6dfialscgddk05mw1zkhh2zhn0jd8i0")))) (build-system python-build-system) (arguments `(#:phases (modify-phases %standard-phases (replace 'check (lambda _ (setenv "PYTHONPATH" (string-append (getenv "PYTHONPATH") ":.")) (zero? (system* "nosetests" "--attr" "!known_failing"))))))) (native-inputs `(("python-nose" ,python-nose))) (inputs `(("python-bz2file" ,python-bz2file))) (home-page "http://github.com/dib-lab/screed/") (synopsis "Short read sequence database utilities") (description "Screed parses FASTA and FASTQ files and generates databases. Values such as sequence name, sequence description, sequence quality and the sequence itself can be retrieved from these databases.") (license license:bsd-3))) (define-public python2-screed (let ((base (package-with-python2 (strip-python2-variant python-screed)))) (package (inherit base) (native-inputs `(("python2-setuptools" ,python2-setuptools) ,@(package-native-inputs base)))))) (define-public sra-tools (package (name "sra-tools") (version "2.7.0") (source (origin (method url-fetch) (uri (string-append "https://github.com/ncbi/sra-tools/archive/" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "13paw7bq6y47d2pl0ac5gpgcqp1xsy1g7v1fwysm3hr8lb2dck17")))) (build-system gnu-build-system) (arguments `(#:parallel-build? #f ; not supported #:tests? #f ; no "check" target #:make-flags (list (string-append "VDB_LIBDIR=" (assoc-ref %build-inputs "ncbi-vdb") ,(if (string-prefix? "x86_64" (or (%current-target-system) (%current-system))) "/lib64" "/lib32"))) #:phases (alist-replace 'configure (lambda* (#:key inputs outputs #:allow-other-keys) ;; The build system expects a directory containing the sources and ;; raw build output of ncbi-vdb, including files that are not ;; installed. Since we are building against an installed version of ;; ncbi-vdb, the following modifications are needed. (substitute* "setup/konfigure.perl" ;; Make the configure script look for the "ilib" directory of ;; "ncbi-vdb" without first checking for the existence of a ;; matching library in its "lib" directory. (("^ my \\$f = File::Spec->catdir\\(\\$libdir, \\$lib\\);") "my $f = File::Spec->catdir($ilibdir, $ilib);") ;; Look for interface libraries in ncbi-vdb's "ilib" directory. (("my \\$ilibdir = File::Spec->catdir\\(\\$builddir, 'ilib'\\);") "my $ilibdir = File::Spec->catdir($dir, 'ilib');")) ;; Dynamic linking (substitute* "tools/copycat/Makefile" (("smagic-static") "lmagic")) ;; The 'configure' script doesn't recognize things like ;; '--enable-fast-install'. (zero? (system* "./configure" (string-append "--build-prefix=" (getcwd) "/build") (string-append "--prefix=" (assoc-ref outputs "out")) (string-append "--debug") (string-append "--with-fuse-prefix=" (assoc-ref inputs "fuse")) (string-append "--with-magic-prefix=" (assoc-ref inputs "libmagic")) ;; TODO: building with libxml2 fails with linker errors ;; (string-append "--with-xml2-prefix=" ;; (assoc-ref inputs "libxml2")) (string-append "--with-ncbi-vdb-sources=" (assoc-ref inputs "ncbi-vdb")) (string-append "--with-ncbi-vdb-build=" (assoc-ref inputs "ncbi-vdb")) (string-append "--with-ngs-sdk-prefix=" (assoc-ref inputs "ngs-sdk")) (string-append "--with-hdf5-prefix=" (assoc-ref inputs "hdf5"))))) %standard-phases))) (native-inputs `(("perl" ,perl))) (inputs `(("ngs-sdk" ,ngs-sdk) ("ncbi-vdb" ,ncbi-vdb) ("libmagic" ,file) ("fuse" ,fuse) ("hdf5" ,hdf5) ("zlib" ,zlib))) (home-page "http://www.ncbi.nlm.nih.gov/Traces/sra/sra.cgi?view=software") (synopsis "Tools and libraries for reading and writing sequencing data") (description "The SRA Toolkit from NCBI is a collection of tools and libraries for reading of sequencing files from the Sequence Read Archive (SRA) database and writing files into the .sra format.") (license license:public-domain))) (define-public seqan (package (name "seqan") (version "1.4.2") (source (origin (method url-fetch) (uri (string-append "http://packages.seqan.de/seqan-library/" "seqan-library-" version ".tar.bz2")) (sha256 (base32 "05s3wrrwn50f81aklfm65i4a749zag1vr8z03k21xm0pdxy47yvp")))) ;; The documentation is 7.8MB and the includes are 3.6MB heavy, so it ;; makes sense to split the outputs. (outputs '("out" "doc")) (build-system trivial-build-system) (arguments `(#:modules ((guix build utils)) #:builder (begin (use-modules (guix build utils)) (let ((tar (assoc-ref %build-inputs "tar")) (bzip (assoc-ref %build-inputs "bzip2")) (out (assoc-ref %outputs "out")) (doc (assoc-ref %outputs "doc"))) (setenv "PATH" (string-append tar "/bin:" bzip "/bin")) (system* "tar" "xvf" (assoc-ref %build-inputs "source")) (chdir (string-append "seqan-library-" ,version)) (copy-recursively "include" (string-append out "/include")) (copy-recursively "share" (string-append doc "/share")))))) (native-inputs `(("source" ,source) ("tar" ,tar) ("bzip2" ,bzip2))) (home-page "http://www.seqan.de") (synopsis "Library for nucleotide sequence analysis") (description "SeqAn is a C++ library of efficient algorithms and data structures for the analysis of sequences with the focus on biological data. It contains algorithms and data structures for string representation and their manipulation, online and indexed string search, efficient I/O of bioinformatics file formats, sequence alignment, and more.") (license license:bsd-3))) (define-public seqmagick (package (name "seqmagick") (version "0.6.1") (source (origin (method url-fetch) (uri (string-append "https://pypi.python.org/packages/source/s/seqmagick/seqmagick-" version ".tar.gz")) (sha256 (base32 "0cgn477n74gsl4qdaakrrhi953kcsd4q3ivk2lr18x74s3g4ma1d")))) (build-system python-build-system) (arguments ;; python2 only, see https://github.com/fhcrc/seqmagick/issues/56 `(#:python ,python-2 #:phases (modify-phases %standard-phases ;; Current test in setup.py does not work as of 0.6.1, ;; so use nose to run tests instead for now. See ;; https://github.com/fhcrc/seqmagick/issues/55 (replace 'check (lambda _ (zero? (system* "nosetests"))))))) (inputs ;; biopython-1.66 is required due to ;; https://github.com/fhcrc/seqmagick/issues/59 ;; When that issue is resolved the 'python2-biopython-1.66' package ;; should be removed. `(("python-biopython" ,python2-biopython-1.66))) (native-inputs `(("python-setuptools" ,python2-setuptools) ("python-nose" ,python2-nose))) (home-page "http://github.com/fhcrc/seqmagick") (synopsis "Tools for converting and modifying sequence files") (description "Bioinformaticians often have to convert sequence files between formats and do little manipulations on them, and it's not worth writing scripts for that. Seqmagick is a utility to expose the file format conversion in BioPython in a convenient way. Instead of having a big mess of scripts, there is one that takes arguments.") (license license:gpl3))) (define-public seqtk (package (name "seqtk") (version "1.2") (source (origin (method url-fetch) (uri (string-append "https://github.com/lh3/seqtk/archive/v" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "0ywdyzpmfiz2wp6ampbzqg4y8bj450nfgqarpamg045b8mk32lxx")) (modules '((guix build utils))) (snippet '(begin ;; Remove extraneous header files, as is done in the seqtk ;; master branch. (for-each (lambda (file) (delete-file file)) (list "ksort.h" "kstring.h" "kvec.h")) #t)))) (build-system gnu-build-system) (arguments `(#:phases (modify-phases %standard-phases (delete 'configure) (replace 'check ;; There are no tests, so we just run a sanity check. (lambda _ (zero? (system* "./seqtk" "seq")))) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin/"))) (install-file "seqtk" bin))))))) (inputs `(("zlib" ,zlib))) (home-page "https://github.com/lh3/seqtk") (synopsis "Toolkit for processing biological sequences in FASTA/Q format") (description "Seqtk is a fast and lightweight tool for processing sequences in the FASTA or FASTQ format. It parses both FASTA and FASTQ files which can be optionally compressed by gzip.") (license license:expat))) (define-public snap-aligner (package (name "snap-aligner") (version "1.0beta.18") (source (origin (method url-fetch) (uri (string-append "https://github.com/amplab/snap/archive/v" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "1vnsjwv007k1fl1q7d681kbwn6bc66cgw6h16hym6gvyy71qv2ly")))) (build-system gnu-build-system) (arguments '(#:phases (modify-phases %standard-phases (delete 'configure) (replace 'check (lambda _ (zero? (system* "./unit_tests")))) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin"))) (mkdir-p bin) (install-file "snap-aligner" bin) (install-file "SNAPCommand" bin) #t)))))) (native-inputs `(("zlib" ,zlib))) (home-page "http://snap.cs.berkeley.edu/") (synopsis "Short read DNA sequence aligner") (description "SNAP is a fast and accurate aligner for short DNA reads. It is optimized for modern read lengths of 100 bases or higher, and takes advantage of these reads to align data quickly through a hash-based indexing scheme.") ;; 32-bit systems are not supported by the unpatched code. ;; Following the bug reports https://github.com/amplab/snap/issues/68 and ;; https://bugs.debian.org/cgi-bin/bugreport.cgi?bug=812378 we see that ;; systems without a lot of memory cannot make good use of this program. (supported-systems '("x86_64-linux")) (license license:asl2.0))) (define-public sortmerna (package (name "sortmerna") (version "2.1b") (source (origin (method url-fetch) (uri (string-append "https://github.com/biocore/sortmerna/archive/" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "1ghaghvd82af9j5adavxh77g7hm247d1r69m3fbi6f1jdivj5ldk")))) (build-system gnu-build-system) (outputs '("out" ;for binaries "db")) ;for sequence databases (arguments `(#:phases (modify-phases %standard-phases (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin")) (db (assoc-ref outputs "db")) (share (string-append db "/share/sortmerna/rRNA_databases"))) (install-file "sortmerna" bin) (install-file "indexdb_rna" bin) (for-each (lambda (file) (install-file file share)) (find-files "rRNA_databases" ".*fasta")) #t)))))) (inputs `(("zlib" ,zlib))) (home-page "http://bioinfo.lifl.fr/RNA/sortmerna") (synopsis "Biological sequence analysis tool for NGS reads") (description "SortMeRNA is a biological sequence analysis tool for filtering, mapping and operational taxonomic unit (OTU) picking of next generation sequencing (NGS) reads. The core algorithm is based on approximate seeds and allows for fast and sensitive analyses of nucleotide sequences. The main application of SortMeRNA is filtering rRNA from metatranscriptomic data.") (license license:lgpl3))) (define-public star (package (name "star") (version "2.5.2a") (source (origin (method url-fetch) (uri (string-append "https://github.com/alexdobin/STAR/archive/" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "0xjlsm4p9flln111hv4xx7xy94c2nl53zvdvbk9winmiradjsdra")) (modules '((guix build utils))) (snippet '(begin (substitute* "source/Makefile" (("/bin/rm") "rm")) ;; Remove pre-built binaries and bundled htslib sources. (delete-file-recursively "bin/MacOSX_x86_64") (delete-file-recursively "bin/Linux_x86_64") (delete-file-recursively "source/htslib") #t)))) (build-system gnu-build-system) (arguments '(#:tests? #f ;no check target #:make-flags '("STAR") #:phases (modify-phases %standard-phases (add-after 'unpack 'enter-source-dir (lambda _ (chdir "source") #t)) (add-after 'enter-source-dir 'do-not-use-bundled-htslib (lambda _ (substitute* "Makefile" (("(Depend.list: \\$\\(SOURCES\\) parametersDefault\\.xxd) htslib" _ prefix) prefix)) (substitute* '("BAMfunctions.cpp" "signalFromBAM.h" "bam_cat.h" "bam_cat.c" "STAR.cpp" "bamRemoveDuplicates.cpp") (("#include \"htslib/([^\"]+\\.h)\"" _ header) (string-append "#include <" header ">"))) (substitute* "IncludeDefine.h" (("\"htslib/(htslib/[^\"]+.h)\"" _ header) (string-append "<" header ">"))) #t)) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin/"))) (install-file "STAR" bin)) #t)) (delete 'configure)))) (native-inputs `(("vim" ,vim))) ; for xxd (inputs `(("htslib" ,htslib) ("zlib" ,zlib))) (home-page "https://github.com/alexdobin/STAR") (synopsis "Universal RNA-seq aligner") (description "The Spliced Transcripts Alignment to a Reference (STAR) software is based on a previously undescribed RNA-seq alignment algorithm that uses sequential maximum mappable seed search in uncompressed suffix arrays followed by seed clustering and stitching procedure. In addition to unbiased de novo detection of canonical junctions, STAR can discover non-canonical splices and chimeric (fusion) transcripts, and is also capable of mapping full-length RNA sequences.") ;; Only 64-bit systems are supported according to the README. (supported-systems '("x86_64-linux" "mips64el-linux")) ;; STAR is licensed under GPLv3 or later; htslib is MIT-licensed. (license license:gpl3+))) (define-public subread (package (name "subread") (version "1.5.1") (source (origin (method url-fetch) (uri (string-append "mirror://sourceforge/subread/subread-" version "/subread-" version "-source.tar.gz")) (sha256 (base32 "0gn5zhbvllks0mmdg3qlmsbg91p2mpdc2wixwfqpi85yzfrh8hcy")))) (build-system gnu-build-system) (arguments `(#:tests? #f ;no "check" target ;; The CC and CCFLAGS variables are set to contain a lot of x86_64 ;; optimizations by default, so we override these flags such that x86_64 ;; flags are only added when the build target is an x86_64 system. #:make-flags (list (let ((system ,(or (%current-target-system) (%current-system))) (flags '("-ggdb" "-fomit-frame-pointer" "-ffast-math" "-funroll-loops" "-fmessage-length=0" "-O9" "-Wall" "-DMAKE_FOR_EXON" "-DMAKE_STANDALONE" "-DSUBREAD_VERSION=\\\"${SUBREAD_VERSION}\\\"")) (flags64 '("-mmmx" "-msse" "-msse2" "-msse3"))) (if (string-prefix? "x86_64" system) (string-append "CCFLAGS=" (string-join (append flags flags64))) (string-append "CCFLAGS=" (string-join flags)))) "-f" "Makefile.Linux" "CC=gcc ${CCFLAGS}") #:phases (alist-cons-after 'unpack 'enter-dir (lambda _ (chdir "src") #t) (alist-replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin/"))) (mkdir-p bin) (copy-recursively "../bin" bin))) ;; no "configure" script (alist-delete 'configure %standard-phases))))) (inputs `(("zlib" ,zlib))) (home-page "http://bioinf.wehi.edu.au/subread-package/") (synopsis "Tool kit for processing next-gen sequencing data") (description "The subread package contains the following tools: subread aligner, a general-purpose read aligner; subjunc aligner: detecting exon-exon junctions and mapping RNA-seq reads; featureCounts: counting mapped reads for genomic features; exactSNP: a SNP caller that discovers SNPs by testing signals against local background noises.") (license license:gpl3+))) (define-public stringtie (package (name "stringtie") (version "1.2.1") (source (origin (method url-fetch) (uri (string-append "http://ccb.jhu.edu/software/stringtie/dl/" "stringtie-" version ".tar.gz")) (sha256 (base32 "1cqllsc1maq4kh92isi8yadgzbmnf042hlnalpk3y59aph1z3bfz")) (modules '((guix build utils))) (snippet '(begin (delete-file-recursively "samtools-0.1.18") #t)))) (build-system gnu-build-system) (arguments `(#:tests? #f ;no test suite #:phases (modify-phases %standard-phases ;; no configure script (delete 'configure) (add-before 'build 'use-system-samtools (lambda _ (substitute* "Makefile" (("stringtie: \\$\\{BAM\\}/libbam\\.a") "stringtie: ")) (substitute* '("gclib/GBam.h" "gclib/GBam.cpp") (("#include \"(bam|sam|kstring).h\"" _ header) (string-append "#include "))) #t)) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin/"))) (install-file "stringtie" bin) #t)))))) (inputs `(("samtools" ,samtools-0.1) ("zlib" ,zlib))) (home-page "http://ccb.jhu.edu/software/stringtie/") (synopsis "Transcript assembly and quantification for RNA-Seq data") (description "StringTie is a fast and efficient assembler of RNA-Seq sequence alignments into potential transcripts. It uses a novel network flow algorithm as well as an optional de novo assembly step to assemble and quantitate full-length transcripts representing multiple splice variants for each gene locus. Its input can include not only the alignments of raw reads used by other transcript assemblers, but also alignments of longer sequences that have been assembled from those reads. To identify differentially expressed genes between experiments, StringTie's output can be processed either by the Cuffdiff or Ballgown programs.") (license license:artistic2.0))) (define-public vcftools (package (name "vcftools") (version "0.1.14") (source (origin (method url-fetch) (uri (string-append "https://github.com/vcftools/vcftools/releases/download/v" version "/vcftools-" version ".tar.gz")) (sha256 (base32 "10l5c07z9p4i9pr4gl54b2c9h6ndhqlbq1rashg2zcgwkbfrkmvn")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; no "check" target #:make-flags (list "CFLAGS=-O2" ; override "-m64" flag (string-append "PREFIX=" (assoc-ref %outputs "out")) (string-append "MANDIR=" (assoc-ref %outputs "out") "/share/man/man1")))) (native-inputs `(("pkg-config" ,pkg-config))) (inputs `(("perl" ,perl) ("zlib" ,zlib))) (home-page "https://vcftools.github.io/") (synopsis "Tools for working with VCF files") (description "VCFtools is a program package designed for working with VCF files, such as those generated by the 1000 Genomes Project. The aim of VCFtools is to provide easily accessible methods for working with complex genetic variation data in the form of VCF files.") ;; The license is declared as LGPLv3 in the README and ;; at https://vcftools.github.io/license.html (license license:lgpl3))) (define-public infernal (package (name "infernal") (version "1.1.2") (source (origin (method url-fetch) (uri (string-append "http://eddylab.org/software/infernal/" "infernal-" version ".tar.gz")) (sha256 (base32 "0sr2hiz3qxfwqpz3whxr6n82p3x27336v3f34iqznp10hks2935c")))) (build-system gnu-build-system) (native-inputs `(("perl" ,perl))) ; for tests (home-page "http://eddylab.org/infernal/") (synopsis "Inference of RNA alignments") (description "Infernal (\"INFERence of RNA ALignment\") is a tool for searching DNA sequence databases for RNA structure and sequence similarities. It is an implementation of a special case of profile stochastic context-free grammars called @dfn{covariance models} (CMs). A CM is like a sequence profile, but it scores a combination of sequence consensus and RNA secondary structure consensus, so in many cases, it is more capable of identifying RNA homologs that conserve their secondary structure more than their primary sequence.") ;; Infernal 1.1.2 requires VMX or SSE capability for parallel instructions. (supported-systems '("i686-linux" "x86_64-linux")) (license license:bsd-3))) (define-public r-vegan (package (name "r-vegan") (version "2.4-1") (source (origin (method url-fetch) (uri (cran-uri "vegan" version)) (sha256 (base32 "0i0c7rc0nzgbysd1nlxzxd2rvy75qcnw3yc7nggzqjzzj5d7yzsd")))) (build-system r-build-system) (arguments `(#:phases (modify-phases %standard-phases (add-after 'unpack 'revert-test-deletion ;; The distributed sources do not include tests with the CRAN ;; package. Here we revert the commit ;; `591d0e8ba1deaaf82445474ec6619c0b43db4e63' which deletes these ;; tests. There are plans to not delete tests in future as ;; documented at https://github.com/vegandevs/vegan/issues/181. (lambda* (#:key inputs #:allow-other-keys) (zero? (system* "patch" "-R" "-p1" "-i" (assoc-ref inputs "r-vegan-delete-tests-patch")))))))) (native-inputs `(("gfortran" ,gfortran) ("r-knitr" ,r-knitr) ("r-vegan-delete-tests-patch" ,(origin (method url-fetch) (uri (string-append "https://github.com/vegandevs/vegan/commit/" "591d0e8ba1deaaf82445474ec6619c0b43db4e63.patch")) (sha256 (base32 "0b1bi7y4jjdl3ph721vm9apm51dr2z9piwvhy4355sf2b4kyyj5a")))))) (propagated-inputs `(("r-cluster" ,r-cluster) ("r-lattice" ,r-lattice) ("r-mgcv" ,r-mgcv) ("r-permute" ,r-permute))) (home-page "https://cran.r-project.org/web/packages/vegan") (synopsis "Functions for community ecology") (description "The vegan package provides tools for descriptive community ecology. It has most basic functions of diversity analysis, community ordination and dissimilarity analysis. Most of its multivariate tools can be used for other data types as well.") (license license:gpl2+))) (define-public r-annotate (package (name "r-annotate") (version "1.52.0") (source (origin (method url-fetch) (uri (bioconductor-uri "annotate" version)) (sha256 (base32 "1fd2csq7dcs2gwndgwdx2nwkymz8gsmlnqqzv3p0vjjsvvq5n2a8")))) (build-system r-build-system) (propagated-inputs `(("r-annotationdbi" ,r-annotationdbi) ("r-biobase" ,r-biobase) ("r-biocgenerics" ,r-biocgenerics) ("r-dbi" ,r-dbi) ("r-xml" ,r-xml) ("r-xtable" ,r-xtable))) (home-page "http://bioconductor.org/packages/annotate") (synopsis "Annotation for microarrays") (description "This package provides R enviroments for the annotation of microarrays.") (license license:artistic2.0))) (define-public r-geneplotter (package (name "r-geneplotter") (version "1.52.0") (source (origin (method url-fetch) (uri (bioconductor-uri "geneplotter" version)) (sha256 (base32 "1p6yvxi243irhjxwm97hp73abhwampj0myyf8z00ij166674pc7h")))) (build-system r-build-system) (propagated-inputs `(("r-annotate" ,r-annotate) ("r-annotationdbi" ,r-annotationdbi) ("r-biobase" ,r-biobase) ("r-biocgenerics" ,r-biocgenerics) ("r-lattice" ,r-lattice) ("r-rcolorbrewer" ,r-rcolorbrewer))) (home-page "http://bioconductor.org/packages/geneplotter") (synopsis "Graphics functions for genomic data") (description "This package provides functions for plotting genomic data.") (license license:artistic2.0))) (define-public r-genefilter (package (name "r-genefilter") (version "1.56.0") (source (origin (method url-fetch) (uri (bioconductor-uri "genefilter" version)) (sha256 (base32 "1vzgciqd09csqcw9qync8blsv51ylrd86a65iadgyy6j26g01fwd")))) (build-system r-build-system) (native-inputs `(("gfortran" ,gfortran))) (propagated-inputs `(("r-annotate" ,r-annotate) ("r-annotationdbi" ,r-annotationdbi) ("r-biobase" ,r-biobase) ("r-s4vectors" ,r-s4vectors))) (home-page "http://bioconductor.org/packages/genefilter") (synopsis "Filter genes from high-throughput experiments") (description "This package provides basic functions for filtering genes from high-throughput sequencing experiments.") (license license:artistic2.0))) (define-public r-deseq2 (package (name "r-deseq2") (version "1.14.0") (source (origin (method url-fetch) (uri (bioconductor-uri "DESeq2" version)) (sha256 (base32 "0kq06jy4xg5ii3a9l62f17kirsfx0gsiwq6mhiy985cqzpdn893g")))) (properties `((upstream-name . "DESeq2"))) (build-system r-build-system) (arguments `(#:phases (modify-phases %standard-phases (add-after 'unpack 'link-against-armadillo (lambda _ (substitute* "src/Makevars" (("PKG_LIBS =" prefix) (string-append prefix "-larmadillo")))))))) (propagated-inputs `(("r-biobase" ,r-biobase) ("r-biocgenerics" ,r-biocgenerics) ("r-biocparallel" ,r-biocparallel) ("r-genefilter" ,r-genefilter) ("r-geneplotter" ,r-geneplotter) ("r-genomicranges" ,r-genomicranges) ("r-ggplot2" ,r-ggplot2) ("r-hmisc" ,r-hmisc) ("r-iranges" ,r-iranges) ("r-locfit" ,r-locfit) ("r-rcpp" ,r-rcpp) ("r-rcpparmadillo" ,r-rcpparmadillo) ("r-s4vectors" ,r-s4vectors) ("r-summarizedexperiment" ,r-summarizedexperiment))) (home-page "http://bioconductor.org/packages/DESeq2") (synopsis "Differential gene expression analysis") (description "This package provides functions to estimate variance-mean dependence in count data from high-throughput nucleotide sequencing assays and test for differential expression based on a model using the negative binomial distribution.") (license license:lgpl3+))) (define-public r-annotationforge (package (name "r-annotationforge") (version "1.16.0") (source (origin (method url-fetch) (uri (bioconductor-uri "AnnotationForge" version)) (sha256 (base32 "02msyb9p3hywrryx00zpjkjl126mrv827i1ah1092s0cplm6xxvf")))) (properties `((upstream-name . "AnnotationForge"))) (build-system r-build-system) (propagated-inputs `(("r-annotationdbi" ,r-annotationdbi) ("r-biobase" ,r-biobase) ("r-biocgenerics" ,r-biocgenerics) ("r-dbi" ,r-dbi) ("r-rcurl" ,r-rcurl) ("r-rsqlite" ,r-rsqlite) ("r-s4vectors" ,r-s4vectors) ("r-xml" ,r-xml))) (home-page "http://bioconductor.org/packages/AnnotationForge") (synopsis "Code for building annotation database packages") (description "This package provides code for generating Annotation packages and their databases. Packages produced are intended to be used with AnnotationDbi.") (license license:artistic2.0))) (define-public r-rbgl (package (name "r-rbgl") (version "1.50.0") (source (origin (method url-fetch) (uri (bioconductor-uri "RBGL" version)) (sha256 (base32 "1q14m8w6ih56v680kf3d9wh1qbgp7af33kz3cxafdf1vvzx9km08")))) (properties `((upstream-name . "RBGL"))) (build-system r-build-system) (propagated-inputs `(("r-graph" ,r-graph))) (home-page "http://www.bioconductor.org/packages/RBGL") (synopsis "Interface to the Boost graph library") (description "This package provides a fairly extensive and comprehensive interface to the graph algorithms contained in the Boost library.") (license license:artistic2.0))) (define-public r-gseabase (package (name "r-gseabase") (version "1.36.0") (source (origin (method url-fetch) (uri (bioconductor-uri "GSEABase" version)) (sha256 (base32 "0l2x7yj7lfb0m2dmsav5ib026dikpgl4crdckrnj776yy08lgxpj")))) (properties `((upstream-name . "GSEABase"))) (build-system r-build-system) (propagated-inputs `(("r-annotate" ,r-annotate) ("r-annotationdbi" ,r-annotationdbi) ("r-biobase" ,r-biobase) ("r-biocgenerics" ,r-biocgenerics) ("r-graph" ,r-graph) ("r-xml" ,r-xml))) (home-page "http://bioconductor.org/packages/GSEABase") (synopsis "Gene set enrichment data structures and methods") (description "This package provides classes and methods to support @dfn{Gene Set Enrichment Analysis} (GSEA).") (license license:artistic2.0))) (define-public r-category (package (name "r-category") (version "2.40.0") (source (origin (method url-fetch) (uri (bioconductor-uri "Category" version)) (sha256 (base32 "16ncwz7b4y48k0p3fvbrbmvf7nfz63li9ysgcl8kp9kl4hg7llng")))) (properties `((upstream-name . "Category"))) (build-system r-build-system) (propagated-inputs `(("r-annotate" ,r-annotate) ("r-annotationdbi" ,r-annotationdbi) ("r-biobase" ,r-biobase) ("r-biocgenerics" ,r-biocgenerics) ("r-genefilter" ,r-genefilter) ("r-graph" ,r-graph) ("r-gseabase" ,r-gseabase) ("r-matrix" ,r-matrix) ("r-rbgl" ,r-rbgl) ("r-rsqlite" ,r-rsqlite))) (home-page "http://bioconductor.org/packages/Category") (synopsis "Category analysis") (description "This package provides a collection of tools for performing category analysis.") (license license:artistic2.0))) (define-public r-gostats (package (name "r-gostats") (version "2.40.0") (source (origin (method url-fetch) (uri (bioconductor-uri "GOstats" version)) (sha256 (base32 "0g2czm94zhzx92z7y2r4mjfxhwml7bhab2db6820ks8nkw1zvr9n")))) (properties `((upstream-name . "GOstats"))) (build-system r-build-system) (propagated-inputs `(("r-annotate" ,r-annotate) ("r-annotationdbi" ,r-annotationdbi) ("r-annotationforge" ,r-annotationforge) ("r-biobase" ,r-biobase) ("r-category" ,r-category) ("r-go-db" ,r-go-db) ("r-graph" ,r-graph) ("r-rbgl" ,r-rbgl))) (home-page "http://bioconductor.org/packages/GOstats") (synopsis "Tools for manipulating GO and microarrays") (description "This package provides a set of tools for interacting with GO and microarray data. A variety of basic manipulation tools for graphs, hypothesis testing and other simple calculations.") (license license:artistic2.0))) (define-public r-shortread (package (name "r-shortread") (version "1.32.0") (source (origin (method url-fetch) (uri (bioconductor-uri "ShortRead" version)) (sha256 (base32 "0mjdlg92x5qw4x2djc4dv5lxwl7ai6ix56nnf86zr07jk8vc7yls")))) (properties `((upstream-name . "ShortRead"))) (build-system r-build-system) (inputs `(("zlib" ,zlib))) (propagated-inputs `(("r-biobase" ,r-biobase) ("r-biocgenerics" ,r-biocgenerics) ("r-biocparallel" ,r-biocparallel) ("r-biostrings" ,r-biostrings) ("r-genomeinfodb" ,r-genomeinfodb) ("r-genomicalignments" ,r-genomicalignments) ("r-genomicranges" ,r-genomicranges) ("r-hwriter" ,r-hwriter) ("r-iranges" ,r-iranges) ("r-lattice" ,r-lattice) ("r-latticeextra" ,r-latticeextra) ("r-rsamtools" ,r-rsamtools) ("r-s4vectors" ,r-s4vectors) ("r-xvector" ,r-xvector) ("r-zlibbioc" ,r-zlibbioc))) (home-page "http://bioconductor.org/packages/ShortRead") (synopsis "FASTQ input and manipulation tools") (description "This package implements sampling, iteration, and input of FASTQ files. It includes functions for filtering and trimming reads, and for generating a quality assessment report. Data are represented as @code{DNAStringSet}-derived objects, and easily manipulated for a diversity of purposes. The package also contains legacy support for early single-end, ungapped alignment formats.") (license license:artistic2.0))) (define-public r-systempiper (package (name "r-systempiper") (version "1.8.1") (source (origin (method url-fetch) (uri (bioconductor-uri "systemPipeR" version)) (sha256 (base32 "0hyi841w8fm2yzpm6lwqi3jz5kc8ny8dy5p29dxynzaw5bpjw56d")))) (properties `((upstream-name . "systemPipeR"))) (build-system r-build-system) (propagated-inputs `(("r-annotate" ,r-annotate) ("r-batchjobs" ,r-batchjobs) ("r-biocgenerics" ,r-biocgenerics) ("r-biostrings" ,r-biostrings) ("r-deseq2" ,r-deseq2) ("r-edger" ,r-edger) ("r-genomicfeatures" ,r-genomicfeatures) ("r-genomicranges" ,r-genomicranges) ("r-ggplot2" ,r-ggplot2) ("r-go-db" ,r-go-db) ("r-gostats" ,r-gostats) ("r-limma" ,r-limma) ("r-pheatmap" ,r-pheatmap) ("r-rjson" ,r-rjson) ("r-rsamtools" ,r-rsamtools) ("r-shortread" ,r-shortread) ("r-summarizedexperiment" ,r-summarizedexperiment) ("r-variantannotation" ,r-variantannotation))) (home-page "https://github.com/tgirke/systemPipeR") (synopsis "Next generation sequencing workflow and reporting environment") (description "This R package provides tools for building and running automated end-to-end analysis workflows for a wide range of @dfn{next generation sequence} (NGS) applications such as RNA-Seq, ChIP-Seq, VAR-Seq and Ribo-Seq. Important features include a uniform workflow interface across different NGS applications, automated report generation, and support for running both R and command-line software, such as NGS aligners or peak/variant callers, on local computers or compute clusters. Efficient handling of complex sample sets and experimental designs is facilitated by a consistently implemented sample annotation infrastructure.") (license license:artistic2.0))) (define-public r-grohmm (package (name "r-grohmm") (version "1.8.0") (source (origin (method url-fetch) (uri (bioconductor-uri "groHMM" version)) (sha256 (base32 "0d91nyhqbi5hv3mgmr2z0g29wg2md26g0hyv5mgapmz20cd9zi4y")))) (properties `((upstream-name . "groHMM"))) (build-system r-build-system) (propagated-inputs `(("r-genomeinfodb" ,r-genomeinfodb) ("r-genomicalignments" ,r-genomicalignments) ("r-genomicranges" ,r-genomicranges) ("r-iranges" ,r-iranges) ("r-rtracklayer" ,r-rtracklayer) ("r-s4vectors" ,r-s4vectors))) (home-page "https://github.com/Kraus-Lab/groHMM") (synopsis "GRO-seq analysis pipeline") (description "This package provides a pipeline for the analysis of GRO-seq data.") (license license:gpl3+))) (define-public r-txdb-hsapiens-ucsc-hg19-knowngene (package (name "r-txdb-hsapiens-ucsc-hg19-knowngene") (version "3.2.2") (source (origin (method url-fetch) ;; We cannot use bioconductor-uri here because this tarball is ;; located under "data/annotation/" instead of "bioc/". (uri (string-append "http://bioconductor.org/packages/" "release/data/annotation/src/contrib" "/TxDb.Hsapiens.UCSC.hg19.knownGene_" version ".tar.gz")) (sha256 (base32 "1sajhcqqwazgz2lqbik7rd935i7kpnh08zxbp2ra10j72yqy4g86")))) (properties `((upstream-name . "TxDb.Hsapiens.UCSC.hg19.knownGene"))) (build-system r-build-system) ;; As this package provides little more than a very large data file it ;; doesn't make sense to build substitutes. (arguments `(#:substitutable? #f)) (propagated-inputs `(("r-genomicfeatures" ,r-genomicfeatures))) (home-page "http://bioconductor.org/packages/TxDb.Hsapiens.UCSC.hg19.knownGene/") (synopsis "Annotation package for human genome in TxDb format") (description "This package provides an annotation database of Homo sapiens genome data. It is derived from the UCSC hg19 genome and based on the \"knownGene\" track. The database is exposed as a @code{TxDb} object.") (license license:artistic2.0))) (define-public vsearch (package (name "vsearch") (version "2.3.0") (source (origin (method url-fetch) (uri (string-append "https://github.com/torognes/vsearch/archive/v" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "1r8fk3whkil348y5hfsd4r56qjmchhq4nxm6s7ra5rlisw0mf9fy")) (modules '((guix build utils))) (snippet '(begin ;; Remove bundled cityhash and '-mtune=native'. (substitute* "src/Makefile.am" (("^AM_CXXFLAGS=-I\\$\\{srcdir\\}/cityhash \ -O3 -mtune=native -Wall -Wsign-compare") (string-append "AM_CXXFLAGS=-lcityhash" " -O3 -Wall -Wsign-compare")) (("^__top_builddir__bin_vsearch_SOURCES = city.h \\\\") "__top_builddir__bin_vsearch_SOURCES = \\") (("^city.h \\\\") "\\") (("^citycrc.h \\\\") "\\") (("^libcityhash_a.*") "") (("noinst_LIBRARIES = libcpu_sse2.a libcpu_ssse3.a \ libcityhash.a") "noinst_LIBRARIES = libcpu_sse2.a libcpu_ssse3.a") (("__top_builddir__bin_vsearch_LDADD = libcpu_ssse3.a \ libcpu_sse2.a libcityhash.a") "__top_builddir__bin_vsearch_LDADD = libcpu_ssse3.a \ libcpu_sse2.a -lcityhash")) (substitute* "src/vsearch.h" (("^\\#include \"city.h\"") "#include ") (("^\\#include \"citycrc.h\"") "#include ")) (delete-file "src/city.h") (delete-file "src/citycrc.h") (delete-file "src/city.cc") #t)))) (build-system gnu-build-system) (arguments `(#:phases (modify-phases %standard-phases (add-before 'configure 'autogen (lambda _ (zero? (system* "autoreconf" "-vif"))))))) (inputs `(("zlib" ,zlib) ("bzip2" ,bzip2) ("cityhash" ,cityhash))) (native-inputs `(("autoconf" ,autoconf) ("automake" ,automake))) (synopsis "Sequence search tools for metagenomics") (description "VSEARCH supports DNA sequence searching, clustering, chimera detection, dereplication, pairwise alignment, shuffling, subsampling, sorting and masking. The tool takes advantage of parallelism in the form of SIMD vectorization as well as multiple threads to perform accurate alignments at high speed. VSEARCH uses an optimal global aligner (full dynamic programming Needleman-Wunsch).") (home-page "https://github.com/torognes/vsearch") ;; vsearch uses non-portable SSE intrinsics so building fails on other ;; platforms. (supported-systems '("x86_64-linux")) ;; Dual licensed; also includes public domain source. (license (list license:gpl3 license:bsd-2)))) (define-public pardre (package (name "pardre") (version "1.1.5") (source (origin (method url-fetch) (uri (string-append "mirror://sourceforge/pardre/ParDRe-rel" version ".tar.gz")) (sha256 (base32 "0zkyjzv4s8q2h5npalhirbk17r5b1h0n2a42mh7njzlf047h9bhy")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; no tests included #:phases (modify-phases %standard-phases (delete 'configure) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin"))) (mkdir-p bin) (install-file "ParDRe" bin) #t)))))) (inputs `(("openmpi" ,openmpi) ("zlib" ,zlib))) (synopsis "Parallel tool to remove duplicate DNA reads") (description "ParDRe is a parallel tool to remove duplicate genetic sequence reads. Duplicate reads can be seen as identical or nearly identical sequences with some mismatches. This tool lets users avoid the analysis of unnecessary reads, reducing the time of subsequent procedures with the dataset (e.g. assemblies, mappings, etc.). The tool is implemented with MPI in order to exploit the parallel capabilities of multicore clusters. It is faster than multithreaded counterparts (end of 2015) for the same number of cores and, thanks to the message-passing technology, it can be executed on clusters.") (home-page "https://sourceforge.net/projects/pardre/") (license license:gpl3+))) (define-public ruby-bio-kseq (package (name "ruby-bio-kseq") (version "0.0.2") (source (origin (method url-fetch) (uri (rubygems-uri "bio-kseq" version)) (sha256 (base32 "1xyaha46khb5jc6wzkbf7040jagac49jbimn0vcrzid0j8jdikrz")))) (build-system ruby-build-system) (arguments `(#:test-target "spec")) (native-inputs `(("bundler" ,bundler) ("ruby-rspec" ,ruby-rspec) ("ruby-rake-compiler" ,ruby-rake-compiler))) (inputs `(("zlib" ,zlib))) (synopsis "Ruby bindings for the kseq.h FASTA/Q parser") (description "@code{Bio::Kseq} provides ruby bindings to the @code{kseq.h} FASTA and FASTQ parsing code. It provides a fast iterator over sequences and their quality scores.") (home-page "https://github.com/gusevfe/bio-kseq") (license license:expat))) (define-public bio-locus (package (name "bio-locus") (version "0.0.7") (source (origin (method url-fetch) (uri (rubygems-uri "bio-locus" version)) (sha256 (base32 "02vmrxyimkj9sahsp4zhfhnmbvz6dbbqz1y01vglf8cbwvkajfl0")))) (build-system ruby-build-system) (native-inputs `(("ruby-rspec" ,ruby-rspec))) (synopsis "Tool for fast querying of genome locations") (description "Bio-locus is a tabix-like tool for fast querying of genome locations. Many file formats in bioinformatics contain records that start with a chromosome name and a position for a SNP, or a start-end position for indels. Bio-locus allows users to store this chr+pos or chr+pos+alt information in a database.") (home-page "https://github.com/pjotrp/bio-locus") (license license:expat))) (define-public bio-blastxmlparser (package (name "bio-blastxmlparser") (version "2.0.4") (source (origin (method url-fetch) (uri (rubygems-uri "bio-blastxmlparser" version)) (sha256 (base32 "1wf4qygcmdjgcqm6flmvsagfr1gs9lf63mj32qv3z1f481zc5692")))) (build-system ruby-build-system) (propagated-inputs `(("ruby-bio-logger" ,ruby-bio-logger) ("ruby-nokogiri" ,ruby-nokogiri))) (inputs `(("ruby-rspec" ,ruby-rspec))) (synopsis "Fast big data BLAST XML parser and library") (description "Very fast parallel big-data BLAST XML file parser which can be used as command line utility. Use blastxmlparser to: Parse BLAST XML; filter output; generate FASTA, JSON, YAML, RDF, JSON-LD, HTML, CSV, tabular output etc.") (home-page "http://github.com/pjotrp/blastxmlparser") (license license:expat))) (define-public bioruby (package (name "bioruby") (version "1.5.0") (source (origin (method url-fetch) (uri (rubygems-uri "bio" version)) (sha256 (base32 "01k2fyjl5fpx4zn8g6gqiqvsg2j1fgixrs9p03vzxckynxdq3wmc")))) (build-system ruby-build-system) (propagated-inputs `(("ruby-libxml" ,ruby-libxml))) (native-inputs `(("which" ,which))) ; required for test phase (arguments `(#:phases (modify-phases %standard-phases (add-before 'build 'patch-test-command (lambda _ (substitute* '("test/functional/bio/test_command.rb") (("/bin/sh") (which "sh"))) (substitute* '("test/functional/bio/test_command.rb") (("/bin/ls") (which "ls"))) (substitute* '("test/functional/bio/test_command.rb") (("which") (which "which"))) (substitute* '("test/functional/bio/test_command.rb", "test/data/command/echoarg2.sh") (("/bin/echo") (which "echo"))) #t))))) (synopsis "Ruby library, shell and utilities for bioinformatics") (description "BioRuby comes with a comprehensive set of Ruby development tools and libraries for bioinformatics and molecular biology. BioRuby has components for sequence analysis, pathway analysis, protein modelling and phylogenetic analysis; it supports many widely used data formats and provides easy access to databases, external programs and public web services, including BLAST, KEGG, GenBank, MEDLINE and GO.") (home-page "http://bioruby.org/") ;; Code is released under Ruby license, except for setup ;; (LGPLv2.1+) and scripts in samples (which have GPL2 and GPL2+) (license (list license:ruby license:lgpl2.1+ license:gpl2+ )))) (define-public r-acsnminer (package (name "r-acsnminer") (version "0.16.8.25") (source (origin (method url-fetch) (uri (cran-uri "ACSNMineR" version)) (sha256 (base32 "0gh604s8qall6zfjlwcg2ilxjvz08dplf9k5g47idhv43scm748l")))) (properties `((upstream-name . 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(uri (string-append "http://www.bioconductor.org/packages/" "release/data/annotation/src/contrib/" "BSgenome.Mmusculus.UCSC.mm9_" version ".tar.gz")) (sha256 (base32 "1birqw30g2azimxpnjfzmkphan7x131yy8b9h85lfz5fjdg7841i")))) (properties `((upstream-name . "BSgenome.Mmusculus.UCSC.mm9"))) (build-system r-build-system) ;; As this package provides little more than a very large data file it ;; doesn't make sense to build substitutes. (arguments `(#:substitutable? #f)) (propagated-inputs `(("r-bsgenome" ,r-bsgenome))) (home-page "http://www.bioconductor.org/packages/BSgenome.Mmusculus.UCSC.mm9/") (synopsis "Full genome sequences for Mouse") (description "This package provides full genome sequences for Mus musculus (Mouse) as provided by UCSC (mm9, July 2007) and stored in Biostrings objects.") 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(arguments `(#:substitutable? #f)) (propagated-inputs `(("r-bsgenome" ,r-bsgenome))) (home-page "http://www.bioconductor.org/packages/BSgenome.Dmelanogaster.UCSC.dm3/") (synopsis "Full genome sequences for Fly") (description "This package provides full genome sequences for Drosophila melanogaster (Fly) as provided by UCSC (dm3, April 2006) and stored in Biostrings objects.") (license license:artistic2.0))) (define-public r-motifrg (package (name "r-motifrg") (version "1.18.0") (source (origin (method url-fetch) (uri (bioconductor-uri "motifRG" version)) (sha256 (base32 "1pa97aj6c5f3gx4bgriw110764dj3m9h104ddi8rv2bpy41yd98d")))) (properties `((upstream-name . 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(license license:artistic2.0))) (define-public r-qtl (package (name "r-qtl") (version "1.39-5") (source (origin (method url-fetch) (uri (string-append "mirror://cran/src/contrib/qtl_" version ".tar.gz")) (sha256 (base32 "1grwgvyv7x0dgay1858bg7qf4wk47gpnq7qkqpcda9cn0h970d6f")))) (build-system r-build-system) (home-page "http://rqtl.org/") (synopsis "R package for analyzing QTL experiments in genetics") (description "R/qtl is an extension library for the R statistics system. It is used to analyze experimental crosses for identifying genes contributing to variation in quantitative traits (so-called quantitative trait loci, QTLs). Using a hidden Markov model, R/qtl allows to estimate genetic maps, to identify genotyping errors, and to perform single-QTL and two-QTL, two-dimensional genome scans.") (license license:gpl3))) (define-public r-zlibbioc (package (name "r-zlibbioc") (version "1.20.0") (source (origin (method url-fetch) (uri (bioconductor-uri "zlibbioc" version)) (sha256 (base32 "0hbk90q5hl0fycfvy5nxxa4hxgglag9lzp7i0fg849bqygg5nbyq")))) (properties `((upstream-name . "zlibbioc"))) (build-system r-build-system) (home-page "https://bioconductor.org/packages/zlibbioc") (synopsis "Provider for zlib-1.2.5 to R packages") (description "This package uses the source code of zlib-1.2.5 to create libraries for systems that do not have these available via other means.") (license license:artistic2.0))) (define-public r-rhtslib (package (name "r-rhtslib") (version "1.6.0") (source (origin (method url-fetch) (uri (bioconductor-uri "Rhtslib" version)) (sha256 (base32 "1vk3ng61dhi3pbia1lp3gl3mlr3i1vb2lkq83qb53i9dzz128wh9")))) (properties `((upstream-name . "Rhtslib"))) (build-system r-build-system) (propagated-inputs `(("r-zlibbioc" ,r-zlibbioc))) (inputs `(("zlib" ,zlib))) (native-inputs `(("autoconf" ,autoconf))) (home-page "https://github.com/nhayden/Rhtslib") (synopsis "High-throughput sequencing library as an R package") (description "This package provides the HTSlib C library for high-throughput nucleotide sequence analysis. The package is primarily useful to developers of other R packages who wish to make use of HTSlib.") (license license:lgpl2.0+))) (define-public r-bamsignals (package (name "r-bamsignals") (version "1.6.0") (source (origin (method url-fetch) (uri (bioconductor-uri "bamsignals" version)) (sha256 (base32 "1k42gvk5mgq4la1fp0in3an2zfdz69h6522jsqhmk0f6i75kg4mb")))) (build-system r-build-system) (propagated-inputs `(("r-biocgenerics" ,r-biocgenerics) ("r-genomicranges" ,r-genomicranges) ("r-iranges" ,r-iranges) ("r-rcpp" ,r-rcpp) ("r-rhtslib" ,r-rhtslib) ("r-zlibbioc" ,r-zlibbioc))) (inputs `(("zlib" ,zlib))) (home-page "http://bioconductor.org/packages/bamsignals") (synopsis "Extract read count signals from bam files") (description "This package allows to efficiently obtain count vectors from indexed bam files. It counts the number of nucleotide sequence reads in given genomic ranges and it computes reads profiles and coverage profiles. It also handles paired-end data.") (license license:gpl2+))) (define-public r-rcas (package (name "r-rcas") (version "1.0.0") (source (origin (method url-fetch) (uri (string-append "https://github.com/BIMSBbioinfo/RCAS/archive/v" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "1h7di822ihgkhmmmlfbfz3c2dkjyjxl307i6mx8w0cwjqbna1kp6")))) (build-system r-build-system) (native-inputs `(("r-knitr" ,r-knitr) ("r-testthat" ,r-testthat) ;; During vignette building knitr checks that "pandoc-citeproc" ;; is in the PATH. ("ghc-pandoc-citeproc" ,ghc-pandoc-citeproc))) (propagated-inputs `(("r-data-table" ,r-data-table) ("r-biomart" ,r-biomart) ("r-org-hs-eg-db" ,r-org-hs-eg-db) ("r-org-ce-eg-db" ,r-org-ce-eg-db) ("r-org-dm-eg-db" ,r-org-dm-eg-db) ("r-org-mm-eg-db" ,r-org-mm-eg-db) ("r-bsgenome-hsapiens-ucsc-hg19" ,r-bsgenome-hsapiens-ucsc-hg19) ("r-bsgenome-mmusculus-ucsc-mm9" ,r-bsgenome-mmusculus-ucsc-mm9) ("r-bsgenome-celegans-ucsc-ce10" ,r-bsgenome-celegans-ucsc-ce10) ("r-bsgenome-dmelanogaster-ucsc-dm3" ,r-bsgenome-dmelanogaster-ucsc-dm3) ("r-topgo" ,r-topgo) ("r-dt" ,r-dt) ("r-plotly" ,r-plotly) ("r-motifrg" ,r-motifrg) ("r-genomation" ,r-genomation) ("r-genomicfeatures" ,r-genomicfeatures) ("r-rtracklayer" ,r-rtracklayer) ("r-rmarkdown" ,r-rmarkdown))) (synopsis "RNA-centric annotation system") (description "RCAS aims to be a standalone RNA-centric annotation system that provides intuitive reports and publication-ready graphics. This package provides the R library implementing most of the pipeline's features.") (home-page "https://github.com/BIMSBbioinfo/RCAS") (license license:expat))) (define-public rcas-web (package (name "rcas-web") (version "0.0.3") (source (origin (method url-fetch) (uri (string-append "https://github.com/BIMSBbioinfo/rcas-web/" "releases/download/v" version "/rcas-web-" version ".tar.gz")) (sha256 (base32 "0d3my0g8i7js59n184zzzjdki7hgmhpi4rhfvk7i6jsw01ba04qq")))) (build-system gnu-build-system) (arguments `(#:phases (modify-phases %standard-phases (add-after 'install 'wrap-executable (lambda* (#:key inputs outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (json (assoc-ref inputs "guile-json")) (redis (assoc-ref inputs "guile-redis")) (path (string-append json "/share/guile/site/2.2:" redis "/share/guile/site/2.2"))) (wrap-program (string-append out "/bin/rcas-web") `("GUILE_LOAD_PATH" ":" = (,path)) `("GUILE_LOAD_COMPILED_PATH" ":" = (,path)) `("R_LIBS_SITE" ":" = (,(getenv "R_LIBS_SITE"))))) #t))))) (inputs `(("r" ,r) ("r-rcas" ,r-rcas) ("guile-next" ,guile-next) ("guile-json" ,guile2.2-json) ("guile-redis" ,guile2.2-redis))) (native-inputs `(("pkg-config" ,pkg-config))) (home-page "https://github.com/BIMSBbioinfo/rcas-web") (synopsis "Web interface for RNA-centric annotation system (RCAS)") (description "This package provides a simple web interface for the @dfn{RNA-centric annotation system} (RCAS).") (license license:agpl3+))) (define-public r-mutationalpatterns (package (name "r-mutationalpatterns") (version "1.0.0") (source (origin (method url-fetch) (uri (bioconductor-uri "MutationalPatterns" version)) (sha256 (base32 "1a3c2bm0xx0q4gf98jiw74msmdf2fr8rbsdysd5ww9kqlzmsbr17")))) (build-system r-build-system) (propagated-inputs `(("r-biocgenerics" ,r-biocgenerics) ("r-biostrings" ,r-biostrings) ("r-genomicranges" ,r-genomicranges) ("r-genomeinfodb" ,r-genomeinfodb) ("r-ggplot2" ,r-ggplot2) ("r-gridextra" ,r-gridextra) ("r-iranges" ,r-iranges) ("r-nmf" ,r-nmf) ("r-plyr" ,r-plyr) ("r-pracma" ,r-pracma) ("r-reshape2" ,r-reshape2) ("r-summarizedexperiment" ,r-summarizedexperiment) ("r-variantannotation" ,r-variantannotation))) (home-page "http://bioconductor.org/packages/MutationalPatterns/") (synopsis "Extract and visualize mutational patterns in genomic data") (description "This package provides an extensive toolset for the characterization and visualization of a wide range of mutational patterns in SNV base substitution data.") (license license:expat))) (define-public emboss (package (name "emboss") (version "6.5.7") (source (origin (method url-fetch) (uri (string-append "ftp://emboss.open-bio.org/pub/EMBOSS/old/" (version-major+minor version) ".0/" "EMBOSS-" version ".tar.gz")) (sha256 (base32 "0vsmz96gc411yj2iyzdrsmg4l2n1nhgmp7vrgzlxx3xixv9xbf0q")))) (build-system gnu-build-system) (arguments `(#:configure-flags (list (string-append "--with-hpdf=" (assoc-ref %build-inputs "libharu"))) #:phases (modify-phases %standard-phases (add-after 'unpack 'fix-checks (lambda _ ;; The PNGDRIVER tests check for the presence of libgd, libpng ;; and zlib, but assume that they are all found at the same ;; prefix. (substitute* "configure.in" (("CHECK_PNGDRIVER") "LIBS=\"$LIBS -lgd -lpng -lz -lm\" AC_DEFINE([PLD_png], [1], [Define to 1 if PNG support is available]) AM_CONDITIONAL(AMPNG, true)")) #t)) (add-after 'unpack 'disable-update-check (lambda _ ;; At build time there is no connection to the Internet, so ;; looking for updates will not work. (substitute* "Makefile.am" (("\\$\\(bindir\\)/embossupdate") "")) #t)) (add-before 'configure 'autogen (lambda _ (zero? (system* "autoreconf" "-vif"))))))) (inputs `(("perl" ,perl) ("libpng" ,libpng) ("gd" ,gd) ("libx11" ,libx11) ("libharu" ,libharu) ("zlib" ,zlib))) (native-inputs `(("autoconf" ,autoconf) ("automake" ,automake) ("libtool" ,libtool) ("pkg-config" ,pkg-config))) (home-page "http://emboss.sourceforge.net") (synopsis "Molecular biology analysis suite") (description "EMBOSS is the \"European Molecular Biology Open Software Suite\". EMBOSS is an analysis package specially developed for the needs of the molecular biology (e.g. EMBnet) user community. The software automatically copes with data in a variety of formats and even allows transparent retrieval of sequence data from the web. It also provides a number of libraries for the development of software in the field of molecular biology. EMBOSS also integrates a range of currently available packages and tools for sequence analysis into a seamless whole.") (license license:gpl2+))) (define-public bits (let ((revision "1") (commit "3cc4567896d9d6442923da944beb704750a08d2d")) (package (name "bits") ;; The version is 2.13.0 even though no release archives have been ;; published as yet. (version (string-append "2.13.0-" revision "." (string-take commit 9))) (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/arq5x/bits.git") (commit commit))) (file-name (string-append name "-" version "-checkout")) (sha256 (base32 "17n2kffk4kmhivd8c98g2vr6y1s23vbg4sxlxs689wni66797hbs")))) (build-system gnu-build-system) (arguments `(#:tests? #f ;no tests included #:phases (modify-phases %standard-phases (delete 'configure) (add-after 'unpack 'remove-cuda (lambda _ (substitute* "Makefile" ((".*_cuda") "") (("(bits_test_intersections) \\\\" _ match) match)) #t)) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (copy-recursively "bin" (string-append (assoc-ref outputs "out") "/bin")) #t))))) (inputs `(("gsl" ,gsl) ("zlib" ,zlib))) (home-page "https://github.com/arq5x/bits") (synopsis "Implementation of binary interval search algorithm") (description "This package provides an implementation of the BITS (Binary Interval Search) algorithm, an approach to interval set intersection. It is especially suited for the comparison of diverse genomic datasets and the exploration of large datasets of genome intervals (e.g. genes, sequence alignments).") (license license:gpl2)))) (define-public piranha ;; There is no release tarball for the latest version. The latest commit is ;; older than one year at the time of this writing. (let ((revision "1") (commit "0466d364b71117d01e4471b74c514436cc281233")) (package (name "piranha") (version (string-append "1.2.1-" revision "." (string-take commit 9))) (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/smithlabcode/piranha.git") (commit commit))) (sha256 (base32 "117dc0zf20c61jam69sk4abl57ah6yi6i7qra7d7y5zrbgk12q5n")))) (build-system gnu-build-system) (arguments `(#:test-target "test" #:phases (modify-phases %standard-phases (add-after 'unpack 'copy-smithlab-cpp (lambda* (#:key inputs #:allow-other-keys) (for-each (lambda (file) (install-file file "./src/smithlab_cpp/")) (find-files (assoc-ref inputs "smithlab-cpp"))) #t)) (add-after 'install 'install-to-store (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin"))) (mkdir-p bin) (for-each (lambda (file) (install-file file bin)) (find-files "bin" ".*"))) #t))) #:configure-flags (list (string-append "--with-bam_tools_headers=" (assoc-ref %build-inputs "bamtools") "/include/bamtools") (string-append "--with-bam_tools_library=" (assoc-ref %build-inputs "bamtools") "/lib/bamtools")))) (inputs `(("bamtools" ,bamtools) ("samtools" ,samtools-0.1) ("gsl" ,gsl) ("smithlab-cpp" ,(let ((commit "3723e2db438c51501d0423429ff396c3035ba46a")) (origin (method git-fetch) (uri (git-reference (url "https://github.com/smithlabcode/smithlab_cpp.git") (commit commit))) (file-name (string-append "smithlab_cpp-" commit "-checkout")) (sha256 (base32 "0l4gvbwslw5ngziskja41c00x1r06l3yidv7y0xw9djibhykzy0g"))))))) (native-inputs `(("python" ,python-2))) (home-page "https://github.com/smithlabcode/piranha") (synopsis "Peak-caller for CLIP-seq and RIP-seq data") (description "Piranha is a peak-caller for genomic data produced by CLIP-seq and RIP-seq experiments. It takes input in BED or BAM format and identifies regions of statistically significant read enrichment. Additional covariates may optionally be provided to further inform the peak-calling process.") (license license:gpl3+)))) (define-public pepr (package (name "pepr") (version "1.0.9") (source (origin (method url-fetch) (uri (string-append "https://pypi.python.org/packages/source/P" "/PePr/PePr-" version ".tar.gz")) (sha256 (base32 "0qxjfdpl1b1y53nccws2d85f6k74zwmx8y8sd9rszcqhfayx6gdx")))) (build-system python-build-system) (arguments `(#:python ,python-2 ; python2 only #:tests? #f ; no tests included #:phases (modify-phases %standard-phases ;; When setuptools is used a ".egg" archive is generated and ;; installed. This makes it hard to actually run PePr. This issue ;; has been reported upstream: ;; https://github.com/shawnzhangyx/PePr/issues/9 (add-after 'unpack 'disable-egg-generation (lambda _ (substitute* "setup.py" (("from setuptools import setup") "from distutils.core import setup")) #t))))) (propagated-inputs `(("python2-numpy" ,python2-numpy) ("python2-scipy" ,python2-scipy) ("python2-pysam" ,python2-pysam))) (home-page "https://code.google.com/p/pepr-chip-seq/") (synopsis "Peak-calling and prioritization pipeline for ChIP-Seq data") (description "PePr is a ChIP-Seq peak calling or differential binding analysis tool that is primarily designed for data with biological replicates. It uses a negative binomial distribution to model the read counts among the samples in the same group, and look for consistent differences between ChIP and control group or two ChIP groups run under different conditions.") (license license:gpl3+))) (define-public filevercmp (let ((commit "1a9b779b93d0b244040274794d402106907b71b7")) (package (name "filevercmp") (version (string-append "0-1." (string-take commit 7))) (source (origin (method url-fetch) (uri (string-append "https://github.com/ekg/filevercmp/archive/" commit ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "0yp5jswf5j2pqc6517x277s4s6h1ss99v57kxw9gy0jkfl3yh450")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; There are no tests to run. #:phases (modify-phases %standard-phases (delete 'configure) ; There is no configure phase. (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin"))) (install-file "filevercmp" bin))))))) (home-page "https://github.com/ekg/filevercmp") (synopsis "This program compares version strings") (description "This program compares version strings. It intends to be a replacement for strverscmp.") (license license:gpl3+)))) (define-public multiqc (package (name "multiqc") (version "0.6") (source (origin (method url-fetch) (uri (pypi-uri "multiqc" version)) (sha256 (base32 "0avw11h63ldpxy5pizc3wl1wa01ha7q10wb240nggsjz3jaqvyiy")))) (build-system python-build-system) (propagated-inputs `(("python-jinja2" ,python-jinja2) ("python-simplejson" ,python-simplejson) ("python-pyyaml" ,python-pyyaml) ("python-click" ,python-click) ("python-matplotlib" ,python-matplotlib) ("python-numpy" ,python-numpy))) (native-inputs `(("python-setuptools" ,python-setuptools))) (home-page "http://multiqc.info") (synopsis "Aggregate bioinformatics analysis reports") (description "MultiQC is a tool to aggregate bioinformatics results across many samples into a single report. It contains modules for a large number of common bioinformatics tools.") (license license:gpl3)))